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Abstract:

A peptide from hindguts of the Triatoma hematophagous Chagas insect vector activates adenylyl cyclase activity in Trypanosoma cruzi epimastigote membranes and stimulates the in vitro differentiation of epimastigotes to metacyclic trypomastigotes. Hindguts were obtained from insects fed 2 days earlier with chicken blood. Purification was performed by gel filtration and HPLC on C18 and C4 columns. SDS/PAGE of the purified peptide showed a single band of about 10 kDa. The following sequence was determined for the 20 amino-terminal residues of this peptide: H 2N-Met-Leu-Thr-Ala-Glu-Asp-Lys-Lys-Leu-Ile-Gln-Gln-Ala-Trp-Glu-Lys- Ala-Ala-Ser-His. This sequence is identical to the amino terminus of chicken αD-globin. On a Western blot, the peptide immunoreacted with a polyclonal antibody against chicken globin D. A synthetic peptide corresponding to residues 1-40 of the αD-globin amino terminus also stimulated adenylyl cyclase activity and promoted differentiation. This 125I-labeled synthetic peptide bound specifically to T. cruzi epimastigote cells. Activation of epimastigote adenylyl cyclase by the hemoglobin-derived peptide may play an important role in T. cruzi differentiation and consequently in the transmission of Chagas disease.

Registro:

Documento: Artículo
Título:Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms
Autor:Fraidenraich, D.; Peña, C.; Isola, E.L.; Lammel, E.M.; Coso, O.; Añel, A.D.; Pongor, S.; Baralle, F.; Torres, H.N.; Flawia, M.M.
Filiación:Inst. Invest. Ing. Genet. y Biol. M., Fac. de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina
Inst. de Quim. y Fisicoquimica Biol., Fac. de Farmacia y Bioquímica, 1113 Buenos Aires, Argentina
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, 1113 Buenos Aires, Argentina
Intl. Ctr. Genetic Eng. Biotech.-T., Patriciamo 99, 34012 Trieste, Italy
Inst. Invest. en Ing. Genet. y Biol., Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Gallus gallus; Hexapoda; Insecta; Triatoma; Trypanosoma; Trypanosoma cruzi; Gallus gallus; Hexapoda; Insecta; Triatoma; Trypanosoma; Trypanosoma cruzi; adenylate cyclase; hemoglobin a; amino acid sequence; amino terminal sequence; article; chagas disease; controlled study; enzyme activation; epimastigote; nonhuman; parasite development; parasite transmission; priority journal; triatoma; trypanosoma cruzi; trypomastigote; Adenylate Cyclase; Amino Acid Sequence; Animal; Chickens; Chromatography, High Pressure Liquid; Digestive Physiology; Electrophoresis, Polyacrylamide Gel; Globins; Guanosine Triphosphate; Guanylyl Imidodiphosphate; Hemoglobins; Human; Mice; Molecular Sequence Data; Peptide Fragments; Peptides; Sequence Homology, Amino Acid; Support, Non-U.S. Gov't; Triatoma; Trypanosoma cruzi
Año:1993
Volumen:90
Número:21
Página de inicio:10140
Página de fin:10144
DOI: http://dx.doi.org/10.1073/pnas.90.21.10140
Título revista:Proceedings of the National Academy of Sciences of the United States of America
Título revista abreviado:Proc. Natl. Acad. Sci. U. S. A.
ISSN:00278424
CODEN:PNASA
CAS:Adenylate Cyclase, EC 4.6.1.1; Globins, 9004-22-2; Guanosine Triphosphate, 86-01-1; Guanylyl Imidodiphosphate, 34273-04-6; Hemoglobins; Peptide Fragments; Peptides
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00278424_v90_n21_p10140_Fraidenraich.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v90_n21_p10140_Fraidenraich

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Citas:

---------- APA ----------
Fraidenraich, D., Peña, C., Isola, E.L., Lammel, E.M., Coso, O., Añel, A.D., Pongor, S.,..., Flawia, M.M. (1993) . Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms. Proceedings of the National Academy of Sciences of the United States of America, 90(21), 10140-10144.
http://dx.doi.org/10.1073/pnas.90.21.10140
---------- CHICAGO ----------
Fraidenraich, D., Peña, C., Isola, E.L., Lammel, E.M., Coso, O., Añel, A.D., et al. "Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms" . Proceedings of the National Academy of Sciences of the United States of America 90, no. 21 (1993) : 10140-10144.
http://dx.doi.org/10.1073/pnas.90.21.10140
---------- MLA ----------
Fraidenraich, D., Peña, C., Isola, E.L., Lammel, E.M., Coso, O., Añel, A.D., et al. "Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms" . Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 21, 1993, pp. 10140-10144.
http://dx.doi.org/10.1073/pnas.90.21.10140
---------- VANCOUVER ----------
Fraidenraich, D., Peña, C., Isola, E.L., Lammel, E.M., Coso, O., Añel, A.D., et al. Stimulation of Trypanosoma cruzi adenylyl cyclase by an αD-globin fragment from Triatoma hindgut: Effect on differentiation of epimastigote to trypomastigote forms. Proc. Natl. Acad. Sci. U. S. A. 1993;90(21):10140-10144.
http://dx.doi.org/10.1073/pnas.90.21.10140