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Abstract:

Saccobolus saccoboloides, a coprophilous fungus, was grown in synthetic liquid media. Extracellular β-glucosidase, β-1,4 endoglucanase and β-1,4 exoglucanase induction and repression by carbohydrates were investigated. When endoglucanase and exoglucanase could not be measured, an alternative carboxymethylcellulose-clearing method was used. Crystalline cellulose was the best inducer, followed by lactose. The mixture of both inducers had an unexpected negative effect. Cellobiose or glucose repressed cellulase production in cellulose containing media. This repression was selective for some components of the cellulase complex, and was not observed using repressor concentrations below 3% w/v. Moreover, the degree of repression varied with the age of the cultures. Cellulase production by this fungus is inducible and subject to a complex repression by easily metabolized sugars.

Registro:

Documento: Artículo
Título:Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates
Autor:Magnelli, P.; Forchiassin, F.
Filiación:Lab. de Micología Exp., Depto. de Ciencias Biológicas, Ciudad Universitaria P2, (1428) Buenos Aires, Argentina
Palabras clave:Ascobolaceae; Endoglucanase; Exoglucanase; β-glucosidase; beta 1,4 endoglucanase; beta 1,4 exoglucanase; beta glucosidase; carboxymethylcellulose; cellobiose; cellulase; cellulose; culture medium; enzyme induction; enzyme synthesis; glucose; lactose; Saccobolus saccoboloides
Año:1999
Volumen:91
Número:2
Página de inicio:359
Página de fin:364
DOI: http://dx.doi.org/10.2307/3761382
Título revista:Mycologia
Título revista abreviado:Mycologia
ISSN:00275514
CODEN:MYCOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v91_n2_p359_Magnelli

Referencias:

  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254
  • Cotoras, M., Agosin, E., Regulatory aspects of endoglucanase production by the brown-rot fungus Gloeophyllum trabeum (1992) Exp Mycol, 16, pp. 253-260
  • Evans, C.S., Dutton, M.V., Guillen, F., Veness, R.G., Enzymes and small molecular mass agents involved with lignocellulose degradation (1994) FEMS Microbiol Rev, 13, pp. 235-240
  • Gaikwad, J.S., Maheshwari, R., Localization and release of β-glucosidase in the thermophilic and cellulolytic fungus, Sporotrichum thermophile (1994) Exp Mycol, 18, pp. 300-310
  • Hulme, M.A., Stranks, D.W., Regulation of cellulase production by Myrothecium verrucaria grown on non cellulosic substrates (1971) J Gen Microbiol, 69, pp. 145-155
  • Kubicek, C.P., Messner, R., Gruber, F., Mach, R.L., Kubicek Pranz, E.M., The Trichoderma cellulase regulatory puzzle: From the interior life of a secretory fungus (1993) Enzyme Microbial Technol, 15, pp. 90-99
  • Levin, L.N., Sívori, A.S., Pardo, A.G., Actividad celulolítica de hongos coprófilos (1996) Rev Argent Microbiol, 28, pp. 132-138
  • Magnelli, P.E., Ramos, A.M., Forchiassin, F., Factors influencing cellulase production by Saccobolus saccoboloides (1996) Mycologia, 88, pp. 249-255
  • Martínez, A., Mercuri, O.A., Método simple para determinar actividad celulolítica en hongos (1997) Rev Argent Microbiol, 29, pp. 210-214
  • Manning, K., Wood, D.A., Production and regulation of extracellular endocellulase by Agaricus bisporus (1983) J Gen Microbiol, 129, pp. 1839-1847
  • Messner, R., Kubicek, C.P., Carbon source control of CBH I and II formation by Trichoderma reesei (1991) Appl Environ Microbiol, 57, pp. 630-635
  • Nelson, N.J., A photometric adaptation of the Somogyi method for the determination of glucose (1944) J Biochem, 153, pp. 375-380
  • Oguntimein, G.B., Moo-Young, M., Production and properties of β-glucosidase by Neurospora sitophila (1991) World J Microbiol Biotechnol, 7, pp. 4-11
  • Pardo, A.G., Sívori, A.S., Ranalli, M.E., Comparative study of cellulolytic enzyme zymograms of species of Thecoteus and Iodophanus (Pezizales-Ascomycetes) (1997) Mycotaxon, 63, pp. 269-286
  • Ramos, A.M., Forchiassin, F., Producción de endoglucanasa en cuatro especies del género Saccobolus (1996) Rev Argent Microbiol, 28, pp. 55-62
  • Royer, J.C., Nakas, J.P., Interrelationship of xylanase induction and cellulase induction of Trichoderma longibranchiatum (1990) Appl Environ Microbiol, 56, pp. 2535-2539
  • Sandhu, D.K., Kalra, M.K., Effect of cultural conditions on production of cellulases in Trichoderma longibranchiatum (1985) Trans Br Mycol Soc, 84, pp. 251-258
  • Sanyal, A., Kundu, R.K., Sinha, S.N., Dube, D.K., Extracellular cellulolytic enzyme system of Aspergillus japonicus. I Effect of different carbon sources (1988) Enzyme Microbial Technol, 10, pp. 85-90
  • Sívori, A.S., Mercuri, O.A., Forchiassin, F., Cinética de producción de xilanasas y celulasas por Ascobolus gamundii (Fungi, Ascomycotina) (1996) Rev Argent Microbiol, 28, pp. 9-15
  • Somogyi, M.J., Notes on sugar determination (1952) Biol Chem, 159, pp. 19-23
  • Sternberg, D., Mandels, G.R., β-Glucosidase induction and repression in the cellulolytic fungus Trichoderma reesei (1982) Exp Mycol, 6, pp. 115-124
  • Stewart, J.C., Parry, J.B., Factors influencing the production of cellulases by Aspergillus fumigatus (Fresenius) (1981) J Gen Microbiol, 125, pp. 33-39
  • Taj-Aldeen, S.J., Al-Habbeb, E.K., Abdullah, S.K., Cellulolytic activity of coprophilous fungi (1990) Crypt Bot, 2, pp. 25-29
  • Wicklow, D.T., Detroy, R.W., Adams, S., Differential modification of the lignin and cellulose components in wheat straw by fungal colonists of ruminant dung: Ecological implications (1979) Mycologia, 72, pp. 1065-1076
  • Ximenes, E.A., Felix, C.R., Ulhoa, C.J., Production of cellulases by Aspergillus fumigatus and characterization of one β-glucosidase (1996) Curr Microbiol, 32, pp. 119-123

Citas:

---------- APA ----------
Magnelli, P. & Forchiassin, F. (1999) . Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates. Mycologia, 91(2), 359-364.
http://dx.doi.org/10.2307/3761382
---------- CHICAGO ----------
Magnelli, P., Forchiassin, F. "Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates" . Mycologia 91, no. 2 (1999) : 359-364.
http://dx.doi.org/10.2307/3761382
---------- MLA ----------
Magnelli, P., Forchiassin, F. "Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates" . Mycologia, vol. 91, no. 2, 1999, pp. 359-364.
http://dx.doi.org/10.2307/3761382
---------- VANCOUVER ----------
Magnelli, P., Forchiassin, F. Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates. Mycologia. 1999;91(2):359-364.
http://dx.doi.org/10.2307/3761382