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Abstract:

The transcriptional and translational events occurring during the induction of the lac operon, were separated by blocking the translational step, either by aminoacid starvation or by addition of chloramphenicol. It was found that the carbon source used during the subsequent translation, affected the rate of β-galactosidase synthesis. A decoordination effect on the production of enzymes of the lac system was also observed in high catabolite repression media, as well as in nitrogen limiting conditions. These findings suggested a similarity with the polarity phenomenon. In order to test this similarity, polarity suppressors of a Z- polar mutant were isolated. In one of these mutants, probably suA like, no carbon source effect was observed during the translational step. The induction kinetics in different media, after distinct pregrowth conditions, supported the idea that this mutant could be considered catabolite repression resistant only in certain restrictive conditions. © 1976 Springer-Verlag.

Registro:

Documento: Artículo
Título:Catabolite translational effects on the lac messenger RNA of Escherichia coli K12
Autor:de Rivas, C.S.; Méndez, B.S.
Filiación:Centro de Investigaciones Microbiologicas, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Nuñez, Buenos Aires, Argentina
Año:1976
Volumen:148
Número:1
Página de inicio:99
Página de fin:104
DOI: http://dx.doi.org/10.1007/BF00268550
Título revista:MGG Molecular & General Genetics
Título revista abreviado:Molec. Gen. Genet.
ISSN:00268925
CODEN:MGGEA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00268925_v148_n1_p99_deRivas

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Citas:

---------- APA ----------
de Rivas, C.S. & Méndez, B.S. (1976) . Catabolite translational effects on the lac messenger RNA of Escherichia coli K12. MGG Molecular & General Genetics, 148(1), 99-104.
http://dx.doi.org/10.1007/BF00268550
---------- CHICAGO ----------
de Rivas, C.S., Méndez, B.S. "Catabolite translational effects on the lac messenger RNA of Escherichia coli K12" . MGG Molecular & General Genetics 148, no. 1 (1976) : 99-104.
http://dx.doi.org/10.1007/BF00268550
---------- MLA ----------
de Rivas, C.S., Méndez, B.S. "Catabolite translational effects on the lac messenger RNA of Escherichia coli K12" . MGG Molecular & General Genetics, vol. 148, no. 1, 1976, pp. 99-104.
http://dx.doi.org/10.1007/BF00268550
---------- VANCOUVER ----------
de Rivas, C.S., Méndez, B.S. Catabolite translational effects on the lac messenger RNA of Escherichia coli K12. Molec. Gen. Genet. 1976;148(1):99-104.
http://dx.doi.org/10.1007/BF00268550