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Abstract:

The early ontogenetic stages of the sub-Antarctic king crab Lithodes santolla were analyzed for the presence and activities of a set of important digestive enzymes. The eggs and non-feeding larvae (zoea I-III, megalopa) showed high activities of esterases, phosphatases, and exopeptidases indicating the enzymatic ability to utilize endogeneous yolk reserves. SDS-PAGE showed a continuous decrease of proteins or proteids in the range of 59-81 kDa during ontogenetic development from the eggs through the zoeal stages to the first juvenile crab stage, CI. This reduction reflects the degradation of storage compounds during lecithotrophic larval development. Activities of the endopeptidases, trypsin and chymotrypsin, were low in eggs and larvae but increased significantly in the first juvenile crab stage. These enzymes typically facilitate the first steps of proteolysis in the extra-cellular spaces of the midgut gland and in the stomach. Their scarcity indicates that the larvae of L. santolla are physiologically not prepared to digest external food. This ability seems to appear first in the CI stage. Extracts of juvenile midgut glands and the gastric fluids of adults showed high activities of a variety of digestive enzymes including phosphatases, carbohydrases, as well as endo- and exopeptidases. High activities of digestive enzymes in adults may compensate for scarce food supply and rate-limiting low temperatures in the predominantly sub-Antarctic habitats of L. santolla. © Springer-Verlag 2006.

Registro:

Documento: Artículo
Título:Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla
Autor:Saborowski, R.; Thatje, S.; Calcagno, J.A.; Lovrich, G.A.; Anger, K.
Filiación:Biologische Anstalt Helgoland, Foundation Alfred Wegener Institute for Polar and Marine Research, 27498, Helgoland, Germany
Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27515, Bremerhaven, Germany
National Oceanography Centre, Southampton, University of Southampton, European Way, SO14 3ZH, Southampton, United Kingdom
Facultad de Sciencias Exactas Y Naturales, Universidad Buenos Aires, Cdad. Universitaria, Intendante Güiraldes 2160, C1428EHA, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas Y Técnicas, Centro Ausral de Investigaciones Científicas, CC 92, V9410BFD, Ushuaia, Tierra del Fuego, Argentina
Palabras clave:crab; enzyme activity; food availability; juvenile; larval development; low temperature; ontogeny; Antarctica; Chaceon affinis; Decapoda (Crustacea); Limulus; Lithodes santolla; Proteidae
Año:2006
Volumen:149
Número:4
Página de inicio:865
Página de fin:873
DOI: http://dx.doi.org/10.1007/s00227-005-0240-x
Título revista:Marine Biology
Título revista abreviado:Mar. Biol.
ISSN:00253162
CODEN:MBIOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00253162_v149_n4_p865_Saborowski

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Citas:

---------- APA ----------
Saborowski, R., Thatje, S., Calcagno, J.A., Lovrich, G.A. & Anger, K. (2006) . Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla. Marine Biology, 149(4), 865-873.
http://dx.doi.org/10.1007/s00227-005-0240-x
---------- CHICAGO ----------
Saborowski, R., Thatje, S., Calcagno, J.A., Lovrich, G.A., Anger, K. "Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla" . Marine Biology 149, no. 4 (2006) : 865-873.
http://dx.doi.org/10.1007/s00227-005-0240-x
---------- MLA ----------
Saborowski, R., Thatje, S., Calcagno, J.A., Lovrich, G.A., Anger, K. "Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla" . Marine Biology, vol. 149, no. 4, 2006, pp. 865-873.
http://dx.doi.org/10.1007/s00227-005-0240-x
---------- VANCOUVER ----------
Saborowski, R., Thatje, S., Calcagno, J.A., Lovrich, G.A., Anger, K. Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla. Mar. Biol. 2006;149(4):865-873.
http://dx.doi.org/10.1007/s00227-005-0240-x