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Abstract:

In this work we performed morphological and molecular phylogenetic analyses (based on sequences of calmodulin M [calM], translationelongation factor 1-α [tefA] and b-tubulin [tubB] genes) to characterize the diversity of Epichloë endophytes in Bromus setifolius and Phleum alpinum. The phylogenies obtained from the three genes were congruent and allowed differentiation of three lineages of endophytes that also presented morphological differences. One lineage corresponds to the previously described species Epichloë tembladerae, which is present in a wide range of native grasses from Argentina including B. setifolius and P. alpinum. Another genotype isolated only from B. setifolius is a non-hybrid endophyte, a rare condition for the South American Epichloë endophytes. Isolates of this genotype, described herein as a new variety, Epichloë typhina var. aonikenkana, presented waxy colonies at maturity and a low production of conidia. The third lineage, exclusively found in isolates from P. alpinum, is a hybrid between E. typhina and a common ancestor of E. amarillans and E. baconii. Isolates of this lineage produce abundant conidia that are variable in shape and size. Based on its unique phylogenetic position and morphology, we propose the new species, Epichloë cabralii for this lineage. The new combinations Epichloë tembladerae and E. pampeana also are proposed for the previously described Neotyphodium tembladerae and Neotyphodium pampeanum species. © 2014 by The Mycological Society of America.

Registro:

Documento: Artículo
Título:Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia
Autor:McCargo, P.D.; Iannone, L.J.; Vignale, M.V.; Schardl, C.L.; Rossi, M.S.
Filiación:Laboratorio de Micología, Department de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and Proplame-Prhideb-Conicet, Argentina
Laboratorio de Microbiología Industrial, Department de Ingeniería Química, Facultad de Ingeniería, Argentina
Department of Plant Pathology, University of Kentucky, Lexington, KY 40546-0312, United States
Laboratorio de Fisiología y Biología Molecular, Dept. Fisiología, Biología Molecular y Celular, IFIBYMECONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:Bromus setifolius; Endophyte diversity; Epichloë; Neotyphodium; Phleum alpinum; Phylogeny; genotype; grass; molecular analysis; new species; species diversity; symbiosis; Argentina; Patagonia; fungal DNA; ribosomal spacer DNA; article; biodiversity; Bromus setifolius; classification; endophyte diversity; Epichloe; Epichloë; fungus spore; genetics; growth, development and aging; isolation and purification; microbiology; molecular genetics; Neotyphodium; Phleum alpinum; phylogeny; physiology; Poaceae; symbiosis; Bromus setifolius; endophyte diversity; Epichloë; Neotyphodium; Phleum alpinum; phylogeny; Biodiversity; DNA, Fungal; DNA, Ribosomal Spacer; Epichloe; Molecular Sequence Data; Neotyphodium; Phylogeny; Poaceae; Spores, Fungal; Symbiosis
Año:2014
Volumen:106
Número:2
Página de inicio:339
Página de fin:352
DOI: http://dx.doi.org/10.3852/106.2.339
Título revista:Mycologia
Título revista abreviado:Mycologia
ISSN:00275514
CODEN:MYCOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v106_n2_p339_McCargo

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Citas:

---------- APA ----------
McCargo, P.D., Iannone, L.J., Vignale, M.V., Schardl, C.L. & Rossi, M.S. (2014) . Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia. Mycologia, 106(2), 339-352.
http://dx.doi.org/10.3852/106.2.339
---------- CHICAGO ----------
McCargo, P.D., Iannone, L.J., Vignale, M.V., Schardl, C.L., Rossi, M.S. "Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia" . Mycologia 106, no. 2 (2014) : 339-352.
http://dx.doi.org/10.3852/106.2.339
---------- MLA ----------
McCargo, P.D., Iannone, L.J., Vignale, M.V., Schardl, C.L., Rossi, M.S. "Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia" . Mycologia, vol. 106, no. 2, 2014, pp. 339-352.
http://dx.doi.org/10.3852/106.2.339
---------- VANCOUVER ----------
McCargo, P.D., Iannone, L.J., Vignale, M.V., Schardl, C.L., Rossi, M.S. Species diversity of Epichloë symbiotic with two grasses from southern Argentinean Patagonia. Mycologia. 2014;106(2):339-352.
http://dx.doi.org/10.3852/106.2.339