Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Escherichia coli, cultured on minimal medium and deprived of its required amino-acids, was induced for lac genes transcription. After inducer removal and restoration of growth, β-galactosidase synthesis was measured. Two different kinetics of enzyme synthesis were observed depending on the starvation conditions employed during the induction period: 1. β-galactosidase synthesis was immediately obtained and a plateau was reached, in 20 min after restoration of growth, when cells had been induced during deprivation of amino-acids and carbon source. 2. β-galactosidase displayed an unusually long rate of synthesis and plateau was not reached before two doubling times, when cells had been induced during the deprivation of the sole amino-acids. The latter result points out a problem of messenger stability during those long translation kinetics and led us to study the behaviour of strains carrying lac genetic determinants on different replicative structures: chromosomic and plasmidic. In those two situations, induction of lac messenger RNA was obtained and ratify our previous observations. However, their translation kinetics suggest a DNA linkage of this induced messenger. © 1977 Springer-Verlag.

Registro:

Documento: Artículo
Título:Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation
Autor:de Rivas, C.S.; Mendez, B.S.
Filiación:Centro de Investigaciones Microbiologicas, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Nuñez, Buenos Aires, Argentina
Palabras clave:amino acid; beta galactosidase; lactose; messenger rna; escherichia coli; in vitro study; microorganism; operon; rna synthesis; starvation; theoretical study; Chromosomes, Bacterial; Comparative Study; Escherichia coli; F Factor; Genes; Kinetics; Lactose; RNA, Messenger; Translation, Genetic
Año:1977
Volumen:156
Número:2
Página de inicio:229
Página de fin:232
DOI: http://dx.doi.org/10.1007/BF00283496
Título revista:MGG Molecular & General Genetics
Título revista abreviado:Molec. Gen. Genet.
ISSN:00268925
CODEN:MGGEA
CAS:amino acid, 65072-01-7; lactose, 10039-26-6, 16984-38-6, 63-42-3, 64044-51-5; F Factor; Lactose, 63-42-3; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00268925_v156_n2_p229_deRivas

Referencias:

  • Billen, D., Hewitt, R., Influence of starvation for methionine and other aminoacids on subsequent bacterial deoxyribonucleic acid replication (1966) J. Bact., 92, pp. 609-617
  • Copeland, J.C., Phillips, S.J., Mao, M.W.H.: Effect of aminoacid starvation on chromosome replication in Bacillus subtilis. Microbiology, ed. by D. Schlessinger, pp. 70–82 (1976); Craig, E., Schlessinger, D., Gurgo, C., Synthesis, no synthesis, or synthesis and degradation of ribosomal ribonucleic acid in various Escherichia coli strains starved for an amino acid (1972) J. Bact., 111, pp. 66-72
  • Frame, R., Bishop, J.O., The number of sex-factors per chromosome in Escherichia coli (1971) Biochem. J., 121, pp. 93-103
  • Gierer, L., Nascent ribosomal and messenger RNA in DNA-membrane-complexes of Escherichia coli (1973) MGG Molecular & General Genetics, 125, pp. 173-181
  • Gurgo, C., Craig, E., Schlessinger, D., Afolayan, A., Polyribosome metabolism in Escherichia coli starved for an amino-acid (1971) J. molec. Biol., 62, pp. 525-535
  • Jacob, F., Monod, J., Genetic regulatory mechanisms in the synthesis of proteins (1961) J. molec. Biol., 3, pp. 318-356
  • Lark, K.K., Repko, T., Hoffman, E.J., The effect of amino acid deprivation on subsequent deoxyribonucleic acid replication (1963) Biochim. biophys. Acta (Amst.), 76, pp. 9-29
  • Lazzarini, R.A., Dalberg, A.E., The control of ribonucleic acid synthesis during amino acid deprivation in Escherichia coli (1971) J. biol. Chem., 246, pp. 420-429
  • Lazzarini, R.A., Winslow, R.M., The regulation of RNA synthesis during growth rate transitions and amino acid deprivation in E. coli (1970) Cold Spring Harbor Symposia on Quantitative Biology, 35, pp. 383-390
  • Leal, J., Effect of glucose starvation on the expression of transferred tox genes in Escherichia coli K12 zygotes (1976) Molec. gen. Genet., 147, pp. 53-58
  • Levy, S.B., Very stable prokaryotic messenger RNA in chromosomeless Escherichia coli minicells (1975) Proc. nat. Acad. Sci. (Wash.), 72, pp. 2900-2904
  • Miller, J.H., (1972) Experiments in Molecular Genetics, , Cold Spring Harbor Laboratory, New York
  • Nakada, D., Magasanik, B., The roles of inducer and catabolite repressor in the synthesis of β-galactosidase by Escherichia coli (1964) J. molec. Biol., 8, pp. 105-127
  • Pritchard, R.H., Chandler, M.G., Collins, J., Independance of F replication and chromosome replication in Escherichia coli (1975) Molec. gen. Genet., 138, pp. 143-155
  • Puga, A., Borras, M.T., Tessman, E.S., Tessman, I., Difference between functional and structural integrity of messenger RNA (1973) Proceedings of the National Academy of Sciences, 70, pp. 2171-2175
  • Richardson, J.P., Attachment of nascent RNA molecules to superhelical DNA (1975) J. molec. Biol., 98, pp. 565-579
  • Sanchez de Rivas, C., Mendez, B.S., Catabolite translational effects on the lac messenger RNA of Escherichia coli K12 (1976) Molec. gen. Genet., 148, pp. 99-104
  • Schlessinger, D., Apririon, D., Escherichia coli ribosomes: recent developments (1969) Ann. Rev. Microbiol., 23, pp. 387-426
  • Summer, W.C., The process of infection with coliphage T<inf>7</inf>. IV. Stability of RNA in bacteriophage-infected cells (1970) J. molec. Biol., 50, pp. 671-678

Citas:

---------- APA ----------
de Rivas, C.S. & Mendez, B.S. (1977) . Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation. MGG Molecular & General Genetics, 156(2), 229-232.
http://dx.doi.org/10.1007/BF00283496
---------- CHICAGO ----------
de Rivas, C.S., Mendez, B.S. "Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation" . MGG Molecular & General Genetics 156, no. 2 (1977) : 229-232.
http://dx.doi.org/10.1007/BF00283496
---------- MLA ----------
de Rivas, C.S., Mendez, B.S. "Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation" . MGG Molecular & General Genetics, vol. 156, no. 2, 1977, pp. 229-232.
http://dx.doi.org/10.1007/BF00283496
---------- VANCOUVER ----------
de Rivas, C.S., Mendez, B.S. Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation. Molec. Gen. Genet. 1977;156(2):229-232.
http://dx.doi.org/10.1007/BF00283496