Abstract:
The mechanisms underlying the biosynthesis of cellulose in plants are complex and still poorly understood. A central question concerns the mechanism of microfibril structure and how this is linked to the catalytic polymerization action of cellulose synthase (CESA). Furthermore, it remains unclear whether modification of cellulose microfibril structure can be achieved genetically, which could be transformative in a bio-based economy. To explore these processes in planta, we developed a chemical genetic toolbox of pharmacological inhibitors and corresponding resistance-conferring point mutations in the C-terminal transmembrane domain region of CESA1 A903V and CESA3 T942I in Arabidopsis thaliana. Using 13C solidstate nuclear magnetic resonance spectroscopy and X-ray diffraction, we show that the cellulose microfibrils displayed reduced width and an additional cellulose C4 peak indicative of a degree of crystallinity that is intermediate between the surface and interior glucans of wild type, suggesting a difference in glucan chain association during microfibril formation. Consistent with measurements of lower microfibril crystallinity, cellulose extracts from mutated CESA1 A903V and CESA3 T942I displayed greater saccharification efficiency than wild type. Using live-cell imaging to track fluorescently labeled CESA, we found that these mutants show increased CESA velocities in the plasma membrane, an indication of increased polymerization rate. Collectively, these data suggest that CESA1 A903Vand CESA3 T942I have modified microfibril structure in terms of crystallinity and suggest that in plants, as in bacteria, crystallization biophysically limits polymerization.
Registro:
| Documento: |
Artículo
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| Título: | Cellulose microfibril crystallinity is reduced by mutating C-terminal transmembrane region residues CESA1 A903V and CESA3 T942I of cellulose synthase |
| Autor: | Harris, D.M.; Corbin, K.; Wang, T.; Gutierrez, R.; Bertolo, A.L.; Petti, C.; Smilgies, D.-M.; Estevez, J.M.; Bonetta, D.; Urbanowicz, B.R.; Ehrhardt, D.W.; Somerville, C.R.; Rose, J.K.C.; Hong, M.; DeBolt, S. |
| Filiación: | Department of Horticulture, University of Kentucky, Lexington, KY 40546, United States
Department of Chemistry and Ames Laboratory, Iowa State University, Ames, IA 50011, United States
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, United States
Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Cornell High Energy Synchrotron Source (CHESS) Wilson Laboratory, Cornell University, Ithaca, NY 14853, United States
Instituto de Fisiología, Biología Molecular y Neurociencias, Universidad de Buenos Aires, Buenos Aires C1428EGA, Argentina
Faculty of Science, University of Ontario Institute of Technology, ON L1H 7K4, Canada
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States
Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, United States
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| Palabras clave: | Cell wall; Polysaccharide; Quinoxyphen; cellulose; hemicellulose; allelism; Arabidopsis; article; carbon nuclear magnetic resonance; cell membrane; cell wall; crystallization; cytoskeleton; fiber; fibrin polymerization; germination; IC 50; nonhuman; plant growth; plant stem; point mutation; priority journal; saccharification; X ray diffraction; Alleles; Amino Acid Sequence; Amino Acid Substitution; Arabidopsis; Arabidopsis Proteins; Cell Membrane; Cellulose; Crystallization; Drug Resistance; Genes, Dominant; Glucosyltransferases; Magnetic Resonance Spectroscopy; Microfibrils; Molecular Sequence Data; Mutant Proteins; Mutation; Protein Transport; Quinolines; Structure-Activity Relationship; Arabidopsis thaliana |
| Año: | 2012
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| Volumen: | 109
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| Número: | 11
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| Página de inicio: | 4098
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| Página de fin: | 4103
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| DOI: |
http://dx.doi.org/10.1073/pnas.1200352109 |
| Título revista: | Proceedings of the National Academy of Sciences of the United States of America
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| Título revista abreviado: | Proc. Natl. Acad. Sci. U. S. A.
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| ISSN: | 00278424
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| CODEN: | PNASA
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| CAS: | cellulose, 61991-22-8, 68073-05-2, 9004-34-6; hemicellulose, 63100-39-0, 63100-40-3, 9034-32-6; Arabidopsis Proteins; CESA1 protein, Arabidopsis, 2.4.1.-; Cellulose, 9004-34-6; CesA3 protein, Arabidopsis, 2.4.1.-; Glucosyltransferases, 2.4.1.-; Mutant Proteins; Quinolines; quinoxyfen
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| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v109_n11_p4098_Harris |
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Citas:
---------- APA ----------
Harris, D.M., Corbin, K., Wang, T., Gutierrez, R., Bertolo, A.L., Petti, C., Smilgies, D.-M.,..., DeBolt, S.
(2012)
. Cellulose microfibril crystallinity is reduced by mutating C-terminal transmembrane region residues CESA1 A903V and CESA3 T942I of cellulose synthase. Proceedings of the National Academy of Sciences of the United States of America, 109(11), 4098-4103.
http://dx.doi.org/10.1073/pnas.1200352109---------- CHICAGO ----------
Harris, D.M., Corbin, K., Wang, T., Gutierrez, R., Bertolo, A.L., Petti, C., et al.
"Cellulose microfibril crystallinity is reduced by mutating C-terminal transmembrane region residues CESA1 A903V and CESA3 T942I of cellulose synthase"
. Proceedings of the National Academy of Sciences of the United States of America 109, no. 11
(2012) : 4098-4103.
http://dx.doi.org/10.1073/pnas.1200352109---------- MLA ----------
Harris, D.M., Corbin, K., Wang, T., Gutierrez, R., Bertolo, A.L., Petti, C., et al.
"Cellulose microfibril crystallinity is reduced by mutating C-terminal transmembrane region residues CESA1 A903V and CESA3 T942I of cellulose synthase"
. Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 11, 2012, pp. 4098-4103.
http://dx.doi.org/10.1073/pnas.1200352109---------- VANCOUVER ----------
Harris, D.M., Corbin, K., Wang, T., Gutierrez, R., Bertolo, A.L., Petti, C., et al. Cellulose microfibril crystallinity is reduced by mutating C-terminal transmembrane region residues CESA1 A903V and CESA3 T942I of cellulose synthase. Proc. Natl. Acad. Sci. U. S. A. 2012;109(11):4098-4103.
http://dx.doi.org/10.1073/pnas.1200352109
