Artículo
Abstract:
This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor mRNA, play specific roles during follicular development. In particular, we analyzed the presence of the ED-I region, which is absent in the plasma form. Analysis of FN levels in follicular fluids corresponding to different stages of development of bovine follicles revealed marked changes in the concentrations of ED-I+FN whereas total FN levels remained relatively constant. A negative correlation (P < 0.001) was detected between ED-I+FN and estradiol levels. This steroid was without effect on the alternative splicing of FN in primary cultures of bovine granulosa cells. However, cAMP produced a marked decrease in the incorporation of the ED-I region. In contrast, transforming growth factor beta (TGF-β) elicited both a stimulation on overall FN synthesis and an increase in the inclusion of ED-I. This effect was evident at the protein level (Western blots) and also in the mRNAs (Northern blots). A peptide corresponding to the ED-I region stimulated DNA synthesis in a bovine granulosa cell line (BGC-1) whereas the peptide corresponding to the flanking sequences was without effect. Data presented herein suggest a novel form of regulation by which changes in the primary structure of FN may mediate some of the effects of gonadotropin and intraovarian factors during follicular development.
Registro:
| Documento: | Artículo |
| Título: | Role of different forms of fibronectin in in vitro bovine follicular development |
| Autor: | Colman Lerner, A.A.; Fischman, M.L.; Lanuza, G.; Cramer, P.; Kornblihtt, A.; Barañao, J.L. |
| Filiación: | Inst. de Biol. y Med. Experimental, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina Inst. Invest. Genet. y Biol. Molec., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | fibronectin; animal; article; cattle; chemistry; female; growth, development and aging; ovary follicle; ovary follicle fluid; physiology; Animals; Cattle; Female; Fibronectins; Follicular Fluid; Ovarian Follicle |
| Año: | 1997 |
| Volumen: | 57 |
| Número: | 3 |
| Página de inicio: | 332 |
| Página de fin: | 336 |
| Título revista: | Medicina |
| Título revista abreviado: | Medicina (Argentina) |
| ISSN: | 00257680 |
| CODEN: | MEDCA |
| CAS: | Fibronectins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n3_p332_ColmanLerner |
Referencias:
- Aten, R.F., Kolodecik, T.R., Behrman, H.R., A cell adhesion receptor antiserum abolishes, whereas laminin and fibronectin glycoprotein components of extracellular matrix promote, luteinization of cultured rat granulosa cells (1995) Endocrinology, 136, pp. 1753-1758
- Kornblihtt, A.R., Pesce, C.G., Alonso, C.R., The fibronectin gene as a model for splicing and transcription studies (1996) FASEB J, 10, pp. 248-257
- Bernath, V.A., Muro, A.F., Vitullo, A.D., Bley, M.A., Baranao, J.L., Kornblihtt, A.R., Cyclic AMP inhibit fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation (1990) J Biol Chem, 265, pp. 18219-18226
- Colman Lerner, A.A., Baranao, J.L., Regulation of fibronectin production in a bovine granulosa cell line: Effects of transforming growth factor b and evidence of proteolytic processing (1995) 77th Annual Meeting of the Endocrine Society, p. 145
- Mulheron, G.W., Schamberg, D.W., The Intraovarian Transforming Growth Factor System (1993) The Ovary, pp. 337-361. , Adashi EY, Leung PCK (eds) New York: Raven Press, Ltd
- Jarnagin, W.R., Rockey, D.C., Koteliansky, V.E., Wang, S., Bissell, D.M., Expression of variant fibronectins in wound healing: Cellular source and biological activity of the EIIIA segment in rat hepatic fibrogenesis (1994) J Cell Biol, 127, pp. 2037-2048
Citas:
---------- APA ----------
Colman Lerner, A.A., Fischman, M.L., Lanuza, G., Cramer, P., Kornblihtt, A. & Barañao, J.L. (1997) . Role of different forms of fibronectin in in vitro bovine follicular development . Medicina, 57(3), 332-336.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n3_p332_ColmanLerner [ ]
---------- CHICAGO ----------
Colman Lerner, A.A., Fischman, M.L., Lanuza, G., Cramer, P., Kornblihtt, A., Barañao, J.L. "Role of different forms of fibronectin in in vitro bovine follicular development " . Medicina 57, no. 3 (1997) : 332-336.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n3_p332_ColmanLerner [ ]
---------- MLA ----------
Colman Lerner, A.A., Fischman, M.L., Lanuza, G., Cramer, P., Kornblihtt, A., Barañao, J.L. "Role of different forms of fibronectin in in vitro bovine follicular development " . Medicina, vol. 57, no. 3, 1997, pp. 332-336.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n3_p332_ColmanLerner [ ]
---------- VANCOUVER ----------
Colman Lerner, A.A., Fischman, M.L., Lanuza, G., Cramer, P., Kornblihtt, A., Barañao, J.L. Role of different forms of fibronectin in in vitro bovine follicular development . Medicina (Argentina). 1997;57(3):332-336.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00257680_v57_n3_p332_ColmanLerner [ ]
