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Abstract:

Muscarinic receptors were assessed by [3H]-quinuclidinyl benzilate (QNB) binding in 900 xg supernatants of bovine superior cervical ganglia (SCG). At 30°C half maximal binding was reached within 3 min and equilibrium within 30 min. Scatchard analysis revealed a single population of binding sites with dissociation constant (Kd)= 0.15 ± 0.01 nM and site concentration (Bmax)= 101 ± 4 fmoles/mg prot. Binding was specific for muscarinic drugs. Incubation of bovine SCG with different hormones (10-7M) indicated that LH, TRH and testosterone depressed significantly Bmax, and that prolactin decreased both Kd and Bmax of [3H]-QNB binding. Several other hormones tested (TSH, GH, FSH, LHRH, angiotensin II, bradykinin, melatonin, estradiol, thyroxine and triiodothyronine) did not affect QNB binding. Hormone effects were not due to a direct interference with radioligand binding to membrane. The injection of LH to orchidectomized rats depressed Bmax of SCG QNB binding without changing the Kd. These results suggest that muscarinic cholinergic neurotransmission in SCG may be affected by hormones. © 1983.

Registro:

Documento: Artículo
Título:Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia
Autor:Gejman, P.V.; Cardinali, D.P.
Filiación:Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN). Serrano 665/669, 1414 Buenos Aires, Argentina
Palabras clave:angiotensin; bradykinin; estradiol; follitropin; gonadorelin; growth hormone; liothyronine; luteinizing hormone; muscarinic receptor; prolactin; protirelin; radioisotope; testosterone; thyrotropin; thyroxine; animal cell; autonomic nervous system; cattle; drug efficacy; drug receptor binding; nonhuman; pharmacokinetics; quinuclidine derivative h 3; quinuclidinyl benzilate h 3; rat; superior cervical ganglion; Animal; Cattle; Female; Ganglia, Sympathetic; Hormones; Luteinizing Hormone; Male; Prolactin; Protirelin; Quinuclidinyl Benzilate; Rats; Rats, Inbred Strains; Receptors, Cholinergic; Receptors, Muscarinic; Support, Non-U.S. Gov't; Testosterone
Año:1983
Volumen:32
Número:9
Página de inicio:965
Página de fin:972
DOI: http://dx.doi.org/10.1016/0024-3205(83)90926-8
Título revista:Life Sciences
Título revista abreviado:Life Sci.
ISSN:00243205
CODEN:LIFSA
CAS:angiotensin, 11128-99-7, 1407-47-2; bradykinin, 58-82-2, 5979-11-3; estradiol, 50-28-2; follitropin, 9002-68-0; gonadorelin, 33515-09-2, 9034-40-6; growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; liothyronine, 6138-47-2, 6893-02-3; luteinizing hormone, 39341-83-8, 9002-67-9; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; protirelin, 24305-27-9; testosterone, 58-22-0; thyrotropin, 9002-71-5; thyroxine, 7488-70-2; Hormones; Luteinizing Hormone, 9002-67-9; Prolactin, 9002-62-4; Protirelin, 24305-27-9; Quinuclidinyl Benzilate, 6581-06-2; Receptors, Cholinergic; Receptors, Muscarinic; Testosterone, 57-85-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v32_n9_p965_Gejman

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Citas:

---------- APA ----------
Gejman, P.V. & Cardinali, D.P. (1983) . Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia. Life Sciences, 32(9), 965-972.
http://dx.doi.org/10.1016/0024-3205(83)90926-8
---------- CHICAGO ----------
Gejman, P.V., Cardinali, D.P. "Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia" . Life Sciences 32, no. 9 (1983) : 965-972.
http://dx.doi.org/10.1016/0024-3205(83)90926-8
---------- MLA ----------
Gejman, P.V., Cardinali, D.P. "Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia" . Life Sciences, vol. 32, no. 9, 1983, pp. 965-972.
http://dx.doi.org/10.1016/0024-3205(83)90926-8
---------- VANCOUVER ----------
Gejman, P.V., Cardinali, D.P. Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia. Life Sci. 1983;32(9):965-972.
http://dx.doi.org/10.1016/0024-3205(83)90926-8