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Abstract:

The fungi of genus Neotyphodium are systemic, constitutive, symbionts of grasses of subfamily Pooideae. In the southern hemisphere most of these asexual endophytes are the result of the hybridization between two sexual species, Epichloe festucae and E. typhina, from the northern hemisphere. However the ancestral sexual species have not been detected in this region. Several grasses from Argentina are infected by Neotyphodium species. These endophytes are in general very similar macro-and micromorphologically and phylogenetically conform to species N tembladerae. However the Neotyphodium spp. endophytes of some hosts, Bromus auleticus and Poa spicifomis var. spiciformis, have not been included in this species. In this work we studied the incidence and characterized the diversity of Neotyphodium species in populations of the native grass Bromus auleticus from Argentina. The incidence of endophytes was 100% in all populations investigated. Two groups of endophytes were differentiated by their morphologies, growth rates, conidial ontogenies and by relative resistance to the fungicide benomyl. Phylogenetic trees inferred from tefA and tubB intron sequences indicated that both N tembladerae and the novel morphotype were hybrids of E. festucae and E. typhina, but the ancestral E. typhina genotype distinguished them. Isolates from plants that inhabit coastal dunes, xerophytic forests, savannahs and hills were similar morphologically and phylogenetically to N tembladerae, whereas the endophytes from the humid pampa plains conformed to the novel group. We propose the endophyte of Bromus auleticus from humid pampas as a new species, Neotyphodium pampeanum. © 2009 by The Mycological Society of America.

Registro:

Documento: Artículo
Título:Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America
Autor:Iannone, L.J.; Cabral, D.; Schardl, C.L.; Rossi, M.S.
Filiación:Departamento Biodiversidad y Biología Experimental, PRHIDEB-CONICET, Facultad de Ciencias Exactas y Naturaks, Universidad de Buenos Aires, Argentina
Department of Plant Pathology, University of Kentucky, Lexington, KY 40546-0312, United States
Departamento Fisiologia, Biología Molecular y Celular, WIBYME-CONICET, Facultad de Ciencias Exactas y Naturaks, Universidad de Buenos Aires, Argentina
LFBM, IFIBYME-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina, Ciudad Universitaria-Pabellon II, 2do Piso, EHA1428-Cdad. de Buenos Aires, Argentina
Palabras clave:Bromus auleticus; Endophyte diversity; Molecular phylogeny; Neotyphodium; divergence; endophyte; fungicide; fungus; genotype; grass; growth rate; hybridization; morphology; new species; ontogeny; pesticide resistance; phylogenetics; physiology; species diversity; South America; Bromus; Epichloe festucae; Fungi; Neotyphodium; Poa; Poaceae; Pooideae; elongation factor 1; fungal DNA; ribosomal spacer DNA; tubulin; Argentina; article; Bromus; classification; cytology; DNA sequence; ecosystem; fungus spore; genetic variability; genetics; isolation and purification; microbiology; Neotyphodium; phylogeny; physiology; species difference; species differentiation; symbiosis; Argentina; Bromus; DNA, Fungal; DNA, Ribosomal Spacer; Ecosystem; Genetic Speciation; Genetic Variation; Neotyphodium; Peptide Elongation Factor 1; Phylogeny; Sequence Analysis, DNA; Species Specificity; Spores, Fungal; Symbiosis; Tubulin
Año:2009
Volumen:101
Número:3
Página de inicio:340
Página de fin:351
DOI: http://dx.doi.org/10.3852/08-156
Título revista:Mycologia
Título revista abreviado:Mycologia
ISSN:00275514
CODEN:MYCOA
CAS:DNA, Fungal; DNA, Ribosomal Spacer; Peptide Elongation Factor 1; Tubulin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00275514_v101_n3_p340_Iannone

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Citas:

---------- APA ----------
Iannone, L.J., Cabral, D., Schardl, C.L. & Rossi, M.S. (2009) . Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America. Mycologia, 101(3), 340-351.
http://dx.doi.org/10.3852/08-156
---------- CHICAGO ----------
Iannone, L.J., Cabral, D., Schardl, C.L., Rossi, M.S. "Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America" . Mycologia 101, no. 3 (2009) : 340-351.
http://dx.doi.org/10.3852/08-156
---------- MLA ----------
Iannone, L.J., Cabral, D., Schardl, C.L., Rossi, M.S. "Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America" . Mycologia, vol. 101, no. 3, 2009, pp. 340-351.
http://dx.doi.org/10.3852/08-156
---------- VANCOUVER ----------
Iannone, L.J., Cabral, D., Schardl, C.L., Rossi, M.S. Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America. Mycologia. 2009;101(3):340-351.
http://dx.doi.org/10.3852/08-156