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Abstract:

Transmission at the mouse neuromuscular junction normally relies on P/Q-type channels, but became jointly dependent on both N-and R-type Ca2+ channels when the P/Q-type channel α1A subunit was deleted. R-type channels lay close to Ca2+ sensors for exocytosis and IK(Ca) channel activation, like the P/Q-type channels they replaced. In contrast, N-type channels were less well localized, but abundant enough to influence secretion strongly, particularly when action potentials were prolonged. Our data suggested that active zone structures may select among multiple Ca2+ channels in the hierarchy P/Q>R>N. The α1A-/- neuromuscular junction displayed several other differences from wild-type: lowered quantal content but greater ability to withstand reductions in the Ca2+/Mg2+ ratio, and little or no paired-pulse facilitation, the latter findings possibly reflecting compensatory mechanisms at individual release sites. Changes in presynaptic function were also associated with a significant reduction in the size of postsynaptic acetylcholine receptor clusters.

Registro:

Documento: Artículo
Título:Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels
Autor:Urbano, F.J.; Piedras-Rentería, E.S.; Jun, K.; Shin, H.-S.; Uchitel, O.D.; Tsien, R.W.
Filiación:Department of Cellular Physiology, Stanford Univ. School of Medicine, Stanford, CA 94305, United States
Lab. de Fisiol. Biol. Molec. y Cel., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, (C1428EHA)-Buenos Aires, Argentina
Cell/Gene Therapy Research Institute, College of Medicine, Pochon CHA University, Seoul 198-1, South Korea
Natl. CRI Ctr. for Calcium/Learning, Korea Inst. of Sci. and Technology, Seoul 130-650, South Korea
Department of Physiology, New York Univ. School of Medicine, 550 First Avenue, New York, NY 10016, United States
Department of Molecular Physiology, Loyola University Chicago, Maywood, IL 60153, United States
Palabras clave:α1A knockout; Ca2+-activated potassium channel; Neuromuscular junction; Paired-pulse facilitation; SNX-482; calcium channel; calcium ion; cholinergic receptor; magnesium ion; action potential; animal cell; article; channel gating; exocytosis; mouse; nerve ending; neuromuscular synapse; neurotransmission; neurotransmitter release; nonhuman; priority journal; 4-Aminopyridine; Animals; Calcium Channels, N-Type; Calcium Channels, P-Type; Calcium Channels, Q-Type; Calcium Channels, R-Type; Mice; Mice, Knockout; Models, Neurological; Motor Endplate; Neuronal Plasticity; Neurotransmitter Agents; Synaptic Transmission; Animalia
Año:2003
Volumen:100
Número:6
Página de inicio:3491
Página de fin:3496
DOI: http://dx.doi.org/10.1073/pnas.0437991100
Título revista:Proceedings of the National Academy of Sciences of the United States of America
Título revista abreviado:Proc. Natl. Acad. Sci. U. S. A.
ISSN:00278424
CODEN:PNASA
CAS:calcium ion, 14127-61-8; magnesium ion, 22537-22-0; 4-Aminopyridine, 504-24-5; Calcium Channels, N-Type; Calcium Channels, P-Type; Calcium Channels, Q-Type; Calcium Channels, R-Type; Neurotransmitter Agents
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00278424_v100_n6_p3491_Urbano.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00278424_v100_n6_p3491_Urbano

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Citas:

---------- APA ----------
Urbano, F.J., Piedras-Rentería, E.S., Jun, K., Shin, H.-S., Uchitel, O.D. & Tsien, R.W. (2003) . Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels. Proceedings of the National Academy of Sciences of the United States of America, 100(6), 3491-3496.
http://dx.doi.org/10.1073/pnas.0437991100
---------- CHICAGO ----------
Urbano, F.J., Piedras-Rentería, E.S., Jun, K., Shin, H.-S., Uchitel, O.D., Tsien, R.W. "Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels" . Proceedings of the National Academy of Sciences of the United States of America 100, no. 6 (2003) : 3491-3496.
http://dx.doi.org/10.1073/pnas.0437991100
---------- MLA ----------
Urbano, F.J., Piedras-Rentería, E.S., Jun, K., Shin, H.-S., Uchitel, O.D., Tsien, R.W. "Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels" . Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 6, 2003, pp. 3491-3496.
http://dx.doi.org/10.1073/pnas.0437991100
---------- VANCOUVER ----------
Urbano, F.J., Piedras-Rentería, E.S., Jun, K., Shin, H.-S., Uchitel, O.D., Tsien, R.W. Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels. Proc. Natl. Acad. Sci. U. S. A. 2003;100(6):3491-3496.
http://dx.doi.org/10.1073/pnas.0437991100