Abstract:
It has been assumed that constitutive and regulated splicing of RNA polymerase II transcripts depends exclusively on signals present in the RNA molecule. Here we show that changes in promoter structure strongly affect splice site selection. We investigated the splicing of the ED I exon, which encodes a facultative type III repeat of fibronectin, whose inclusion is regulated during development and in proliferative processes. We used an alternative splicing assay combined with promoter swapping to demonstrate that the extent of ED I splicing is dependent on the promoter structure from which the transcript originated and that this regulation is independent of the promoter strength. Thus, these results provide the first evidence for coupling between alternative splicing and promoter-specific transcription, which agrees with recent cytological and biochemical evidence of coordination between splicing and transcription.
Registro:
Documento: |
Artículo
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Título: | Functional association between promoter structure and transcript alternative splicing |
Autor: | Cramer, P.; Pesce, C.G.; Baralle, F.E.; Kornblihtt, A.R. |
Filiación: | Lab. de Fisiol. y Biol. Molecular, Universidad de Buenos Aires, Ciudad Universitaria, (1428) Buenos Aires, Argentina Intl. Ctr. Genetic Eng. and Biotech., Trieste, Italy
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Palabras clave: | Fibronectin; Transcription; fibronectin; rna polymerase ii; alternative rna splicing; article; cell proliferation; controlled study; development; gene structure; hepatoma cell; human; human cell; nonhuman; priority journal; promoter region; rna transcription; signal transduction; spliceosome; Alternative Splicing; beta-Galactosidase; Exons; Fibronectins; Globins; Humans; Models, Genetic; Polymerase Chain Reaction; Promoter Regions (Genetics); Recombinant Fusion Proteins; RNA Polymerase II; RNA Precursors; RNA, Messenger; Transcription, Genetic; Transfection; Tumor Cells, Cultured |
Año: | 1997
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Volumen: | 94
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Número: | 21
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Página de inicio: | 11456
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Página de fin: | 11460
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DOI: |
http://dx.doi.org/10.1073/pnas.94.21.11456 |
Título revista: | Proceedings of the National Academy of Sciences of the United States of America
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Título revista abreviado: | PROC. NATL. ACAD. SCI. U. S. A.
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ISSN: | 00278424
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CODEN: | PNASA
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CAS: | beta-Galactosidase, EC 3.2.1.23; Fibronectins; Globins, 9004-22-2; Recombinant Fusion Proteins; RNA Polymerase II, EC 2.7.7.-; RNA Precursors; RNA, Messenger
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v94_n21_p11456_Cramer |
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Citas:
---------- APA ----------
Cramer, P., Pesce, C.G., Baralle, F.E. & Kornblihtt, A.R.
(1997)
. Functional association between promoter structure and transcript alternative splicing. Proceedings of the National Academy of Sciences of the United States of America, 94(21), 11456-11460.
http://dx.doi.org/10.1073/pnas.94.21.11456---------- CHICAGO ----------
Cramer, P., Pesce, C.G., Baralle, F.E., Kornblihtt, A.R.
"Functional association between promoter structure and transcript alternative splicing"
. Proceedings of the National Academy of Sciences of the United States of America 94, no. 21
(1997) : 11456-11460.
http://dx.doi.org/10.1073/pnas.94.21.11456---------- MLA ----------
Cramer, P., Pesce, C.G., Baralle, F.E., Kornblihtt, A.R.
"Functional association between promoter structure and transcript alternative splicing"
. Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 21, 1997, pp. 11456-11460.
http://dx.doi.org/10.1073/pnas.94.21.11456---------- VANCOUVER ----------
Cramer, P., Pesce, C.G., Baralle, F.E., Kornblihtt, A.R. Functional association between promoter structure and transcript alternative splicing. PROC. NATL. ACAD. SCI. U. S. A. 1997;94(21):11456-11460.
http://dx.doi.org/10.1073/pnas.94.21.11456