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Ge, X.N.; Ha, S.G.; Greenberg, Y.G.; Rao, A.; Bastan, I.; Blidner, A.G.; Rao, S.P.; Rabinovich, G.A.; Sriramarao, P. "Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1" (2016) Proceedings of the National Academy of Sciences of the United States of America. 113(33):E4837-E4846
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Abstract:

Galectin-1 (Gal-1), a glycan-binding protein with broad antiinflammatory activities, functions as a proresolving mediator in autoimmune and chronic inflammatory disorders. However, its role in allergic airway inflammation has not yet been elucidated. We evaluated the effects of Gal-1 on eosinophil function and its role in a mouse model of allergic asthma. Allergen exposure resulted in airway recruitment of Gal-1-expressing inflammatory cells, including eosinophils, as well as increased Gal-1 in extracellular spaces in the lungs. In vitro, extracellular Gal-1 exerted divergent effects on eosinophils that were N-glycan- And dose-dependent. At concentrations ≤0.25 μM, Gal-1 increased eosinophil adhesion to vascular cell adhesion molecule-1, caused redistribution of integrin CD49d to the periphery and cell clustering, but inhibited ERK(1/2) activation and eotaxin-1-induced migration. Exposure to concentrations ≥1 μM resulted in ERK(1/2)- dependent apoptosis and disruption of the F- Actin cytoskeleton. At lower concentrations, Gal-1 did not alter expression of adhesion molecules (CD49d, CD18, CD11a, CD11b, L-selectin) or of the chemokine receptor CCR3, but decreased CD49d and CCR3 was observed in eosinophils treated with higher concentrations of this lectin. In vivo, allergen-challenged Gal-1-deficient mice exhibited increased recruitment of eosinophils and CD3+ T lymphocytes in the airways as well as elevated peripheral blood and bone marrow eosinophils relative to corresponding WT mice. Further, these mice had an increased propensity to develop airway hyperresponsiveness and displayed significantly elevated levels of TNF-α in lung tissue. This study suggests that Gal-1 can limit eosinophil recruitment to allergic airways and suppresses airway inflammation by inhibiting cell migration and promoting eosinophil apoptosis.

Registro:

Documento: Artículo
Título:Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1
Autor:Ge, X.N.; Ha, S.G.; Greenberg, Y.G.; Rao, A.; Bastan, I.; Blidner, A.G.; Rao, S.P.; Rabinovich, G.A.; Sriramarao, P.
Filiación:Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN 55108, United States
Laboratorio de Inmunopatología, Instituto de Biología Y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, C1428ADN Buenos Aires, Argentina
Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, C1428EGA Buenos Aires, Argentina
Department of Medicine, University of Minnesota, Minneapolis, MN 55455, United States
Palabras clave:Allergic airway inflammation; Apoptosis; Eosinophils; Galectin-1; Migration; allergen; alpha4 integrin; CD11b antigen; CD18 antigen; cell surface receptor; chemokine receptor CCR3; cytoskeleton protein; eotaxin; eotaxin 2; F actin; galectin 1; glycan; interleukin 13; interleukin 5; L selectin; lymphocyte function associated antigen 1; mitogen activated protein kinase 1; mitogen activated protein kinase 3; transforming growth factor beta; tumor necrosis factor alpha; vascular cell adhesion molecule 1; chemokine; cytokine; galectin 1; allergic asthma; animal cell; animal experiment; animal model; animal tissue; apoptosis; Article; bone marrow cell; CD3+ T lymphocyte; cell adhesion; comparative study; concentration response; controlled study; cytoskeleton; enzyme activation; eosinophil; eosinophilia; extracellular space; immunoregulation; in vitro study; in vivo study; inflammatory cell; leukocyte function; leukocyte migration; lung; migration inhibition; mouse; nonhuman; priority journal; protein expression; protein function; protein localization; protein protein interaction; animal; asthma; C57BL mouse; chemistry; eosinophilia; physiology; Animals; Apoptosis; Asthma; Cell Adhesion; Chemokines; Cytokines; Eosinophilia; Eosinophils; Galectin 1; Lung; Mice; Mice, Inbred C57BL
Año:2016
Volumen:113
Número:33
Página de inicio:E4837
Página de fin:E4846
DOI: http://dx.doi.org/10.1073/pnas.1601958113
Título revista:Proceedings of the National Academy of Sciences of the United States of America
Título revista abreviado:Proc. Natl. Acad. Sci. U. S. A.
ISSN:00278424
CODEN:PNASA
CAS:alpha4 integrin, 355485-74-4; CD18 antigen, 172592-43-7; F actin, 39409-31-9; galectin 1, 258495-34-0; interleukin 13, 148157-34-0; L selectin, 126880-86-2; mitogen activated protein kinase 1, 137632-08-7; mitogen activated protein kinase 3, 137632-07-6; Chemokines; Cytokines; Galectin 1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v113_n33_pE4837_Ge

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Citas:

---------- APA ----------
Ge, X.N., Ha, S.G., Greenberg, Y.G., Rao, A., Bastan, I., Blidner, A.G., Rao, S.P.,..., Sriramarao, P. (2016) . Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1. Proceedings of the National Academy of Sciences of the United States of America, 113(33), E4837-E4846.
http://dx.doi.org/10.1073/pnas.1601958113
---------- CHICAGO ----------
Ge, X.N., Ha, S.G., Greenberg, Y.G., Rao, A., Bastan, I., Blidner, A.G., et al. "Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1" . Proceedings of the National Academy of Sciences of the United States of America 113, no. 33 (2016) : E4837-E4846.
http://dx.doi.org/10.1073/pnas.1601958113
---------- MLA ----------
Ge, X.N., Ha, S.G., Greenberg, Y.G., Rao, A., Bastan, I., Blidner, A.G., et al. "Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1" . Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 33, 2016, pp. E4837-E4846.
http://dx.doi.org/10.1073/pnas.1601958113
---------- VANCOUVER ----------
Ge, X.N., Ha, S.G., Greenberg, Y.G., Rao, A., Bastan, I., Blidner, A.G., et al. Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1. Proc. Natl. Acad. Sci. U. S. A. 2016;113(33):E4837-E4846.
http://dx.doi.org/10.1073/pnas.1601958113