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Abstract:

The enzyme 11 βHSD2 protects the non-selective mineralocorticoid receptor from occupation by glucocorticoids in aldosterone target tissues. We studied the effect of stress elicited by intubation with a rubber catheter and administration of 10 ml of 0.45% NaCl (G3), of 10 ml of 200 mM HCl (G4) or intubation alone (G2) on the kinetics of the renal enzyme compared with untreated rats (G1). Microsomes were incubated with increasing masses of 3H corticosterone and 400 μM NAD at pH=7.4 during 5 minutes. Samples were extracted with ethyl acetate and analyzed by TLC. Results for n=4: Vmax for G1, 4.82 ± 0.67. G2, 10.04 ± 0.16. G3, 9.16 ± 0.74. G4, 10.19 ± 0.79 pmoles/min/mg prot. Km for G1, 22.37 ± 2.42. G2, 50.72 ± 7.05*. G3, 55.25 ± 8.37**. G4, 27.40 ± 3.20 nM. (***p<0.001, **p<0.01 and *p<0.05 vs G1). All treatments increased Vmax. Intubation alone and gavage with 0.45% NaCl, but not with 200 mM HCl, increased Km. Taking together, the results could reflect a way to prevent occupation of type I receptors by increased levels of circulating glucocorticoids due to stressful situations. This protection seems more efficient under acidotic conditions causing -in addition to an increased Vmax- a low Km for the enzyme.

Registro:

Documento: Artículo
Título:Activity of renal 11 βhydroxysteroid dehydrogenase 2 (11 βhsd2) in stressed animals
Autor:Igarreta, P.; Calvo, J.C.; Damasco, M.C.
Filiación:PRHOM-CONICET, Lab. de Esteroides, 1428 Buenos Aires, Argentina
Depto. Quimica Biologica, FCEN, U.B.A., 1428 Buenos Aires, Argentina
IBYME, Buenos Aires, Argentina
Dept. Quimica Biologica, Pab. 2 FCEN, U.B.A., Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:11 βHSD2; Acidosis; Corticosteroids; Kidney; Stress; 11beta hydroxysteroid dehydrogenase; aldosterone; corticosterone; glucocorticoid; mineralocorticoid; 11beta hydroxysteroid dehydrogenase; corticosterone; glucocorticoid; hydroxysteroid dehydrogenase; isoenzyme; mineralocorticoid receptor; acidosis; animal experiment; article; binding affinity; data analysis; enzyme activity; kidney function; mammal; nonhuman; rat; receptor binding; species difference; stress; animal; enzymology; kidney; kinetics; male; metabolism; microsome; Sprague Dawley rat; Animalia; Mammalia; 11-beta-Hydroxysteroid Dehydrogenases; Acidosis; Animals; Corticosterone; Glucocorticoids; Hydroxysteroid Dehydrogenases; Isoenzymes; Kidney; Kinetics; Male; Microsomes; Rats; Rats, Sprague-Dawley; Receptors, Mineralocorticoid; Stress
Año:1999
Volumen:64
Número:24
Página de inicio:2285
Página de fin:2290
DOI: http://dx.doi.org/10.1016/S0024-3205(99)00179-4
Título revista:Life Sciences
Título revista abreviado:Life Sci.
ISSN:00243205
CODEN:LIFSA
CAS:11-beta-Hydroxysteroid Dehydrogenases, EC 1.1.1.146; Corticosterone, 50-22-6; Glucocorticoids; Hydroxysteroid Dehydrogenases, EC 1.1.-; Isoenzymes; Receptors, Mineralocorticoid
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v64_n24_p2285_Igarreta

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Citas:

---------- APA ----------
Igarreta, P., Calvo, J.C. & Damasco, M.C. (1999) . Activity of renal 11 βhydroxysteroid dehydrogenase 2 (11 βhsd2) in stressed animals. Life Sciences, 64(24), 2285-2290.
http://dx.doi.org/10.1016/S0024-3205(99)00179-4
---------- CHICAGO ----------
Igarreta, P., Calvo, J.C., Damasco, M.C. "Activity of renal 11 βhydroxysteroid dehydrogenase 2 (11 βhsd2) in stressed animals" . Life Sciences 64, no. 24 (1999) : 2285-2290.
http://dx.doi.org/10.1016/S0024-3205(99)00179-4
---------- MLA ----------
Igarreta, P., Calvo, J.C., Damasco, M.C. "Activity of renal 11 βhydroxysteroid dehydrogenase 2 (11 βhsd2) in stressed animals" . Life Sciences, vol. 64, no. 24, 1999, pp. 2285-2290.
http://dx.doi.org/10.1016/S0024-3205(99)00179-4
---------- VANCOUVER ----------
Igarreta, P., Calvo, J.C., Damasco, M.C. Activity of renal 11 βhydroxysteroid dehydrogenase 2 (11 βhsd2) in stressed animals. Life Sci. 1999;64(24):2285-2290.
http://dx.doi.org/10.1016/S0024-3205(99)00179-4