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Abstract:

The α9 and α10 cholinergic nicotinic receptor subunits assemble to form the receptor that mediates efferent inhibition of hair cell function within the auditory sensory organ, a mechanism thought to modulate the dynamic range of hearing. In contrast to all nicotinic receptors, which serve excitatory neurotransmission, the activation of α9α10 produces hyperpolarization of hair cells. An evolutionary analysis has shown that the α10 subunit exhibits signatures of positive selection only along the mammalian lineage, strongly suggesting the acquisition of a unique function. To establish whether mammalian α9α10 receptors have acquired distinct functional properties as a consequence of this evolutionary pressure, we compared the properties of rat and chicken recombinant and native α9α10 receptors. Our main finding in the present work is that, in contrast to the high (pCa 2+/pMonovalents ∼10) Ca 2+ permeability reported for rat α9α10 receptors, recombinant and native chicken α9α10 receptors have a much lower permeability (∼2) to this cation, comparable to that of neuronal α4β2 receptors. Moreover, we show that, in contrast to α10, α7 as well as α4 and β2 nicotinic subunits are under purifying selection in vertebrates, consistent with the conserved Ca 2+ permeability reported across species. These results have important consequences for the activation of signaling cascades that lead to hyperpolarization of hair cells after α9α10 gating at the cholinergic-hair cell synapse. In addition, they suggest that high Ca 2+ permeability of the α9α10 cholinergic nicotinic receptor might have evolved together with other features that have given the mammalian ear an expanded high-frequency sensitivity.

Registro:

Documento: Artículo
Título:Phylogenetic differences in calcium permeability of the auditory hair cell cholinergic nicotinic receptor
Autor:Lipovsek, M.; Im, G.J.; Franchini, L.F.; Pisciottano, F.; Katz, E.; Fuchs, P.A.; Elgoyhen, A.B.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, 1428 Buenos Aires, Argentina
Department of Otolaryngology and Head and Neck Surgery, Center for Hearing and Balance, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Departamento de Farmacología, Facultad de Medicina, Universidad de Buenos Aires, 1121 Buenos Aires, Argentina
Palabras clave:Evolution; Ionotropic receptor; Ligand-gated channel; Outer hair cell; calcium ion; cation; cell receptor; nicotinic receptor alpha4beta2; animal cell; article; artificial chromosome; calcium transport; channel gating; chicken; hair cell; hyperpolarization; mammal; neurotransmission; nonhuman; phylogeny; priority journal; synapse; vertebrate; Acetylcholine; Animals; Calcium; Cell Membrane Permeability; Chickens; Evolution, Molecular; Hair Cells, Auditory; Humans; Likelihood Functions; Oocytes; Phylogeny; Protein Subunits; Rats; Receptors, Nicotinic; Recombinant Proteins; Xenopus laevis; Mammalia; Rattus; Vertebrata
Año:2012
Volumen:109
Número:11
Página de inicio:4308
Página de fin:4313
DOI: http://dx.doi.org/10.1073/pnas.1115488109
Título revista:Proceedings of the National Academy of Sciences of the United States of America
Título revista abreviado:Proc. Natl. Acad. Sci. U. S. A.
ISSN:00278424
CODEN:PNASA
CAS:calcium ion, 14127-61-8; Acetylcholine, 51-84-3; Calcium, 7440-70-2; Protein Subunits; Receptors, Nicotinic; Recombinant Proteins
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00278424_v109_n11_p4308_Lipovsek.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v109_n11_p4308_Lipovsek

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Citas:

---------- APA ----------
Lipovsek, M., Im, G.J., Franchini, L.F., Pisciottano, F., Katz, E., Fuchs, P.A. & Elgoyhen, A.B. (2012) . Phylogenetic differences in calcium permeability of the auditory hair cell cholinergic nicotinic receptor. Proceedings of the National Academy of Sciences of the United States of America, 109(11), 4308-4313.
http://dx.doi.org/10.1073/pnas.1115488109
---------- CHICAGO ----------
Lipovsek, M., Im, G.J., Franchini, L.F., Pisciottano, F., Katz, E., Fuchs, P.A., et al. "Phylogenetic differences in calcium permeability of the auditory hair cell cholinergic nicotinic receptor" . Proceedings of the National Academy of Sciences of the United States of America 109, no. 11 (2012) : 4308-4313.
http://dx.doi.org/10.1073/pnas.1115488109
---------- MLA ----------
Lipovsek, M., Im, G.J., Franchini, L.F., Pisciottano, F., Katz, E., Fuchs, P.A., et al. "Phylogenetic differences in calcium permeability of the auditory hair cell cholinergic nicotinic receptor" . Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 11, 2012, pp. 4308-4313.
http://dx.doi.org/10.1073/pnas.1115488109
---------- VANCOUVER ----------
Lipovsek, M., Im, G.J., Franchini, L.F., Pisciottano, F., Katz, E., Fuchs, P.A., et al. Phylogenetic differences in calcium permeability of the auditory hair cell cholinergic nicotinic receptor. Proc. Natl. Acad. Sci. U. S. A. 2012;109(11):4308-4313.
http://dx.doi.org/10.1073/pnas.1115488109