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Abstract:

Sequence analysis of the internal transcribed spacer (ITS) of the 18S(ITS1)-5.8S-26S(ITS2) rDNA region was performed in order to analyse the phylogenetic relationships between 13 Patagonian species of the genus Berberis (Berberidaceae). The divergence values between the pairwise sequence in the studied Patagonian species were in the range 2.9-22.9%. The lengths of the ITS1 and ITS2 sequences were in the range 227-231 bp and 220-224 bp, respectively, and the 5.8S sequence was 159 bp throughout all species. B. microphylla sensu Landrum does not appear to be monophyletic based on current sampling. Indeed, we suggest that B. microphylla should be distinguished from B. buxifolia, B. parodii, and B. heterophylla. ITS sequences, together with data obtained from morphological, biochemical, amplified fragment length polymorphism, and cytological characterizations, support the existence of diploid and polyploid hybrid speciation in the genus. © 2007 The Linnean Society of London.

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Documento: Artículo
Título:Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences
Autor:Bottini, M.C.J.; De Bustos, A.; Sanso, A.M.; Jouve, N.; Poggio, L.
Filiación:Instituto de Ecología y Evolución, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes y Costanera Norte (C1428EHA), Buenos Aires, Argentina
CIGen-CONICET (B1836AML) Llavallol, Argentina
Department of Cell Biology and Genetics, University of Alcalá, Campus Universidad de Alcalá, Alcalá de Henares, Madrid, Spain
Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Paraje Arroyo Seco s/n, (B7000) Tandil, Argentina
Palabras clave:Argentina; Barberry; Calafate; Diploid and polyploid hybrid speciation; Michay; Molecular markers; DNA fingerprinting; genetic differentiation; hybridization; molecular analysis; phylogenetics; polymorphism; polyploidy; shrub; speciation (biology); Argentina; South America; Berberidaceae; Berberis; Berberis buxifolia; Berberis heterophylla; Berberis microphylla; Berberis parodii; Landrum
Año:2007
Volumen:153
Número:3
Página de inicio:321
Página de fin:328
DOI: http://dx.doi.org/10.1111/j.1095-8339.2007.00586.x
Título revista:Botanical Journal of the Linnean Society
Título revista abreviado:Bot. J. Linn. Soc.
ISSN:00244074
CODEN:BJLSA
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00244074_v153_n3_p321_Bottini.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00244074_v153_n3_p321_Bottini

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Citas:

---------- APA ----------
Bottini, M.C.J., De Bustos, A., Sanso, A.M., Jouve, N. & Poggio, L. (2007) . Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences. Botanical Journal of the Linnean Society, 153(3), 321-328.
http://dx.doi.org/10.1111/j.1095-8339.2007.00586.x
---------- CHICAGO ----------
Bottini, M.C.J., De Bustos, A., Sanso, A.M., Jouve, N., Poggio, L. "Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences" . Botanical Journal of the Linnean Society 153, no. 3 (2007) : 321-328.
http://dx.doi.org/10.1111/j.1095-8339.2007.00586.x
---------- MLA ----------
Bottini, M.C.J., De Bustos, A., Sanso, A.M., Jouve, N., Poggio, L. "Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences" . Botanical Journal of the Linnean Society, vol. 153, no. 3, 2007, pp. 321-328.
http://dx.doi.org/10.1111/j.1095-8339.2007.00586.x
---------- VANCOUVER ----------
Bottini, M.C.J., De Bustos, A., Sanso, A.M., Jouve, N., Poggio, L. Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences. Bot. J. Linn. Soc. 2007;153(3):321-328.
http://dx.doi.org/10.1111/j.1095-8339.2007.00586.x