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Abstract:

The purpose of this work is to review stability and activation properties of type 1 receptor, in order to explain the reasons for its extreme in vitro instability. We demonstrate that the treatment of rat kidney cytosol with H2O2 prevents aldosterone binding, DNA/steroid- receptor complex interactions, and prevents the receptor thermal inactivation. In contrast, exogenous sulfhydryl reducing reagents are necessary to insure maximum binding of mineralocorticoid receptor and DNA/steroid-receptor interaction. However, the presence of β- mercaptoethanol in thermal induced incubations reverts the H2O2 protection. We also demonstrate that contaminations with free or sequestered iron are harmful for both, receptor binding capacity (in a reversible form) and for hormone-receptor/DNA binding properties (in a partially reversible form). We propose a sulfhydryl oxidative mechanism for type I mineralocorticoid receptor inactivation in which iron contaminants might accelerate this process by oxidative catalysis. We also demonstrate that when thiol groups are blocked by specific reagents such as N-ethyl-maleimide or dithionitrobenzoic acid, type I sites loose binding capacity, but the protein is protected from oxidation as well as inactivation.

Registro:

Documento: Artículo
Título:Stability study on renal type I mineralocorticoid receptor
Autor:Galigniana, M.D.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires (1428), Argentina
Palabras clave:mineralocorticoid receptor; oxidation; stability; thiol groups; 5,5' dithiobis(2 nitrobenzoic acid); aldosterone; DNA; edetic acid; egtazic acid; hydrogen peroxide; iron; mercaptoethanol; mineralocorticoid receptor; n ethylmaleimide; steroid; thiol group; animal cell; article; cell free system; cytosol; DNA binding; high temperature procedures; kidney; nonhuman; oxidation; rat; receptor binding; thermostability; Animalia
Año:1996
Volumen:59
Número:7
Página de inicio:511
Página de fin:521
DOI: http://dx.doi.org/10.1016/0024-3205(96)00331-1
Título revista:Life Sciences
Título revista abreviado:LIFE SCI.
ISSN:00243205
CODEN:LIFSA
CAS:5,5' dithiobis(2 nitrobenzoic acid), 69-78-3; aldosterone, 52-39-1, 6251-69-0; DNA, 9007-49-2; edetic acid, 150-43-6, 60-00-4; egtazic acid, 67-42-5; hydrogen peroxide, 7722-84-1; iron, 14093-02-8, 53858-86-9, 7439-89-6; mercaptoethanol, 60-24-2; n ethylmaleimide, 128-53-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v59_n7_p511_Galigniana

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Citas:

---------- APA ----------
(1996) . Stability study on renal type I mineralocorticoid receptor. Life Sciences, 59(7), 511-521.
http://dx.doi.org/10.1016/0024-3205(96)00331-1
---------- CHICAGO ----------
Galigniana, M.D. "Stability study on renal type I mineralocorticoid receptor" . Life Sciences 59, no. 7 (1996) : 511-521.
http://dx.doi.org/10.1016/0024-3205(96)00331-1
---------- MLA ----------
Galigniana, M.D. "Stability study on renal type I mineralocorticoid receptor" . Life Sciences, vol. 59, no. 7, 1996, pp. 511-521.
http://dx.doi.org/10.1016/0024-3205(96)00331-1
---------- VANCOUVER ----------
Galigniana, M.D. Stability study on renal type I mineralocorticoid receptor. LIFE SCI. 1996;59(7):511-521.
http://dx.doi.org/10.1016/0024-3205(96)00331-1