Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Lipid and fatty acid (FA) composition and selected oxidative stress parameters of freshwater clams (Dipolodon chilensis), from a sewage-polluted (SMA) and a clean site, were compared. Trophic markers FA were analyzed in clams and sediment. Saturated FA (SAFA), and bacteria and sewage markers were abundant in SMA sediments, while diatom markers were 50% lower. Proportions of SAFA, branched FA, 20:5. n. 3 (EPA) and 22:6. n. 3 (DHA) were higher in SMA clams. Chronic exposure of D. chilensis to increasing eutrophication affected its lipid and FA composition. The increase in EPA and DHA proportions could be an adaptive response, which increases stress resistance but could also lead to higher susceptibility to lipid peroxidation TBARS, lipofuscins (20-fold) and GSH concentrations were higher in SMA clams. FA markers indicated terrestrial plant detritus and bacteria are important items in D. chilensis diet. Anthropogenic input in their food could be traced using specific FA as trophic markers. © 2013 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Effects of sewage discharges on lipid and fatty acid composition of the Patagonian bivalve Diplodon chilensis
Autor:Rocchetta, I.; Pasquevich, M.Y.; Heras, H.; Ríos de Molina, M.D.C.; Luquet, C.M.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, INQUIBICEN-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 1428 Buenos Aires, Argentina
Instituto de Investigaciones Bioquímicas de la Plata (INIBIOLP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata - CONICET CCT La Plata, Av. 60 y 120, 1900 La Plata, Argentina
Laboratorio de Ecotoxicología Acuática, INIBIOMA (CONICET-UNCo). CEAN, Junín de los Andes, ruta prov. 61 km 3. 8371, Neuquén, Argentina
Palabras clave:Domestic effluents; Fatty acid markers; Freshwater bivalve; Lipofuscins; Oxidative stress; Pollution; Domestic effluents; Fatty acid markers; Freshwater bivalve; Lipofuscins; Oxidative stress; Pollution; Animals; Biological Markers; Bivalvia; Environmental Monitoring; Fatty Acids; Lipid Peroxidation; Sewage; Thiobarbituric Acid Reactive Substances; Water Pollutants
Año:2014
Volumen:79
Número:1-2
Página de inicio:211
Página de fin:219
DOI: http://dx.doi.org/10.1016/j.marpolbul.2013.12.011
Título revista:Marine Pollution Bulletin
Título revista abreviado:Mar. Pollut. Bull.
ISSN:0025326X
CODEN:MPNBA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0025326X_v79_n1-2_p211_Rocchetta

Referencias:

  • Abdulkadir, S., Tsuchiya, M., One-step method for quantitative and qualitative analysis of fatty acids in marine animal samples (2008) J. Exp. Mar. Biol. Ecol., 354, pp. 1-8
  • Acevedo, S., Arias, N., Crego, P., Diehl, P., Flores, V., Funes, F., Ladio, A., Renner, M., (1993), Analisis del contenido intestinal de Diplodon chilensis. Trabajo especial. Catedra de Invertebrados A. Biblioteca Parasitologia C.R.U.B. Universidad del Comahue, Bariloche, Argentina; Ackman, R.G., Heras, H., Recent applications of Iatroscan TLC-FID methodology (1997) New Techniques and Applications in Lipid Analysis, pp. 325-340. , AOCS Press, Champaign, R.E. McDonald, M.M. Mossoba (Eds.)
  • Aldini, G., Dalle-Donne, I., Facino, R.M., Milzani, A., Carini, M., Intervention strategies to inhibit protein carbonylation by lipoxidation-derived reactive carbonyls (2007) Med. Res. Rev., 27, pp. 817-868
  • Alfaro, A.C., Thomas, F., Sergent, L., Duxbury, M., Identification of trophic interactions within an estuarine food web (northern New Zealand) using fatty acid biomarkers and stable isotopes (2006) Estuar. Coast. Shelf Sci., 70, pp. 271-286
  • Anderson, M.E., Determination of glutathione and glutathione disulfide in biological samples (1985) Method. Enzymol., 113, pp. 548-553
  • Arts, M.T., Ackman, R.G., Holub, B.J., Essential fatty acids in aquatic ecosystems: A crucial link between diet and human health and evolution (2001) Canadian Journal of Fisheries and Aquatic Sciences, 58, pp. 122-137
  • Ayala, V., Naudí, A., Sanz, A., Caro, P., Portero-Otin, M., Barja, G., Pamplona, R., Dietary protein restriction decreases oxidative protein damage, peroxidizability index, and mitochondrial complex I content in rat liver (2007) J. Gerontol. Ser. A: Biol. Sci. Med. Sci., 62, pp. 352-360
  • Bell, C.R., Dickie, G.A., Harvey, W.L.G., Chan, J.W.Y.F., Endophytic bacteria in grapevine (1995) Can. J. Microbiol., 41, pp. 46-53
  • Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification (1959) Can. J. Biochem. Physiol., 37, pp. 911-917
  • Bluhm, B.A., Brey, T., Klages, M., The autofluorescent age pigment lipofuscin: key to age, growth and productivity of the Antarctic amphipod Waldeckia obesa (Chevreux, 1905) (2001) J. Exp. Mar. Biol. Ecol., 258, pp. 215-235
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254
  • Carreira, R.S., Araújo, M.P., Costa, T.L.F., Spörl, G., Knoppers, B.A., Lipids in the sedimentary record as markers of the sources and deposition of organic matter in a tropical Brazilian estuarine-lagoon system (2011) Mar. Chem., 127, pp. 1-11
  • Cheung, S.G., Wai, H.Y., Shin, P.K.S., Fatty acid profiles of benthic environment associated with artificial reefs in subtropical Hong Kong (2010) Mar. Pollut. Bullet., 60, pp. 303-308
  • Christie, W.W., A simple procedure for rapid transmethylation of glycerolipids and cholesteryl esters (1982) J. Lipid Res., 23, pp. 1072-1075
  • Chu, F.-L.E., Greaves, J., Metabolism of palmitic, linoleic, and linolenic acids in adult oysters, Crassostrea virginica (1991) Mar. Biol., 110, pp. 229-236
  • Costa, T.L.F., Araujo, M.P., Knoppers, B.A., Renato, S.C., Sources and distribution of particulate organic matter of a tropical estuarine-lagoon system from NE Brazil as indicated by lipid biomarkers (2011) Aquat. Geochem., 17, pp. 1-19
  • De Moreno, J.E.A., Moreno, V.J., Brenner, R.R., Lipid metabolism of the yellow clam, Mesodesma mactroides: 2-polyunsaturated fatty acid metabolism (1976) Lipids, 11, pp. 561-566
  • De Moreno, J.E.A., Moreno, V.J., Brenner, R.R., Lipid metabolism of the yellow clam, Mesodesma mactroide: 3-saturated fatty acids and acetate metabolism (1977) Lipids, 12, pp. 804-808
  • Desvilettes, C., Bourdier, G., Breton, J.C., Combrouze, P., Fatty acids as organic markers for the study of trophic relationships in littoral cladoceran communities of a pond (1994) J. Plankton Res., 16, pp. 643-659
  • Di Salvatore, P., Calcagno, J.A., Ortíz, N., Ríos de Molina, M., Sabatini, S.E., Effect of seasonality on oxidative stress responses and metal accumulation in soft tissues of Aulacomya atra, a mussel from the South Atlantic Patagonian coast (2013) Mar. Environ. Res., 92, pp. 244-252
  • Diaz, M., Pedrozo, F., Temporetti, P., Phytoplankton of two Araucarian lakes of differing trophic status (Argentina) (1998) Hydrobiologia, 369 (370), pp. 45-57
  • Edelberg, C., (2004), Calidad del curso de agua superficial de la cuenca del arroyo Pocahullo. Tesis de Licenciatura. Universidad CAECE; Fraga, C.G., Leibovitz, B.E., Tappel, A.L., Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices. Characterization and comparison with homogenates and microsomes (1988) Free Rad. Biol. Med., 4, pp. 155-161
  • Gladyshev, M.I., Anishchenko, O.V., Sushchik, N.N., Kalacheva, G.S., Gribovskaya, I.V., Ageev, A.V., Influence of anthropogenic pollution on content of essential polyunsaturated fatty acids in links of food chain of river ecosystem (2012) Contemp. Probl. Ecol., 5, pp. 376-385
  • Gomes, A.D., Correia, E.T.G., Moreira, R.G., Fatty acids as trophic biomarkers in vitellogenic females in an impounded tropical river (2010) Fish Physiol. Biochem., 36, pp. 699-718
  • Habig, W.H., Pabst, M.J., Jakoby, W.B., Glutathione S-transferases: the first enzymatic step in mercapturic acid formation (1974) J. Biol. Chem., 249, pp. 7130-7139
  • Heras, H., Garín, C.F., Pollero, R.J., Biochemical composition and energy sources during embryo development and in early juveniles of the snail Pomacea canaliculata (Mollusca: Gastropoda) (1998) J. Exp. Zool., 280, pp. 375-383
  • Heras, H., Gonzalez-Baró, M.R., Pollero, R.J., Lipid and fatty acid composition and energy partitioning during embryo development in the shrimp Macrobrachium borellii (2000) Lipids, 35, pp. 645-651
  • Holman, R.T., Autoxidation of fats and related substances (1954) Prog. Chem. Fats Lipids, 2, pp. 51-98
  • Honkoop, P.J.C., Beukema, J.J., Loss of body mass in winter in three intertidal bivalve species: an experimental and observational study of the interacting effects between water temperature, feeding time and feeding behaviour (1997) J. Exp. Mar. Biol. Ecol., 212, pp. 277-297
  • Kainz, M., Arts, M.T., Mazumder, A., Essential versus potentially toxic dietary substances: a seasonal comparison of essential fatty acids and methyl mercury concentrations in the planktonic food web (2008) Environ. Pollut., 155, pp. 262-270
  • Kiffney, P.M., Richardson, J.S., Organic matter inputs into headwater streams of southwestern British Columbia as a function of riparian reserves and time since harvesting (2010) For. Ecol. Manage., 260, pp. 1931-1942
  • Koussoroplis, A.-M., Bec, A., Perga, M.-E., Koutrakis, E., Bourdier, G., Desvilettes, C., Fatty acid transfer in the food web of a coastal Mediterranean lagoon: evidence for high arachidonic acid retention in fish (2011) Estuar. Coast. Shelf Sci., 91, pp. 450-461
  • Lara, G., Contreras, A., Encina, E., La almeja de agua dulce Diplodon chilensis (Bivalvia:Hyriidae) potencial biofiltro para disminuir los niveles de coliformes en pozos (2002) Experimento de Laboratorio. Gayana, 66, pp. 113-118
  • Lau, D.C.P., Leung, K.M.Y., Dudgeon, D., Evidence of rapid shifts in the trophic base of lotic predators using experimental dietary manipulations and assimilation-based analyses (2009) Oecologia, 159, pp. 767-776
  • Lavarías, S., Pollero, R.J., Heras, H., Activation of lipid catabolism by the water-soluble fraction of petroleum in the crustacean Macrobrachium borellii (2006) Aquat. Toxicol., 77, pp. 190-196
  • Lavarías, S., García, F., Pollero, R.J., Heras, H., Effect of the water-soluble fraction of petroleum on microsomal lipid metabolism of Macrobrachium borellii (Arthropoda: Crustacea) (2007) Aquat. Toxicol., 82, pp. 265-271
  • Leveille, J.C., Amblard, C., Bourdier, G., Fatty acids as specific algal markers in a natural lacustrian phytoplankton (1997) J. Plankton Res., 19, pp. 469-490
  • Lomovasky, B.J., Morriconi, E., Brey, T., Calvo, J., Individual age and connective tissue lipofuscin in the hard clam Eurhomalea exalbida (2002) J. Exp. Mar. Biol. Ecol., 276, pp. 83-94
  • Luca-Abbott, S.D., Biomarkers of sublethal stress in the soft-sediment bivalve Austrovenus stutchburyi exposed in-situ to contaminated sediment in an urban New Zealand harbour (2001) Mar. Pollut. Bullet., 42, pp. 817-825
  • Masuda, R., Tsukamoto, K., School formation and concurrent developmental changes in carangid fish with reference to dietary conditions (1999) Environ. Biol. Fish., 56, pp. 243-252
  • Meziane, T., Tsuchiya, M., Organic matter in a subtropical mangrove-estuary subjected to wastewater discharge: origin and utilisation by two macrozoobenthic species (2002) J. Sea Res., 47, pp. 1-11
  • Mitchell, T.W., Buffenstein, R., Hulbert, A.J., Membrane phospholipid composition may contribute to exceptional longevity of the naked mole-rat (Heterocephalus glaber): A comparative study using shotgun lipidomics (2007) Exp. Gerontol., 42, pp. 1053-1062
  • Montero, D., Robaina, L.E., Socorro, J., Vergara, J.M., Tort, L., Izquierdo, M.S., Alteration of liver and muscle fatty acid composition in gilthead seabream (Sparus aurata) juveniles held at high stocking density and fed an essential fatty acid deficient diet (2001) Fish Physiol. Biochem., 24, pp. 63-72
  • Montero, D., Kalinowski, T., Obach, A., Robaina, L., Tort, L., Caballero, M.J., Izquierdo, M.S., Vegetable lipid sources for gilthead seabream (Sparus aurata): effects on fish health (2003) Aquaculture, 225, pp. 353-370
  • Montero, D., Socorro, J., Tort, L., Caballero, M.J., Robaina, L.E., Vergara, J.M., Izquierdo, M.S., Glomerulonephritis and immunosuppression associated with dietary essential fatty acid deficiency in gilthead sea bream, Sparus aurata L., juveniles (2004) J. Fish Dis., 27, pp. 297-306
  • Moore, M.N., Bubel, A., Lowe, D.M., Cytology and cytochemistry of the pericardial gland cells of Mytilus edulis and their lysosomal responses to injected horseradish peroxidase and anthracene (1980) J. Mar. Biol. Assoc. UK, 60, pp. 135-149
  • Munro, D., Blier, P.U., The extreme longevity of Arctica islandica is associated with increased peroxidation resistance in mitochondrial membranes (2012) Aging Cell, 11, pp. 845-855
  • Napolitano, G.E., Fatty acids as trophic and chemical markers in freshwater ecosystems (1999) Lipids in freswater ecosystems, pp. 21-44. , Springer, New York, M.T. Arts, B.C. Wainman (Eds.)
  • Napolitano, G.E., Heras, H., Stewart, A.J., Fatty acid composition of freshwater phytoplankton during a red tide event (1995) Biochem. Syst. Ecol., 23, pp. 65-69
  • Napolitano, G.E., Pollero, R.J., Gayoso, A.M., Macdonald, B.A., Thompson, R.J., Fatty acids as trophic markers of phytoplankton blooms in the Bahía Blanca estuary (Buenos Aires, Argentina) and in Trinity Bay (Newfoundland, Canada) (1997) Biochem. Syst. Ecol., 25, pp. 739-755
  • Navarrete, A., Peacock, A., Macnaughton, S.J., Urmeneta, J., Mas-Castella, J., White, D.C., Guerrero, R., Physiological status and community composition of microbial mats of the Ebro Delta, Spain, by signature lipid biomarkers (2000) Microbial Ecology, 39, pp. 92-99
  • Pamplona, R., Membrane phospholipids, lipoxidative damage and molecular integrity: a causal role in aging and longevity (2008) BBA-Bioenergetics, 1777, pp. 1249-1262
  • Pamplona, R., Portero-Otín, M., Requena, J., Gredilla, R., Barja, G., Oxidative glycoxidative and lipoxidative damage to rat heart mitochondrial proteins is lower after 4months of caloric restriction than in age-matched controls (2002) Mech. Ageing Develop., 123, pp. 1437-1446
  • Parada, E., Peredo, S., Cárdenas, S., Valdebenito, I., Peredo, M., Diplodon chilensis Gray, 1828 (Bivalvia:Hyriidae) un potencial depurador de aguas residuales de piscicultura de salmonidos de aguas continentales: Un estudio a escala de laboratorio (2008) Gayana, 72, pp. 68-78
  • Parrish, C., Abrajano, T., Budge, S., Helleur, R., Hudson, E., Pulchan, K., (2000) Lipid and Phenolic Biomarkers in Marine Ecosystems: Analysis and Applications, , Springer, Berlin
  • Pasquevich, M.Y., Dreon, M.S., Lavarías, S., Heras, H., Triacylglycerol catabolism in the prawn Macrobrachium borellii (Crustacea: Palaemoniade) (2011) Comp. Biochem. Physiol. Part B: Biochem. Mol. Biol., 160, pp. 201-207
  • Penha-Lopes, G., Torres, P., Narciso, L., Cannicci, S., Paula, J., Comparison of fecundity, embryo loss and fatty acid composition of mangrove crab species in sewage contaminated and pristine mangrove habitats in Mozambique (2009) J. Exp. Mar. Biol. Ecol., 381, pp. 25-32
  • Perga, M.E., Bec, A., Anneville, O., Origins of carbon sustaining the growth of whitefish Coregonus lavaretus early larval stages in Lake Annecy: insights from fatty-acid biomarkers (2009) J. Fish Biol., 74, pp. 2-17
  • Perrat, E., Couzinet-Mossion, A., Fossi Tankoua, O., Amiard-Triquet, C., Wielgosz-Collin, G., Variation of content of lipid classes, sterols and fatty acids in gonads and digestive glands of Scrobicularia plana in relation to environment pollution levels (2013) Ecotoxicol. Environ. Safe., 90, pp. 112-120
  • Pollero, R.J., Brenner, R.R., Effect of the environment and fasting on the lipid and fatty acid composition of Diplodon patogonicus (1981) Lipids, 16, pp. 685-690
  • Pollero, R.J., Brenner, R.R., Gros, E.G., Seasonal changes in lipid and fatty acid composition of the freshwater mollusk, Diplodon patagonicus (1981) Lipids, 16, pp. 109-113
  • Pond, D.W., Tarling, G.A., Phase transitions of wax esters adjust buoyancy in diapausing calanoides acutus (2011) Limnol. Oceanogr., 56, pp. 1310-1318
  • Portero-Otin, M., Bellmunt, M.J., Ruiz, M.C., Correlation of fatty acid unsaturation of the major liver mitochondrial phospholipid classes in mammals to their potential life span (2001) Lipids, 36, pp. 491-498
  • Reznick, A.Z., Packer, L., Oxidative damage to proteins: Spectrophotometric methods for carbonyl assay (1994) Method. Enzymol., 38, pp. 357-363
  • Ribeiro, S., Sousa, J.P., Nogueira, A.J.A., Soares, A.M.V.M., Effect of endosulfan and parathion on energy reserves and physiological parameters of the terrestrial isopod Porcellio dilatatus (2001) Ecotoxicol. Environ. Safe., 49, pp. 131-138
  • Rocchetta, I., Mazzuca, M., Conforti, V., Ruiz, L., Balzaretti, V., de Molina, M., Effect of chromium on the fatty acid composition of two strains of Euglena gracilis (2006) Environ. Pollut., 141, pp. 353-358
  • Rocchetta, I., Mazzuca, M., Conforti, V., Balzaretti, V., Molina, M., Chromium induced stress conditions in heterotrophic and auxotrophic strains of Euglena gracilis (2012) Ecotoxicol. Environ. Safe., 84, pp. 147-154
  • Rocchetta, I., Lomovasky, B.J., Yusseppone, M.S., Sabatini, S.E., Bieczynski, F., Ríos de Molina, M.C., Luquet, C.M., Growth, abundance, morphometric and metabolic parameters of three populations of Diplodon chilensis subject to different levels of natural and anthropogenic organic matter input in a glaciar lake of North Patagonia (2014) Limnol. - Ecol. Manage. Inland Waters, 44, pp. 72-80
  • Romero, D.M., Ríos de Molina, M.C., Juárez, A.B., Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri (2011) Ecotoxicol. Environ. Safe., 74, pp. 741-747
  • Sabatini, S.E., Juárez, A.B., Eppis, M.R., Bianchi, L., Luquet, C.M., Ríos de Molina, M., Oxidative stress and antioxidant defenses in two green microalgae exposed to copper (2009) Ecotoxicol. Environ. Safe., 72, pp. 1200-1206
  • Sabatini, S.E., Rocchetta, I., Luquet, C.M., Guido, M.I., de Molina, M., Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis (2011) Limnol. - Ecol. Manage. Inland Waters, 41, pp. 356-362
  • Sakdullah, A., Tsuchiya, M., The origin of particulate organic matter and the diet of tilapia from an estuarine ecosystem subjected to domestic wastewater discharge: fatty acid analysis approach (2009) Aquatic Ecology, 43, pp. 577-589
  • Sarà, G., Reid, G.K., Rinaldi, A., Palmeri, V., Troell, M., Kooijman, S.A.L.M., Growth and reproductive simulation of candidate shellfish species at fish cages in the Southern Mediterranean: Dynamic Energy Budget (DEB) modelling for integrated multi-trophic aquaculture (2012) Aquaculture, pp. 259-266
  • Sokal, R.R., Rohlf, F.J., (1984), Introducción a la bioestadística, España; Sprecher, H., Biochim. Biophys. Acta 1486, 219-231, 2000. Metabolism of highly unsaturated n-3 and n-6 fatty acids (2000) Biochim. Biophys. Acta, 1486, pp. 219-231
  • Strahl, J., Philipp, E., Brey, T., Broeg, K., Abele, D., Physiological aging in the Icelandic population of the ocean quahog Arctica islandica (2007) Aquat. Biol., 1, pp. 77-83
  • Sushchik, N.N., Gladyshev, M.I., Moskvichova, A.V., Makhutova, O.N., Kalachova, G.S., Comparison of fatty acid composition in major lipid classes of the dominant benthic invertebrates of the Yenisei river (2003) Comp. Biochem. Physiol. Part B: Biochem. Mol. Biol., 134, pp. 111-122
  • Temporetti, P., Baffico, G., Diaz, M., Pedrozo, F., (2009), Estado trófico del lago Lacar y del arroyo Pocahullo. Influencia de la descarga de líquidos cloacales en la cuenca oriental del lago Technical report: Un puente entre la Universidad y la Sociedad. Secretaría de Políticas Universitarias, Ministerio de Educación Ciencia y Tecnología. Universidad Nacional del Comahue; Valdovinos, C., Pedreros, P., Geographic variations in shell growth rates of the mussel Diplodon chilensis from temperate lakes of Chile: implications for biodiversity conservation (2007) Limnol. - Ecol. Manage. Inland Waters, 37, pp. 63-75
  • Van Handel, E., Estimation de glycogen in small amount soft tissue (1965) Anal. Biochem., 11, pp. 256-265
  • Volk, C., Kiffney, P., Comparison of fatty acids and elemental nutrients in periphyton, invertebrates, and cutthroat trout (Oncorhynchus clarki) in conifer and alder streams of western Washington state (2012) Aquatic Ecology, 46, pp. 85-99
  • Volkman, J.K., Barrett, S.M., Blackburn, S.I., Mansour, M.P., Sikes, E.L., Gelin, F., Microalgal biomarkers: a review of recent research developments (1998) Organic Geochemistry, 29, pp. 1163-1179
  • Wacker, A., Becher, P., Von Elert, E., Food quality effects of unsaturated fatty acids on larvae of the zebra mussel Dreissena polymorpha (2002) Limnol. Oceanogr., 47, pp. 1242-1248
  • Whyte, J.N.C., Englar, J.R., Carswell, B.L., Biochemical composition and energy reserves in Crassostrea gigas exposed to different levels of nutrition (1990) Aquaculture, 90, pp. 157-172
  • Zhukova, N.V., The pathway of the biosynthesis of non-methylene-interrupted dienoic fatty acids in molluscs (1991) Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 100, pp. 801-804
  • Zhukova, N.V., Svetashev, V.I., Non-methylene-interrupted dienoic fatty acids in molluscs from the sea of Japan (1986) Comparative Biochemistry and Physiology -Part B: Biochemistry and Molecular Biology, 83, pp. 643-646

Citas:

---------- APA ----------
Rocchetta, I., Pasquevich, M.Y., Heras, H., Ríos de Molina, M.D.C. & Luquet, C.M. (2014) . Effects of sewage discharges on lipid and fatty acid composition of the Patagonian bivalve Diplodon chilensis. Marine Pollution Bulletin, 79(1-2), 211-219.
http://dx.doi.org/10.1016/j.marpolbul.2013.12.011
---------- CHICAGO ----------
Rocchetta, I., Pasquevich, M.Y., Heras, H., Ríos de Molina, M.D.C., Luquet, C.M. "Effects of sewage discharges on lipid and fatty acid composition of the Patagonian bivalve Diplodon chilensis" . Marine Pollution Bulletin 79, no. 1-2 (2014) : 211-219.
http://dx.doi.org/10.1016/j.marpolbul.2013.12.011
---------- MLA ----------
Rocchetta, I., Pasquevich, M.Y., Heras, H., Ríos de Molina, M.D.C., Luquet, C.M. "Effects of sewage discharges on lipid and fatty acid composition of the Patagonian bivalve Diplodon chilensis" . Marine Pollution Bulletin, vol. 79, no. 1-2, 2014, pp. 211-219.
http://dx.doi.org/10.1016/j.marpolbul.2013.12.011
---------- VANCOUVER ----------
Rocchetta, I., Pasquevich, M.Y., Heras, H., Ríos de Molina, M.D.C., Luquet, C.M. Effects of sewage discharges on lipid and fatty acid composition of the Patagonian bivalve Diplodon chilensis. Mar. Pollut. Bull. 2014;79(1-2):211-219.
http://dx.doi.org/10.1016/j.marpolbul.2013.12.011