Pelisch, F.; Gerez, J.; Druker, J.; Schor, I.E.; Muñoz, M.J.; Risso, G.; Petrillo, E.; Westman, B.J.; Lamond, A.I.; Arzt, E.; Srebrow, A. "The serine/arginine-rich protein SF2/ASF regulates protein sumoylation" (2010) Proceedings of the National Academy of Sciences of the United States of America. 107(37):16119-16124
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Protein modification by conjugation of small ubiquitin-related modifier (SUMO) is involved in diverse biological functions, such as transcription regulation, subcellular partitioning, stress response, DNA damage repair, and chromatin remodeling. Here, we show that the serine/arginine-rich protein SF2/ASF, a factor involved in splicing regulation and other RNA metabolism-related processes, is a regulator of the sumoylation pathway. The overexpression of this protein stimulates, but its knockdown inhibits SUMO conjugation. SF2/ASF interacts with Ubc9 and enhances sumoylation of specific substrates, sharing characteristics with already described SUMO E3 ligases. In addition, SF2/ASF interacts with the SUMO E3 ligase PIAS1 (protein inhibitor of activated STAT-1), regulating PIAS1-induced overall protein sumoylation. The RNA recognition motif 2 of SF2/ASF is necessary and sufficient for sumoylation enhancement. Moreover, SF2/ASF has a role in heat shock-induced sumoylation and promotes SUMO conjugation to RNA processing factors. These results add a component to the sumoylation pathway and a previously unexplored role for the multifunctional SR protein SF2/ASF.


Documento: Artículo
Título:The serine/arginine-rich protein SF2/ASF regulates protein sumoylation
Autor:Pelisch, F.; Gerez, J.; Druker, J.; Schor, I.E.; Muñoz, M.J.; Risso, G.; Petrillo, E.; Westman, B.J.; Lamond, A.I.; Arzt, E.; Srebrow, A.
Filiación:Laboratorio de Fisiología Y Biología Molecular, Instituto de Fisiología, Biologia Molecular Y Neurociencias, Consejo Nacional de Investigaciones Cientificas Y Tecnicas, Argentina
Departamento de Fisiología, Biología Molecular Y Celular, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Pabellón II, C1428EHA Buenos Aires, Argentina
College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
Palabras clave:E3 ligase; Posttranslational modification; RNA processing; Splicing factor; protein asf; protein inhibitor of activated STAT; protein inhibitor of activated STAT1; protein sf2; protein Ubc9; regulator protein; RNA; SUMO E3 ligase; SUMO protein; ubiquitin protein ligase E3; unclassified drug; nuclear protein; protein binding; protein p53; RNA binding protein; serine-arginine-rich splicing proteins; small interfering RNA; SUMO 1 protein; ubiquitin conjugating enzyme; ubiquitin-conjugating enzyme UBC9; article; conjugation; controlled study; enzyme activity; heat shock; human; human cell; molecular recognition; priority journal; protein expression; protein function; protein processing; protein protein interaction; regulatory mechanism; RNA metabolism; RNA splicing; signal transduction; sumoylation; cell line; enzyme specificity; genetics; heat shock response; metabolism; Cell Line; Heat-Shock Response; Humans; Nuclear Proteins; Protein Binding; RNA, Small Interfering; RNA-Binding Proteins; Substrate Specificity; SUMO-1 Protein; Tumor Suppressor Protein p53; Ubiquitin-Conjugating Enzymes
Página de inicio:16119
Página de fin:16124
Título revista:Proceedings of the National Academy of Sciences of the United States of America
Título revista abreviado:Proc. Natl. Acad. Sci. U. S. A.
CAS:RNA, 63231-63-0; SUMO 1 protein, 182213-10-1; ubiquitin conjugating enzyme, 147154-16-3; Nuclear Proteins; RNA, Small Interfering; RNA-Binding Proteins; serine-arginine-rich splicing proteins; SUMO-1 Protein; Tumor Suppressor Protein p53; ubiquitin-conjugating enzyme UBC9; Ubiquitin-Conjugating Enzymes


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---------- APA ----------
Pelisch, F., Gerez, J., Druker, J., Schor, I.E., Muñoz, M.J., Risso, G., Petrillo, E.,..., Srebrow, A. (2010) . The serine/arginine-rich protein SF2/ASF regulates protein sumoylation. Proceedings of the National Academy of Sciences of the United States of America, 107(37), 16119-16124.
---------- CHICAGO ----------
Pelisch, F., Gerez, J., Druker, J., Schor, I.E., Muñoz, M.J., Risso, G., et al. "The serine/arginine-rich protein SF2/ASF regulates protein sumoylation" . Proceedings of the National Academy of Sciences of the United States of America 107, no. 37 (2010) : 16119-16124.
---------- MLA ----------
Pelisch, F., Gerez, J., Druker, J., Schor, I.E., Muñoz, M.J., Risso, G., et al. "The serine/arginine-rich protein SF2/ASF regulates protein sumoylation" . Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 37, 2010, pp. 16119-16124.
---------- VANCOUVER ----------
Pelisch, F., Gerez, J., Druker, J., Schor, I.E., Muñoz, M.J., Risso, G., et al. The serine/arginine-rich protein SF2/ASF regulates protein sumoylation. Proc. Natl. Acad. Sci. U. S. A. 2010;107(37):16119-16124.