Artículo

Rossi, M.; Carrari, F.; Cabrera-Ponce, J.L.; Vázquez-Rovere, C.; Herrera-Estrella, L.; Gudesblat, G.; Iusem, N.D. "Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems" (1998) Molecular and General Genetics. 258(1-2):1-8
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Abstract:

Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To prove that the promoter region is functional in vivo, we fused it upstream of the β-glucuronidase (GUS) reporter gene. The resulting chimeric gene fusion was used for transient expression assays in papaya embryogenic calli and leaves. In addition, the same construct was used to produce transgenic tomato, papaya, tobacco, and potato plants. Asr2 upstream sequences showed promoter function in all of these systems. Under the experimental conditions tested, ABA stimulated GUS expression in papaya and tobacco, but not in tomato and potato systems.

Registro:

Documento: Artículo
Título:Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems
Autor:Rossi, M.; Carrari, F.; Cabrera-Ponce, J.L.; Vázquez-Rovere, C.; Herrera-Estrella, L.; Gudesblat, G.; Iusem, N.D.
Filiación:Lab. de Fisiol. y Biologia Molecular, Depto. de Ciencias Biológicas, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina
Instituto de Biotecnologia, CICV, Inst. Nac. de Tecn. Agropecuaria, CC 1708 Morón, Argentina
Ctro. Invest. Estud. Avanzados I., Unidad Irapuato, Mexico
Palabras clave:Abscisic acid (ABA); Asr genes; Asr2; Drought stress; Tomato; abscisic acid; beta glucuronidase; article; gene expression; gene fusion; multigene family; nonhuman; priority journal; promoter region; reporter gene; tomato; transcription regulation; Carica papaya; Embryophyta; Lycopersicon esculentum; Nicotiana tabacum; Solanum tuberosum
Año:1998
Volumen:258
Número:1-2
Página de inicio:1
Página de fin:8
DOI: http://dx.doi.org/10.1007/s004380050700
Título revista:Molecular and General Genetics
Título revista abreviado:Mol. Gen. Genet.
ISSN:00268925
CODEN:MGGEA
CAS:abscisic acid, 21293-29-8; beta glucuronidase, 9001-45-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00268925_v258_n1-2_p1_Rossi

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Citas:

---------- APA ----------
Rossi, M., Carrari, F., Cabrera-Ponce, J.L., Vázquez-Rovere, C., Herrera-Estrella, L., Gudesblat, G. & Iusem, N.D. (1998) . Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems. Molecular and General Genetics, 258(1-2), 1-8.
http://dx.doi.org/10.1007/s004380050700
---------- CHICAGO ----------
Rossi, M., Carrari, F., Cabrera-Ponce, J.L., Vázquez-Rovere, C., Herrera-Estrella, L., Gudesblat, G., et al. "Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems" . Molecular and General Genetics 258, no. 1-2 (1998) : 1-8.
http://dx.doi.org/10.1007/s004380050700
---------- MLA ----------
Rossi, M., Carrari, F., Cabrera-Ponce, J.L., Vázquez-Rovere, C., Herrera-Estrella, L., Gudesblat, G., et al. "Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems" . Molecular and General Genetics, vol. 258, no. 1-2, 1998, pp. 1-8.
http://dx.doi.org/10.1007/s004380050700
---------- VANCOUVER ----------
Rossi, M., Carrari, F., Cabrera-Ponce, J.L., Vázquez-Rovere, C., Herrera-Estrella, L., Gudesblat, G., et al. Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems. Mol. Gen. Genet. 1998;258(1-2):1-8.
http://dx.doi.org/10.1007/s004380050700