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Abstract:

We studied Na+/K+-ATPase activity and ultrastructure in gills of the hyper-hypo-regulating crab Chasmagnathus granulatus Dana, 1851 acclimated to different salinities: 10, 30 and 45‰, known to be hypo-, iso-, and hyper-osmotic to the hemolymph, respectively. After centrifugation of homogenates at 11,000 g, Na+/ K+-ATPase activity was almost entirely found in the pellets from the posterior (6-8) and anterior (3-5) gills, whereas very little was detected in the supernatant liquid. Specific activity of gill 6 was 41.3, 30.2, and 28.2 μmol Pi h-1 mg prot-1 for crabs acclimated to 10, 30, and 45‰, respectively, the result for 10‰ being significantly higher than those at 30 and 45‰. Although the concentration of sodium at which the reaction rate is half-maximal (KM) was similar in the three acclimation salinities, only the enzyme from crabs acclimated to 10‰ was inhibited by high sodium concentration. Specific activity of gill 5 increased with the increment in external salinity (10.1, 15, and 18.1 μmol Pi h-1 mg prot -1 for 10, 30, and 45‰, respectively), the only significant difference being that between the extreme salinities. The epithelium thickness of the dorsal portion of gill 6 showed a variation among salinities: 21.7, 15.8 and 17.2 μm for 10, 30 and 45‰, respectively. There were significant differences in epithelium thickness between the 10‰ and the other salinities. In all three salinities, the ultrastructure of gill 6 epithelium showed a high density of mitochondria, estimated by their volume fraction (Vvm = 0.307-0.355). These mitochondria were packed between extensive basolateral membrane interdigitations in ionocytes and pillar cells. Gill 5 showed three cell types: pillars which possess mitochondria packed between membrane folds only in their interdigitations with neighbouring cells; type-I cells 8.0 μm thick with low density of mitochondria (Vvm = 0.088), and type-II cells, 9.9 μm thick and rich in mitochondria (Vv m = 0.423), but lacking basolateral interdigitations. Vvm of type-I cells of gill 5 was significantly lower than those of type-II cells of the same gill and the ionocytes of gill 6. No significant difference in Vvm was detected between the latter cell types.

Registro:

Documento: Artículo
Título:Na+/K+-ATPase activity and gill ultrastructure in the hyper-hypo-regulating crab Chasmagnathus granulatus acclimated to dilute, normal, and concentrated seawater
Autor:Genovese, G.; Luchetti, C.G.; Luquet, C.M.
Filiación:Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Rivadavia 1917 (C1033AAJ), CONICET, Buenos Aires, Argentina
Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria (C1428EHA), Buenos Aires, Argentina
Palabras clave:crab; ultrastructure; Chasmagnathus granulata; Decapoda (Crustacea)
Año:2004
Volumen:144
Número:1
Página de inicio:111
Página de fin:118
DOI: http://dx.doi.org/10.1007/s00227-003-1169-6
Título revista:Marine Biology
Título revista abreviado:Mar. Biol.
ISSN:00253162
CODEN:MBIOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00253162_v144_n1_p111_Genovese

Referencias:

  • Barra, J.A., Péqueux, A., Humbert, W., A morphological study on gills of a crabs acclimated to fresh water (1983) Tissue Cell, 15, pp. 583-596
  • Boschi, E.E., Los crustáceos decápodos brachyura del litoral bonaerense (1964) Bol Inst Biol Mar Mar del Plata, 164, pp. 1-34
  • Brown, D., Breton, S., Mitochondria-rich, proton-secreting epithelial cells (1996) J Exp Biol, 199, pp. 2345-2358
  • Castilho, P., Martins, I.A., Bianchini, A., Gill Na+, K+-ATPase and osmoregulation in the estuarine crab, Chasmagnathus granulata Dana, 1851 (Decapoda, Grapsidae) (2001) J Exp Mar Biol Ecol, 256, pp. 215-227
  • Charmantier, G., Gimenez, L., Charmantier-Daures, M., Anger, K., Ontogeny of osmoregulation, physiological plasticity and larval export strategy in the grapsid crab Chasmagnathus granulata (Crustacea, Decapoda) (2002) Mar Ecol Prog Ser, 229, pp. 185-194
  • Compère, P., Wanson, S., Péqueux, A., Gilles, R., Goffinet, G., Ultrastructural changes in the gill epithelium of the green crab Carcinus maenas in relation to the external salinity (1989) Tissue Cell, 21, pp. 299-318
  • Copeland, D.E., Fine structure of salt and water uptake in the land-crab, Gecarcinus lateralis (1968) Am Zool, 8, pp. 417-432
  • D'Orazio, S.E., Holliday, C.W., Gill Na,K-ATPase and osmoregulation in the sand fiddler crab, Uca pugilator (1985) Physiol Zool, 58, pp. 364-373
  • Drach, P., Tchernigovtzeff, C., Sur la méthode de détermination des stades d'intermue et son application générale aux crustacés (1967) Vie Milieu, 18, pp. 597-607
  • Ernst, S.A., Dodson, W.C., Karnaki, K.J., Structural diversity of occluding junctions in the low-resistance chloride-secreting opercular epithelium of seawater-adapted killifish (Fundulus heteroclitus) (1980) J Cell Biol, 87, pp. 488-497
  • Genovese, G., Luquet, C.M., Paz, D.A., Rosa, G.A., Pellerano, G.N., The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hypo-regulation conditions are not caused by proliferation of specialised cells (2000) J Anat, 197, pp. 239-246
  • Gilles, R., Péqueux, A., Physiological and ultrastructural studies of NaCl transport in crustacean gills (1986) Boll Zool, 53, pp. 173-182
  • Harris, R.R., Bayliss, D., Gill (Na+ + K+)-ATPases in decapod crustaceans: Distribution and characteristics in relation to Na+ regulation (1988) Comp Biochem Physiol, 90 A, pp. 303-308
  • Harris, R.R., Santos, M.C.F., Sodium uptake and transport (Na+ + K+)ATPase changes following Na+ depletion and low salinity acclimation in the mangrove crab Ucides cordatus (L.) (1993) Comp Biochem Physiol, 105 A, pp. 35-42
  • Henry, R.P., Garrelts, E.E., McCarty, M.M., Towle, D.W., Differential induction of branchial carbonic anhydrase and Na +/K+ ATPase activity in the euryhaline crab Carcinus maenas, in response to low salinity exposure (2002) J Exp Zool, 292, pp. 595-603
  • Holliday, C.W., Salinity-induced changes in gill Na,K-ATPase activity in the mud fiddler, Uca pugnax (1985) J Exp Biol, 233, pp. 199-208
  • Lowry, O.H., Rosenbrough, N.J., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275
  • Lowy, R.J., Dawson, D.C., Ernst, S.A., Mechanism of ion transport by avian salt gland primary cell cultures (1989) Am J Physiol, 256, pp. R1184-R1191
  • Lucu, C., Flik, G., Na+-K+-ATPaSe and Na+/Ca+ exchange activities in gills of hyperregulating Carcinus maenas (1999) Am J Physiol, 276, pp. R490-R499
  • Luquet, C.M., Pellerano, G., De Carlo, J.M., Gill morphology and terrestrial adaptation in the estuarine crab Uca uruguayensis (Nobili, 1901) (Decapoda, Brachyura) (1995) Crustaceana, 68, pp. 882-892
  • Luquet, C.M., Pellerano, G., Rosa, G., Salinity-induced changes in the fine structure of the gills of the semiterrestrial estuarine crab Uca uruguavensis (Nobili, 1901) (Decapoda, Ocypodidae) (1997) Tissue Cell, 29, pp. 495-501
  • Luquet, C.M., Rosa, G.A., Ferrari, C.C., Genovese, G., Pellerano, G.N., Gill morphology of the intertidal estuarine crab Chasmagnathus granulata Dana, 1851 (Decapoda, Grapsidae) in relation to habitat and respiratory habits (2000) Crustaceana, 73, pp. 53-67
  • Luquet, C.M., Genovese, G., Rosa, G.A., Pellerano, G.N., Ultrastructural changes in the gill epithelium of the crab Chasmagnathus granulatus (Decapoda, Grapsidae) in dilute and concentrated seawater (2002) Mar Biol, 141, pp. 753-760
  • Luquet, C.M., Postel, U., Halperin, J., Urcola, M.R., Marques, R., Siebers, D., Transepithelial potential differences and sodium flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-saline media (2002) J Exp Biol, 205, pp. 71-77
  • McNamara, J.C., Torres, A.H., Ultracytochemical location of Na+/K+-ATPase activity and effect of high salinity acclimation in gill and renal epithelia of the freshwater shrimp Macrobrachium olfersii (Crustacea Decapoda) (1999) J Exp Zool, 284, pp. 617-628
  • Mougabure Cueto, G.A., (1998) Interacción Entre la Regulación Iónica y El Equilibrio Ácido-base en El Cangrejo Chasmagnathus Granulata Dana 1851 (Decapoda, Grapsidae), , Graduate thesis, Universidad de Buenos Aires, Buenos Aires
  • Neufeld, G.J., Holliday, C.W., Pritchard, J.B., Salinity adaption of gill Na,K-ATPase in the blue crab, Callinectes sapidus (1980) J Exp Zool, 211, pp. 215-224
  • Péqueux, A., Osmotic regulation in crustaceans (1995) J Crustac Biol, 15, pp. 1-60
  • Péqueux, A., Marchal, A., Wanson, S., Gilles, R., Kinetic characteristics and specific activity of gill (Na+ + K+)-ATPase in the euryhaline Chinese crab Eriocheir sinensis during salinity acclimation (1984) Mar Biol Lett, 5, pp. 35-45
  • Piller, S.C., Henry, R.P., Doeller, J.E., Kraus, D.W., A comparison of the gill physiology of two euryhaline crab species, Callinectes sapidus and Callinectes similis: Energy production, transport-related enzymes and osmoregulation as a function of acclimation salinity (1995) J Exp Biol, 198, pp. 349-358
  • Quinn, D.J., Lane, C.E., Ionic regulation and Na+-K+-stimulated ATPase activity in the land crab, Cardisoma guanhumi (1966) Comp Biochem Physiol, 19, pp. 533-543
  • Schubart, C.D., Cuesta, J.A., Felder, D.L., Glyptograpsidae, a new brachyuran family from Central America: Larval and adult morphology, and a molecular phylogeny of the Grapsoidea (2002) J Crustac Biol, 22, pp. 28-44
  • Siebers, D., Leweck, K., Markus, H., Winkler, A., Sodium regulation in the shore crab Carcinus maenas as related to ambient salinity (1982) Mar Biol, 69, pp. 37-43
  • Siebers, D., Winkler, A., Lucu, C., Thedens, G., Weichart, D., NaK-ATPase generates an active transport potential in the gills of the hyperregulating shore crab Carcinus maenas (1985) Mar Biol, 87, pp. 185-192
  • Siebers, D., Lucu, C., Winkler, A., Dalla Venezia, L., Wille, H., Active uptake of sodium in the gills of the hyperregulating shore crab Carcinus maenas (1986) Helgol Wiss Meeresunters, 40, pp. 151-160
  • Silva, P., Stoff, J., Field, M., Fine, L., Mechanism of active chloride secretion by shark rectal gland: Role of Na-K-ATPase in chloride transport (1977) Am J Physiol, 233, pp. F298
  • Skou, J.C., Enzymatic basis for active transport of Na+ and K+ across the cell membrane (1965) Physiol Rev, 45, pp. 596-617
  • Taylor, H.H., Taylor, E.W., Gills and lungs: The exchange of gases and ions (1992) Microscopic Anatomy of Invertebrates, 10, pp. 203-293. , Harrison FW, Humes AG (eds), Wiley-Liss, New York
  • Towle, D.W., Membrane-bound ATPases in arthropod ion-transporting tissues (1984) Am Zool, 24, pp. 177-185
  • Towle, D.W., Kays, W.T., Basolateral localization of Na+ + K+-ATPase in gill epithelium of two osmoregulating crabs, Callinectes sapidus and Carcinus maenas (1986) J Exp Zool, 239, pp. 311-318
  • Weibel, E.R., (1989) Stereological Methods, pp. 1-415. , Academic Press, San Diego
  • Weihrauch, D., Becker, W., Postel, U., Riestenpatt, S., Siebers, D., Active excretion of ammonia across the gills of the shore crab Carcinus maenas and its relation to osmoregulatory ion uptake (1998) J Comp Physiol, 168, pp. 364-376
  • Weihrauch, D., Becker, W., Postel, U., Luck-Kopp, S., Siebers, D., Potential of active excretion of ammonia in three differently haline species of crabs (1999) J Comp Physiol, 169, pp. 25-37

Citas:

---------- APA ----------
Genovese, G., Luchetti, C.G. & Luquet, C.M. (2004) . Na+/K+-ATPase activity and gill ultrastructure in the hyper-hypo-regulating crab Chasmagnathus granulatus acclimated to dilute, normal, and concentrated seawater. Marine Biology, 144(1), 111-118.
http://dx.doi.org/10.1007/s00227-003-1169-6
---------- CHICAGO ----------
Genovese, G., Luchetti, C.G., Luquet, C.M. "Na+/K+-ATPase activity and gill ultrastructure in the hyper-hypo-regulating crab Chasmagnathus granulatus acclimated to dilute, normal, and concentrated seawater" . Marine Biology 144, no. 1 (2004) : 111-118.
http://dx.doi.org/10.1007/s00227-003-1169-6
---------- MLA ----------
Genovese, G., Luchetti, C.G., Luquet, C.M. "Na+/K+-ATPase activity and gill ultrastructure in the hyper-hypo-regulating crab Chasmagnathus granulatus acclimated to dilute, normal, and concentrated seawater" . Marine Biology, vol. 144, no. 1, 2004, pp. 111-118.
http://dx.doi.org/10.1007/s00227-003-1169-6
---------- VANCOUVER ----------
Genovese, G., Luchetti, C.G., Luquet, C.M. Na+/K+-ATPase activity and gill ultrastructure in the hyper-hypo-regulating crab Chasmagnathus granulatus acclimated to dilute, normal, and concentrated seawater. Mar. Biol. 2004;144(1):111-118.
http://dx.doi.org/10.1007/s00227-003-1169-6