Abstract:
Protein modification by conjugation of small ubiquitin-related modifier (SUMO) is involved in diverse biological functions, such as transcription regulation, subcellular partitioning, stress response, DNA damage repair, and chromatin remodeling. Here, we show that the serine/arginine-rich protein SF2/ASF, a factor involved in splicing regulation and other RNA metabolism-related processes, is a regulator of the sumoylation pathway. The overexpression of this protein stimulates, but its knockdown inhibits SUMO conjugation. SF2/ASF interacts with Ubc9 and enhances sumoylation of specific substrates, sharing characteristics with already described SUMO E3 ligases. In addition, SF2/ASF interacts with the SUMO E3 ligase PIAS1 (protein inhibitor of activated STAT-1), regulating PIAS1-induced overall protein sumoylation. The RNA recognition motif 2 of SF2/ASF is necessary and sufficient for sumoylation enhancement. Moreover, SF2/ASF has a role in heat shock-induced sumoylation and promotes SUMO conjugation to RNA processing factors. These results add a component to the sumoylation pathway and a previously unexplored role for the multifunctional SR protein SF2/ASF.
Registro:
Documento: |
Artículo
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Título: | The serine/arginine-rich protein SF2/ASF regulates protein sumoylation |
Autor: | Pelisch, F.; Gerez, J.; Druker, J.; Schor, I.E.; Muñoz, M.J.; Risso, G.; Petrillo, E.; Westman, B.J.; Lamond, A.I.; Arzt, E.; Srebrow, A. |
Filiación: | Laboratorio de Fisiología Y Biología Molecular, Instituto de Fisiología, Biologia Molecular Y Neurociencias, Consejo Nacional de Investigaciones Cientificas Y Tecnicas, Argentina Departamento de Fisiología, Biología Molecular Y Celular, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Pabellón II, C1428EHA Buenos Aires, Argentina College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
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Palabras clave: | E3 ligase; Posttranslational modification; RNA processing; Splicing factor; protein asf; protein inhibitor of activated STAT; protein inhibitor of activated STAT1; protein sf2; protein Ubc9; regulator protein; RNA; SUMO E3 ligase; SUMO protein; ubiquitin protein ligase E3; unclassified drug; nuclear protein; protein binding; protein p53; RNA binding protein; serine-arginine-rich splicing proteins; small interfering RNA; SUMO 1 protein; ubiquitin conjugating enzyme; ubiquitin-conjugating enzyme UBC9; article; conjugation; controlled study; enzyme activity; heat shock; human; human cell; molecular recognition; priority journal; protein expression; protein function; protein processing; protein protein interaction; regulatory mechanism; RNA metabolism; RNA splicing; signal transduction; sumoylation; cell line; enzyme specificity; genetics; heat shock response; metabolism; Cell Line; Heat-Shock Response; Humans; Nuclear Proteins; Protein Binding; RNA, Small Interfering; RNA-Binding Proteins; Substrate Specificity; SUMO-1 Protein; Tumor Suppressor Protein p53; Ubiquitin-Conjugating Enzymes |
Año: | 2010
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Volumen: | 107
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Número: | 37
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Página de inicio: | 16119
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Página de fin: | 16124
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DOI: |
http://dx.doi.org/10.1073/pnas.1004653107 |
Título revista: | Proceedings of the National Academy of Sciences of the United States of America
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Título revista abreviado: | Proc. Natl. Acad. Sci. U. S. A.
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ISSN: | 00278424
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CODEN: | PNASA
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CAS: | RNA, 63231-63-0; SUMO 1 protein, 182213-10-1; ubiquitin conjugating enzyme, 147154-16-3; Nuclear Proteins; RNA, Small Interfering; RNA-Binding Proteins; serine-arginine-rich splicing proteins; SUMO-1 Protein; Tumor Suppressor Protein p53; ubiquitin-conjugating enzyme UBC9; Ubiquitin-Conjugating Enzymes
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PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00278424_v107_n37_p16119_Pelisch.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v107_n37_p16119_Pelisch |
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Citas:
---------- APA ----------
Pelisch, F., Gerez, J., Druker, J., Schor, I.E., Muñoz, M.J., Risso, G., Petrillo, E.,..., Srebrow, A.
(2010)
. The serine/arginine-rich protein SF2/ASF regulates protein sumoylation. Proceedings of the National Academy of Sciences of the United States of America, 107(37), 16119-16124.
http://dx.doi.org/10.1073/pnas.1004653107---------- CHICAGO ----------
Pelisch, F., Gerez, J., Druker, J., Schor, I.E., Muñoz, M.J., Risso, G., et al.
"The serine/arginine-rich protein SF2/ASF regulates protein sumoylation"
. Proceedings of the National Academy of Sciences of the United States of America 107, no. 37
(2010) : 16119-16124.
http://dx.doi.org/10.1073/pnas.1004653107---------- MLA ----------
Pelisch, F., Gerez, J., Druker, J., Schor, I.E., Muñoz, M.J., Risso, G., et al.
"The serine/arginine-rich protein SF2/ASF regulates protein sumoylation"
. Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 37, 2010, pp. 16119-16124.
http://dx.doi.org/10.1073/pnas.1004653107---------- VANCOUVER ----------
Pelisch, F., Gerez, J., Druker, J., Schor, I.E., Muñoz, M.J., Risso, G., et al. The serine/arginine-rich protein SF2/ASF regulates protein sumoylation. Proc. Natl. Acad. Sci. U. S. A. 2010;107(37):16119-16124.
http://dx.doi.org/10.1073/pnas.1004653107