Artículo
Beauquis, J.; Vinuesa, Á.; Pomilio, C.; Pavía, P.; Saravia, F. "Hippocampal and cognitive alterations precede amyloid deposition in a mouse model of al- zheimer’s disease " (2014) Medicina (Argentina). 74(4):282-286
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Abstract:
Although there is strong evidence about neuronal and glial disturbances at advanced stages of Alzheimer’s disease, less attention has been directed to early, pre-amyloid changes that could contribute to the progression of the disease. We evaluated neuronal and glial morphological changes and behavioral disturbances in PDAPP-J20 transgenic (Tg) mice, carrying mutated human APP gene (amyloid precursor protein), at 5 months of age, before brain amyloid deposition occurs. Using NeuN immunohistochemistry we found decreased numbers of pyramidal and granular neurons in the hippocampus associated with a reduction of hippocampal volume in Tg mice compared with controls. Neurogenesis was impaired, evidenced by means of DCX immunohistochemistry in the dentate gyrus. In the CA3 region we found a decreased density of synaptophysin, suggesting synaptic disturbance, but no changes were found in CA1 synaptic spine density. Using confocal microscopy we observed decreased number and cell complexity of GFAP+ astrocytes, indicating potential glial atrophy. Cognitive impairment (novel location recognition test) and increased anxiety (open field) were detected in Tg mice, associated with more c-Fos+ nuclei in the amygdala, possibly indicating a role for emotionality in early stages of the disease. The study of early alterations in the course of amyloid pathology could contribute to the development of diagnostic and preventive strategies. © 2014, MEDICINA (Buenos Aires). All Rights Reserved.
Registro:
| Documento: | Artículo |
| Título: | Hippocampal and cognitive alterations precede amyloid deposition in a mouse model of al- zheimer’s disease |
| Autor: | Beauquis, J.; Vinuesa, Á.; Pomilio, C.; Pavía, P.; Saravia, F. |
| Filiación: | Laboratorio de Neurobiología del Envejecimiento, Instituto de Biología y Medicina Experimental, CONICET, Argentina Laboratorio de Neurobiología, Departamento de Química Biológica, Universidad de Buenos Aires, Argentina |
| Palabras clave: | Alzheimer’s disease; Glia; Hippocampus; Memory; Neurogenesis; Transgenic mice; amyloid; brain protein; protein precursor; amyloid precursor protein; APP protein, human; synaptophysin; Alzheimer disease; amygdaloid nucleus; animal experiment; animal model; animal tissue; Article; behavior disorder; cognitive defect; controlled study; dentate gyrus; emotionality; hippocampus; immunohistochemistry; mouse; nerve cell; nonhuman; oncogene c fos; Alzheimer disease; amyloid plaque; animal; anxiety disorder; astrocyte; C57BL mouse; disease course; disease model; genetics; hippocampus; human; isolation and purification; metabolism; mild cognitive impairment; nervous system development; pathology; physiology; transgenic mouse; Alzheimer Disease; Amyloid beta-Protein Precursor; Animals; Anxiety Disorders; Astrocytes; Dentate Gyrus; Disease Models, Animal; Disease Progression; Hippocampus; Humans; Mice, Inbred C57BL; Mice, Transgenic; Mild Cognitive Impairment; Neurogenesis; Neurons; Plaque, Amyloid; Synaptophysin |
| Año: | 2014 |
| Volumen: | 74 |
| Número: | 4 |
| Página de inicio: | 282 |
| Página de fin: | 286 |
| Título revista: | Medicina (Argentina) |
| Título revista abreviado: | Medicina |
| ISSN: | 00257680 |
| CODEN: | MEDCA |
| CAS: | amyloid, 11061-24-8; Amyloid beta-Protein Precursor; APP protein, human; Synaptophysin |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v74_n4_p282_Beauquis |
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Citas:
---------- APA ----------
Beauquis, J., Vinuesa, Á., Pomilio, C., Pavía, P. & Saravia, F. (2014) . Hippocampal and cognitive alterations precede amyloid deposition in a mouse model of al- zheimer’s disease . Medicina (Argentina), 74(4), 282-286.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v74_n4_p282_Beauquis [ ]
---------- CHICAGO ----------
Beauquis, J., Vinuesa, Á., Pomilio, C., Pavía, P., Saravia, F. "Hippocampal and cognitive alterations precede amyloid deposition in a mouse model of al- zheimer’s disease " . Medicina (Argentina) 74, no. 4 (2014) : 282-286.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v74_n4_p282_Beauquis [ ]
---------- MLA ----------
Beauquis, J., Vinuesa, Á., Pomilio, C., Pavía, P., Saravia, F. "Hippocampal and cognitive alterations precede amyloid deposition in a mouse model of al- zheimer’s disease " . Medicina (Argentina), vol. 74, no. 4, 2014, pp. 282-286.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v74_n4_p282_Beauquis [ ]
---------- VANCOUVER ----------
Beauquis, J., Vinuesa, Á., Pomilio, C., Pavía, P., Saravia, F. Hippocampal and cognitive alterations precede amyloid deposition in a mouse model of al- zheimer’s disease . Medicina. 2014;74(4):282-286.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v74_n4_p282_Beauquis [ ]
