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Cramer, P.; Pesce, C.G.; Baralle, F.E.; Kornblihtt, A.R. "Functional association between promoter structure and transcript alternative splicing" (1997) Proceedings of the National Academy of Sciences of the United States of America. 94(21):11456-11460
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Abstract:

It has been assumed that constitutive and regulated splicing of RNA polymerase II transcripts depends exclusively on signals present in the RNA molecule. Here we show that changes in promoter structure strongly affect splice site selection. We investigated the splicing of the ED I exon, which encodes a facultative type III repeat of fibronectin, whose inclusion is regulated during development and in proliferative processes. We used an alternative splicing assay combined with promoter swapping to demonstrate that the extent of ED I splicing is dependent on the promoter structure from which the transcript originated and that this regulation is independent of the promoter strength. Thus, these results provide the first evidence for coupling between alternative splicing and promoter-specific transcription, which agrees with recent cytological and biochemical evidence of coordination between splicing and transcription.

Registro:

Documento: Artículo
Título:Functional association between promoter structure and transcript alternative splicing
Autor:Cramer, P.; Pesce, C.G.; Baralle, F.E.; Kornblihtt, A.R.
Filiación:Lab. de Fisiol. y Biol. Molecular, Universidad de Buenos Aires, Ciudad Universitaria, (1428) Buenos Aires, Argentina
Intl. Ctr. Genetic Eng. and Biotech., Trieste, Italy
Palabras clave:Fibronectin; Transcription; fibronectin; rna polymerase ii; alternative rna splicing; article; cell proliferation; controlled study; development; gene structure; hepatoma cell; human; human cell; nonhuman; priority journal; promoter region; rna transcription; signal transduction; spliceosome; Alternative Splicing; beta-Galactosidase; Exons; Fibronectins; Globins; Humans; Models, Genetic; Polymerase Chain Reaction; Promoter Regions (Genetics); Recombinant Fusion Proteins; RNA Polymerase II; RNA Precursors; RNA, Messenger; Transcription, Genetic; Transfection; Tumor Cells, Cultured
Año:1997
Volumen:94
Número:21
Página de inicio:11456
Página de fin:11460
DOI: http://dx.doi.org/10.1073/pnas.94.21.11456
Título revista:Proceedings of the National Academy of Sciences of the United States of America
Título revista abreviado:PROC. NATL. ACAD. SCI. U. S. A.
ISSN:00278424
CODEN:PNASA
CAS:beta-Galactosidase, EC 3.2.1.23; Fibronectins; Globins, 9004-22-2; Recombinant Fusion Proteins; RNA Polymerase II, EC 2.7.7.-; RNA Precursors; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v94_n21_p11456_Cramer

Referencias:

  • Xing, Y., Lawrence, J.B., (1993) Trends Cell Biol., 3, pp. 346-353
  • Xing, Y., Johnson, C.V., Dobner, P.R., Lawrence, J.B., (1993) Science, 259, pp. 1326-1330
  • Misteli, T., Cáceres, J.F., Spector, D.L., (1997) Nature (London), 387, pp. 523-526
  • Kornblihtt, A.R., Vibe-Pedersen, K., Baralle, F.E., (1984) EMBO J., 3, pp. 221-226
  • Gutman, A., Kornblihtt, A.R., (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 7179-7182
  • Kornblihtt, A.R., Pesce, C.G., Alonso, C.R., Cramer, P., Srebrow, A., Werbajh, S.E., Muro, A.F., (1996) FASEB J., 10, pp. 248-257
  • Mardon, H.J., Sebastio, G., Baralle, F.E., (1987) Nucleic Acids Res., 15, pp. 7725-7733
  • Caputi, M., Casari, G., Guenzi, S., Tagliabue, R., Sidoli, A., Melo, C.A., Baralle, F.E., (1994) Nucleic Acids Res., 22, pp. 1018-1022
  • Lavigueur, A., La Branche, H., Kornblihtt, A.R., Chabot, B., (1993) Genes Dev., 7, pp. 2405-2417
  • De Simone, V., Ciliberto, G., Hardon, E., Paonessa, G., Palla, F., Lundberg, L., Cortese, R., (1987) EMBO J., 6, pp. 2759-2766
  • Alonso, C.R., Pesce, C.G., Kornblihtt, A.R., (1996) J. Biol. Chem., 271, pp. 22271-22279
  • Chomczynski, P., Sacchi, N., (1987) Anal. Biochem., 162, pp. 156-159
  • Muro, A.F., Bernath, V.A., Kornblihtt, A.R., (1992) J. Biol. Chem., 267, pp. 12767-12774
  • Chabot, B., (1996) Trends Genet., 12, pp. 472-478
  • Roche, S.E., Schiff, M., Rio, D.C., (1995) Genes Dev., 9, pp. 1278-1288
  • Eperon, L.P., Graham, I.R., Griffiths, A.D., Eperon, I.C., (1988) Cell, 54, pp. 393-401
  • Hunninghake, G.W., Monick, M.M., Liu, B., Stinski, M.F., (1989) J. Virol., 63, pp. 3026-3303
  • McKeown, M., (1992) Annu. Rev. Cell Biol., 8, pp. 133-155
  • Sisodia, S.S., Sollner-Webb, B., Cleveland, D.W., (1987) Mol. Cell. Biol., 7, pp. 3601-3612
  • Vincent, M., Lauriault, P., Dubois, M.-F., Lavoie, S., Besaude, O., Chabot, B., (1996) Nucleic Acids Res., 24, pp. 4649-4652
  • Yuryev, A., Patturajan, M., Litingtung, Y., Joshi, R.V., Gentile, C., Gebara, M., Corden, J.L., (1996) Proc. Natl. Acad. Sci. USA, 93, pp. 6975-6980
  • Mc Cracken, S., Fong, N., Yankulov, K., Ballantyne, S., Pan, G., Greenblatt, J., Patterson, S.D., Bentley, D., (1997) Nature (London), 385, pp. 357-361
  • Greenleaf, A., (1993) Trends Biochem. Sci., 18, pp. 117-119
  • Tacke, R., Manley, J.L., (1995) EMBO J., 14, pp. 3540-3551
  • Makela, T.P., Parvin, J.D., Kim, J., Huber, L.J., Sharp, P.A., Weinberg, R., (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 5174-5178
  • Bermingham Jr., J.R., Scott, M.P., (1988) EMBO J., 7, pp. 3211-3222
  • Bergers, G., Reikerstorfer, A., Braselmann, S., Graninger, P., Busslinger, M., (1994) EMBO J., 13, pp. 1176-1188
  • Cáceres, J.F., Stamm, S., Helfman, D.M., Krainer, A.R., (1994) Science, 265, pp. 1706-1709
  • Godowski, P.J., Picard, D., Yamamoto, K.R., (1988) Science, 241, pp. 812-816

Citas:

---------- APA ----------
Cramer, P., Pesce, C.G., Baralle, F.E. & Kornblihtt, A.R. (1997) . Functional association between promoter structure and transcript alternative splicing. Proceedings of the National Academy of Sciences of the United States of America, 94(21), 11456-11460.
http://dx.doi.org/10.1073/pnas.94.21.11456
---------- CHICAGO ----------
Cramer, P., Pesce, C.G., Baralle, F.E., Kornblihtt, A.R. "Functional association between promoter structure and transcript alternative splicing" . Proceedings of the National Academy of Sciences of the United States of America 94, no. 21 (1997) : 11456-11460.
http://dx.doi.org/10.1073/pnas.94.21.11456
---------- MLA ----------
Cramer, P., Pesce, C.G., Baralle, F.E., Kornblihtt, A.R. "Functional association between promoter structure and transcript alternative splicing" . Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 21, 1997, pp. 11456-11460.
http://dx.doi.org/10.1073/pnas.94.21.11456
---------- VANCOUVER ----------
Cramer, P., Pesce, C.G., Baralle, F.E., Kornblihtt, A.R. Functional association between promoter structure and transcript alternative splicing. PROC. NATL. ACAD. SCI. U. S. A. 1997;94(21):11456-11460.
http://dx.doi.org/10.1073/pnas.94.21.11456