Artículo
Nojek, I.M.; Werbajh, S.E.; Colo, G.P.; Rubio, F.M.; Franco, L.D.; Nahmod, V.E.; Costas, M.A. "Different enzymatic activities recruitment by specific domains of TIF2 are involved in NF-κB transactivation " (2004) Medicina. 64(2):135-138
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Abstract:
We have previously shown that nuclear receptor coactivator overexpression significantly enhanced NF-κB activity in a dose response manner. We studied the mechanism by which TIF2 regulates NF-κB activity. We determined that: 1) the p38 specific inhibitor reduces 50% NF-κB transcriptional activity, even in cells that overexpress distinct TIF2 deletions; 2) there is a physical interaction between TIF2 and p38 and RelA determined through in vitro translated protein bindind assays; 3) TIF2 is a p38 substrate; 4) there is a physical interaction between TIF2 and IKK in TNF-α 20 ng/ml stimulated or not HEK 293 cell protein extract, and IκB only in basal conditions, determined by binding pull down assays. This NF-κ B complex regulates its activity and targets gene expression in a determined physiologic context depending on the coactivator complex content.
Registro:
| Documento: | Artículo |
| Título: | Different enzymatic activities recruitment by specific domains of TIF2 are involved in NF-κB transactivation |
| Autor: | Nojek, I.M.; Werbajh, S.E.; Colo, G.P.; Rubio, F.M.; Franco, L.D.; Nahmod, V.E.; Costas, M.A. |
| Filiación: | Inst. de Invest. Med. Alfredo Lanari, Universidad de Buenos Aires, Combatientes de Malvinas 3150, 1427 Buenos Aires, Argentina Fundación Instituto Leloir, Universidad de Buenos Aires, Buenos Aires, Argentina Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina |
| Palabras clave: | MAPK; NF-κB; Nuclear receptor coactivators; TIF2; cell protein; I kappa B; immunoglobulin enhancer binding protein; mitogen activated protein kinase; nuclear receptor coactivator 2; synaptophysin; transcription factor RelA; tumor necrosis factor alpha; immunoglobulin enhancer binding protein; mitogen activated protein kinase p38; NCOA2 protein, human; nuclear receptor coactivator 2; transcription factor; article; dose response; enzyme activity; gene overexpression; human; human cell; protein binding; protein determination; protein expression; protein interaction; transactivation; transcription regulation; cell nucleus; cytoplasm; drug antagonism; enzyme activation; metabolism; phosphorylation; physiology; Cell Nucleus; Cytoplasm; Enzyme Activation; Humans; NF-kappa B; Nuclear Receptor Coactivator 2; p38 Mitogen-Activated Protein Kinases; Phosphorylation; Trans-Activation (Genetics); Transcription Factors |
| Año: | 2004 |
| Volumen: | 64 |
| Número: | 2 |
| Página de inicio: | 135 |
| Página de fin: | 138 |
| Título revista: | Medicina |
| Título revista abreviado: | Medicina (Argentina) |
| ISSN: | 00257680 |
| CODEN: | MEDCA |
| CAS: | mitogen activated protein kinase, 142243-02-5; NCOA2 protein, human; NF-kappa B; Nuclear Receptor Coactivator 2; p38 Mitogen-Activated Protein Kinases, EC 2.7.1.37; Transcription Factors |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v64_n2_p135_Nojek |
Referencias:
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Citas:
---------- APA ----------
Nojek, I.M., Werbajh, S.E., Colo, G.P., Rubio, F.M., Franco, L.D., Nahmod, V.E. & Costas, M.A. (2004) . Different enzymatic activities recruitment by specific domains of TIF2 are involved in NF-κB transactivation . Medicina, 64(2), 135-138.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v64_n2_p135_Nojek [ ]
---------- CHICAGO ----------
Nojek, I.M., Werbajh, S.E., Colo, G.P., Rubio, F.M., Franco, L.D., Nahmod, V.E., et al. "Different enzymatic activities recruitment by specific domains of TIF2 are involved in NF-κB transactivation " . Medicina 64, no. 2 (2004) : 135-138.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v64_n2_p135_Nojek [ ]
---------- MLA ----------
Nojek, I.M., Werbajh, S.E., Colo, G.P., Rubio, F.M., Franco, L.D., Nahmod, V.E., et al. "Different enzymatic activities recruitment by specific domains of TIF2 are involved in NF-κB transactivation " . Medicina, vol. 64, no. 2, 2004, pp. 135-138.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v64_n2_p135_Nojek [ ]
---------- VANCOUVER ----------
Nojek, I.M., Werbajh, S.E., Colo, G.P., Rubio, F.M., Franco, L.D., Nahmod, V.E., et al. Different enzymatic activities recruitment by specific domains of TIF2 are involved in NF-κB transactivation . Medicina (Argentina). 2004;64(2):135-138.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v64_n2_p135_Nojek [ ]
