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Abstract:

In this paper, we report the study of proteinaceous binders in samples from a South American Colonial 18th century painting using two complementary mass spectrometry techniques. Before restoration, seven micro-samples were extracted from representative colors of one of the mural paintings located inside the church of Our Lady of Copacabana de Andamarca built in 1723 in Bolivia. Previous analysis by gas chromatography (GC) of the amino acid derivatives of the extracted protein fraction of three of the samples, suggested the presence of animal glue and egg. In this work, we introduce a methodology that combines a protein extraction procedure with a typical treatment for analysis of proteins as tryptic peptides by mass spectrometry. For this study, we applied in the first stage, MALDI-TOF/MS and then, LC-ESI/MS (Orbitrap), an ultrahigh resolution mass spectrometry, to achieve more reliability in the identification of the protein binders. By LC-ESI/MS (Orbitrap), we detected several peptides from egg white proteins, particularly ovalbumin, ovotransferrin and lysozyme, and egg yolk proteins, vitellogenin-2 and apolipoprotein B, with high confidence peptides each. In all the samples, the presence of collagen from animal provenance was established by MALDI-TOF/MS and LC-ESI/MS (Orbitrap). To our knowledge, this is the first time that protein binders in Andean paintings are identified without ambiguity by MALDI-TOF/MS and LC-ESI/MS (Orbitrap) mass spectrometry using a proteomic approach. © 2018 Elsevier B.V.

Registro:

Documento: Artículo
Título:Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)
Autor:Levy, I.K.; Neme Tauil, R.; Valacco, M.P.; Moreno, S.; Siracusano, G.; Maier, M.S.
Filiación:UMYMFOR-CONICET and Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina
CONICET, Godoy Cruz 2290, Ciudad Autónoma de Buenos Aires, C1425FQB, Argentina
Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina
Centro de Investigación en Arte, Materia y Cultura (IIAC), Universidad Nacional de Tres de Febrero, Avenida Antártida Argentina 1355, Ciudad Autónoma de Buenos Aires, C1104ACA, Argentina
Palabras clave:Colonial mural paintings; Egg tempera technique; LC-ESI/MS (Orbitrap); MALDI-TOF/MS; Proteinaceous binders; Proteomic tools
Año:2018
Volumen:143
Página de inicio:457
Página de fin:466
DOI: http://dx.doi.org/10.1016/j.microc.2018.07.030
Título revista:Microchemical Journal
Título revista abreviado:Microchem. J.
ISSN:0026265X
CODEN:MICJA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0026265X_v143_n_p457_Levy

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Citas:

---------- APA ----------
Levy, I.K., Neme Tauil, R., Valacco, M.P., Moreno, S., Siracusano, G. & Maier, M.S. (2018) . Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap). Microchemical Journal, 143, 457-466.
http://dx.doi.org/10.1016/j.microc.2018.07.030
---------- CHICAGO ----------
Levy, I.K., Neme Tauil, R., Valacco, M.P., Moreno, S., Siracusano, G., Maier, M.S. "Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)" . Microchemical Journal 143 (2018) : 457-466.
http://dx.doi.org/10.1016/j.microc.2018.07.030
---------- MLA ----------
Levy, I.K., Neme Tauil, R., Valacco, M.P., Moreno, S., Siracusano, G., Maier, M.S. "Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)" . Microchemical Journal, vol. 143, 2018, pp. 457-466.
http://dx.doi.org/10.1016/j.microc.2018.07.030
---------- VANCOUVER ----------
Levy, I.K., Neme Tauil, R., Valacco, M.P., Moreno, S., Siracusano, G., Maier, M.S. Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap). Microchem. J. 2018;143:457-466.
http://dx.doi.org/10.1016/j.microc.2018.07.030