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Abstract:

We investigated the taxonomic status of two sympatric morphospecies of squat lobsters from southern South America (Beagle Channel, Strait of Magellan, and Burdwood Bank), Munida gregaria and Munida subrugosa, by DNA sequence analysis of three mitochondrial (mt)DNA gene fragments [416 bp of 16S rDNA(165), 566 bp of cytochrome c oxidase subunit I(COI) and 418 bp of NADH dehydrogenase subunit 1 (ND1)]; and the nuclear rDNA internal transcribed spacer (ITS) 1 (883-952 bp). We obtained a total of 79 sequences from 32 individuals. The 16S sequences of all M. gregaria and M. subrugosa were invariant and identical, whereas COI and ND1 showed 12 and 15 variable sites, respectively. These polymorphisms were shared between morphospecies. Interspecific Tamura-Nei distances for COI and ND1 sequences were 0.0024 and 0.0032, respectively, and were not significantly different from intraspecific distances (Kruskal-Wallis tests: P = 0.58 and P = 0.69, for COI and ND1, respectively). Similar to the results obtained from the mtDNA sequences, no relationship was found between the ITS1 maximum parsimony tree topology and the morphologic classification of specimens in M. gregaria and M. subrugosa. We conclude that M. gregaria and M. subrugosa from southern South America may either represent a case of a dimorphic species, or a case of incomplete lineage sorting. The fact that these two morphospecies did not show fixed differences over a total of 1947 bp analysed reinforces the hypothesis of a single dimorphic species. © 2008 The Linnean Society of London.

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Documento: Artículo
Título:Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information
Autor:Pérez-Barros, P.; D'Amato, M.E.; Guzmán, N.V.; Lovrich, G.A.
Filiación:Centro Austral de Investigaciones Científicas, Consejo Nacional de Investigaciones Científicas Y Técnicas (CADIC - CONICET), B. Houssay 200, V9410CAB Ushuaia, Tierra del Fuego, Argentina
Stellenbosch University, Genetics Department, Aquaculture Division, Private Bag X1, 7602 Matieland, South Africa
Universidad de Buenos Aires, Facultad de Ciencias Exactas Y Naturales, Laboratorio 60, Buenos Aires, Argentina
University of the Western Cape, Biotechnology Department, Private Bag X17, 7535 Bellville, South Africa
Palabras clave:16S; COI; Genetic variation; ITS1; ND1; Speciation; DNA; genetic analysis; genetic variation; lobster; mitochondrial DNA; morphology; speciation (biology); sympatry; taxonomy; Atlantic Ocean; Beagle Channel; Burdwood Bank; Magellan Strait; South America; Crustacea; Decapoda (Crustacea); Galatheidae; Munida gregaria; Munida subrugosa
Año:2008
Volumen:94
Número:2
Página de inicio:421
Página de fin:434
DOI: http://dx.doi.org/10.1111/j.1095-8312.2008.00987.x
Título revista:Biological Journal of the Linnean Society
Título revista abreviado:Biol. J. Linn. Soc.
ISSN:00244066
CODEN:BJLSB
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00244066_v94_n2_p421_PerezBarros.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00244066_v94_n2_p421_PerezBarros

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Citas:

---------- APA ----------
Pérez-Barros, P., D'Amato, M.E., Guzmán, N.V. & Lovrich, G.A. (2008) . Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information. Biological Journal of the Linnean Society, 94(2), 421-434.
http://dx.doi.org/10.1111/j.1095-8312.2008.00987.x
---------- CHICAGO ----------
Pérez-Barros, P., D'Amato, M.E., Guzmán, N.V., Lovrich, G.A. "Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information" . Biological Journal of the Linnean Society 94, no. 2 (2008) : 421-434.
http://dx.doi.org/10.1111/j.1095-8312.2008.00987.x
---------- MLA ----------
Pérez-Barros, P., D'Amato, M.E., Guzmán, N.V., Lovrich, G.A. "Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information" . Biological Journal of the Linnean Society, vol. 94, no. 2, 2008, pp. 421-434.
http://dx.doi.org/10.1111/j.1095-8312.2008.00987.x
---------- VANCOUVER ----------
Pérez-Barros, P., D'Amato, M.E., Guzmán, N.V., Lovrich, G.A. Taxonomic status of two South American sympatric squat lobsters, Munida gregaria and Munida subrugosa (Crustacea: Decapoda: Galatheidae), challenged by DNA sequence information. Biol. J. Linn. Soc. 2008;94(2):421-434.
http://dx.doi.org/10.1111/j.1095-8312.2008.00987.x