Abstract:
Light cues from neighboring vegetation rapidly initiate plant shade-avoidance responses. Despite our detailed knowledge of the early steps of this response, the molecular events under prolonged shade are largely unclear. Here we show that persistent neighbor cues reinforce growth responses in addition to promoting auxin-responsive gene expression in Arabidopsis and soybean. However, while the elevation of auxin levels is well established as an early event, in Arabidopsis, the response to prolonged shade occurs when auxin levels have declined to the pres-timulation values. Remarkably, the sustained low activity of phytochrome B under prolonged shade led to (i) decreased levels of PHYTOCHROME INTERACTING FACTOR 4 (PIF4) in the cotyledons (the organs that supply auxin) along with increased levels in the vascular tissues of the stem, (ii) elevated expression of the PIF4 targets INDOLE-3-ACETIC ACID 19 (IAA19) and IAA29, which in turn reduced the expression of the growth-repressive IAA17 regulator, (iii) reduced abundance of AUXIN RESPONSE FACTOR 6, (iv) reduced expression of MIR393 and increased abundance of its targets, the auxin receptors, and (v) elevated auxin signaling as indicated by molecular markers. Mathematical and genetic analyses support the physiological role of this system-level rearrangement. We propose that prolonged shade rewires the connectivity between light and auxin signaling to sustain shade avoidance without enhanced auxin levels. © 2018 National Academy of Sciences. All Rights Reserved.
Registro:
Documento: |
Artículo
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Título: | Rewiring of auxin signaling under persistent shade |
Autor: | Pucciariello, O.; Legris, M.; Rojas, C.C.; Iglesias, M.J.; Hernando, C.E.; Dezar, C.; Vazquez, M.; Yanovsky, M.J.; Finlayson, S.A.; Prat, S.; Casal, J.J. |
Filiación: | Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA), Facultad de Agronomía, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, CONICET, Buenos Aires, Argentina Instituto de Agrobiotecnología de Rosario, CONICET, Rosario, Argentina Department of Soil and Crop Sciences, Texas A and M University, College Station, TX, United States Faculty of Molecular and Environmental Plant Sciences, Texas A and M University, College Station, TX, United States Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Madrid, Spain Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata and CONICET, Mar del Plata, Argentina
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Palabras clave: | Aux/IAA; Auxin receptor; MIR393; Phytochrome B; PIF4 |
Año: | 2018
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Volumen: | 115
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Número: | 21
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Página de inicio: | 5612
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Página de fin: | 5617
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DOI: |
http://dx.doi.org/10.1073/pnas.1721110115 |
Título revista: | Proceedings of the National Academy of Sciences of the United States of America
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Título revista abreviado: | Proc. Natl. Acad. Sci. U. S. A.
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ISSN: | 00278424
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CODEN: | PNASA
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v115_n21_p5612_Pucciariello |
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Citas:
---------- APA ----------
Pucciariello, O., Legris, M., Rojas, C.C., Iglesias, M.J., Hernando, C.E., Dezar, C., Vazquez, M.,..., Casal, J.J.
(2018)
. Rewiring of auxin signaling under persistent shade. Proceedings of the National Academy of Sciences of the United States of America, 115(21), 5612-5617.
http://dx.doi.org/10.1073/pnas.1721110115---------- CHICAGO ----------
Pucciariello, O., Legris, M., Rojas, C.C., Iglesias, M.J., Hernando, C.E., Dezar, C., et al.
"Rewiring of auxin signaling under persistent shade"
. Proceedings of the National Academy of Sciences of the United States of America 115, no. 21
(2018) : 5612-5617.
http://dx.doi.org/10.1073/pnas.1721110115---------- MLA ----------
Pucciariello, O., Legris, M., Rojas, C.C., Iglesias, M.J., Hernando, C.E., Dezar, C., et al.
"Rewiring of auxin signaling under persistent shade"
. Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 21, 2018, pp. 5612-5617.
http://dx.doi.org/10.1073/pnas.1721110115---------- VANCOUVER ----------
Pucciariello, O., Legris, M., Rojas, C.C., Iglesias, M.J., Hernando, C.E., Dezar, C., et al. Rewiring of auxin signaling under persistent shade. Proc. Natl. Acad. Sci. U. S. A. 2018;115(21):5612-5617.
http://dx.doi.org/10.1073/pnas.1721110115