Artículo
Abstract:
In many cell-signaling pathways, information is transmitted by the diffusion of messenger molecules. Diffusion coefficients characterize the messenger's spatial range and the characteristic times of signal propagation. Inside cells, particles usually diffuse in the presence of immobile binding sites (or traps). It is well known that binding to traps results in an effective diffusion coefficient that is smaller than the free coefficient in media free of traps. To measure effective diffusion coefficients in cells, "tagged" particles are often used. Radioactive calcium was used in a giant squid axon and in cytosolic extracts of Xenopus laevis oocytes. Fluorescence recovery after photobleaching yields diffusion coefficients from observations of the distribution of fluorescently labeled proteins. In the absence of traps, free diffusion coefficients give both the rate at which single-particle mean square displacements increase and the rate at which information in the form of inhomogeneities in particle concentration spread out with time. We show here that, in the presence of traps, information diffuses faster than single particles. Thus, messages diffuse faster than messengers. Tagged-particle experiments give the single-particle diffusion coefficients and, thus, can underestimate the rate of diffusive signal propagation. © 2006 by The National Academy of Sciences of the USA.
Registro:
| Documento: | Artículo |
| Título: | Messages diffuse faster than messengers |
| Autor: | Pando, B.; Dawson, S.P.; Mak, D.-O.D.; Pearson, J.E. |
| Filiación: | Department of Physics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States Departamento de Física, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina Department of Physiology, University of Pennsylvania, B400 Richards Buildings, 3700 Hamilton Walk, Philadelphia, PA 19104-6085, United States Theoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM 87545, United States |
| Palabras clave: | Binding; Effective diffusion; Fluorescence recovery after photobleaching; Tagged particles; Traps; calcium; carrier protein; fluorescent dye; article; binding site; calculation; diffusion coefficient; fluorescence recovery after photobleaching; mathematical analysis; nonhuman; oocyte; priority journal; signal transduction; time; Xenopus laevis; Animals; Fluorescence; Signal Transduction; Xenopus laevis; Cephalopoda; Xenopus laevis |
| Año: | 2006 |
| Volumen: | 103 |
| Número: | 14 |
| Página de inicio: | 5338 |
| Página de fin: | 5342 |
| DOI: | http://dx.doi.org/10.1073/pnas.0509576103 |
| Título revista: | Proceedings of the National Academy of Sciences of the United States of America |
| Título revista abreviado: | Proc. Natl. Acad. Sci. U. S. A. |
| ISSN: | 00278424 |
| CODEN: | PNASA |
| CAS: | calcium, 7440-70-2; carrier protein, 80700-39-6 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v103_n14_p5338_Pando |
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Citas:
---------- APA ----------
Pando, B., Dawson, S.P., Mak, D.-O.D. & Pearson, J.E. (2006) . Messages diffuse faster than messengers. Proceedings of the National Academy of Sciences of the United States of America, 103(14), 5338-5342.http://dx.doi.org/10.1073/pnas.0509576103
---------- CHICAGO ----------
Pando, B., Dawson, S.P., Mak, D.-O.D., Pearson, J.E. "Messages diffuse faster than messengers" . Proceedings of the National Academy of Sciences of the United States of America 103, no. 14 (2006) : 5338-5342.http://dx.doi.org/10.1073/pnas.0509576103
---------- MLA ----------
Pando, B., Dawson, S.P., Mak, D.-O.D., Pearson, J.E. "Messages diffuse faster than messengers" . Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 14, 2006, pp. 5338-5342.http://dx.doi.org/10.1073/pnas.0509576103
---------- VANCOUVER ----------
Pando, B., Dawson, S.P., Mak, D.-O.D., Pearson, J.E. Messages diffuse faster than messengers. Proc. Natl. Acad. Sci. U. S. A. 2006;103(14):5338-5342.http://dx.doi.org/10.1073/pnas.0509576103
