Artículo
Giacomini, D.; Paez-Pereda, M.; Refojo, D.; Nagashima, A.C.; Chervin, A.; Goldberg, V.; Arzt, E. "New mechanisms involved in the pathogenesis of pituitary adenomas " (2003) Medicina. 63(2):147-150
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Abstract:
We studied Smad-4dn tumors generated from lactosomatotrophic GH3 cells stably transfected with a dominant negative form of Smad-4 (a bone morphogenetic protein-4, BMP-4, signal co-transducer) which had reduced tumorigenicity in nude mice, but had showed a late increase in tumor size. We found that they had lost in vivo the expression of Smad-4dn and had recovered c-Myc expression. In accordance, BMP-4 is overexpressed and stimulates the expression of c-Myc in human prolactinomas, but not in other human pituitary adenomas or normal pituitary. In adittion ICI 182,780 inhibited BMP-4 stimulated c-Myc expression and BMP-4 and 17β-estradiol in combination had an additive effect on GH3 cell proliferation. Their action was inhibited by blocking BMP-4 with ICI 182,780 or 17β-estradiol with Smad-4dn. Furthermore, co-immunoprecipitation studies demonstrate that Smad-4 physically interacts with the ERα/ERβ. We show for the first time the role of BMP-4 in prolactinoma pathogenesis, involving a functional cross-talk BMP-4/E2.
Registro:
| Documento: | Artículo |
| Título: | New mechanisms involved in the pathogenesis of pituitary adenomas |
| Autor: | Giacomini, D.; Paez-Pereda, M.; Refojo, D.; Nagashima, A.C.; Chervin, A.; Goldberg, V.; Arzt, E. |
| Filiación: | Depto. de Fisiol. Biol. Molec./Cel., Facultad Ciencias Exactas Naturales, Universidad de Buenos Aires, Pabellón II, 1428 Buenos Aires, Argentina Inst. de Invest. Med. Alfredo Lanari, Facultad de Medicina, Universidad de Buneos Aires, Pabellón II, 1428 Buenos Aires, Argentina Hospital Santa Lucía, Buenos Aires, Argentina |
| Palabras clave: | c-Myc; Proclatinoma; bone morphogenetic protein 4; estradiol; estrogen receptor alpha; estrogen receptor beta; fulvestrant; Myc protein; Smad protein; animal experiment; animal model; article; carcinogenicity; controlled study; gene overexpression; genetic transfection; hypophysis adenoma; immunoprecipitation; in vivo study; mouse; nonhuman; nude mouse; pathogenesis; prolactinoma; protein expression; protein function; protein protein interaction; regulatory mechanism; somatic cell; tumor volume; Animals; Bone Morphogenetic Proteins; Cell Division; DNA-Binding Proteins; Humans; Mice; Mice, Nude; Pituitary Neoplasms; Prolactinoma; Proto-Oncogene Proteins c-myc; Signal Transduction; Smad4 Protein; Trans-Activators |
| Año: | 2003 |
| Volumen: | 63 |
| Número: | 2 |
| Página de inicio: | 147 |
| Página de fin: | 150 |
| Título revista: | Medicina |
| Título revista abreviado: | Medicina (Argentina) |
| ISSN: | 00257680 |
| CODEN: | MEDCA |
| CAS: | estradiol, 50-28-2; fulvestrant, 129453-61-8; Smad protein, 62395-38-4; bone morphogenetic protein 4; Bone Morphogenetic Proteins; DNA-Binding Proteins; Proto-Oncogene Proteins c-myc; Smad4 Protein; SMAD4 protein, human; Smad4 protein, mouse; Trans-Activators |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v63_n2_p147_Giacomini |
Referencias:
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Citas:
---------- APA ----------
Giacomini, D., Paez-Pereda, M., Refojo, D., Nagashima, A.C., Chervin, A., Goldberg, V. & Arzt, E. (2003) . New mechanisms involved in the pathogenesis of pituitary adenomas . Medicina, 63(2), 147-150.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v63_n2_p147_Giacomini [ ]
---------- CHICAGO ----------
Giacomini, D., Paez-Pereda, M., Refojo, D., Nagashima, A.C., Chervin, A., Goldberg, V., et al. "New mechanisms involved in the pathogenesis of pituitary adenomas " . Medicina 63, no. 2 (2003) : 147-150.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v63_n2_p147_Giacomini [ ]
---------- MLA ----------
Giacomini, D., Paez-Pereda, M., Refojo, D., Nagashima, A.C., Chervin, A., Goldberg, V., et al. "New mechanisms involved in the pathogenesis of pituitary adenomas " . Medicina, vol. 63, no. 2, 2003, pp. 147-150.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v63_n2_p147_Giacomini [ ]
---------- VANCOUVER ----------
Giacomini, D., Paez-Pereda, M., Refojo, D., Nagashima, A.C., Chervin, A., Goldberg, V., et al. New mechanisms involved in the pathogenesis of pituitary adenomas . Medicina (Argentina). 2003;63(2):147-150.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v63_n2_p147_Giacomini [ ]
