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Abstract:

Flow cytometry (FCM) is a powerful tool for analyzing physiological characteristics of microorganisms on a single-cell basis and identifying heterogeneities within population. This work analyzed the UV-C induced damage on Escherichia coli ATCC 11229; Listeria innocua ATCC 33090 and Saccharomyces cerevisiae KE162 cells by applying flow cytometry technique. The UV-C doses, obtained by altering the exposure time and measured by the iodide-iodate chemical actinometer, ranged between 0 and 5 kJ/m2. E. coli; L. innocua and S. cerevisiae populations were quantified by plate count technique. For flow cytometry studies, cells were labeled with fluorescein diacetate (FDA) for detecting membrane integrity and esterase activity, and with propidium iodide (PI) for monitoring membrane integrity. The results showed that mechanisms of cellular damage differed according to time of exposure to ultraviolet radiation and the organism tested. E. coli and S. cerevisiae sub-populations with PI increased within the first minutes of UV-C treatment, without much change afterwards. On the contrary, FCM was used to detect the inactivation of those L. innocua sub-populations of viable microorganisms (maintaining metabolic activity) which were non-culturable due to membrane rupture and thus not detectable by viable plate count technique. © 2010 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
Autor:Schenk, M.; Raffellini, S.; Guerrero, S.; Blanco, G.A.; Alzamora, S.M.
Filiación:Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, (1428) C.A.B.A., Argentina
Departamento de Tecnología, Universidad Nacional de Lujan, Ruta Nacional 5 y 7, (6700) Luján, Buenos Aires, Argentina
Member of Consejo Nacional de Investigaciones Científicas y Técnicas de la Argentina, Argentina
Instituto de Estudios de Inmunidad Humoral (IDEHU)- CONICET, Junín 956, (1113) C.A.B.A., Argentina
Palabras clave:E. coli; Flow cytometry; Inactivation; L. innocua; S. cerevisiae; UV-C light; E. coli; Inactivation; L. innocua; S.cerevisiae; UV-C light; Diseases; Flow cytometry; Listeria; Meteorological instruments; Microorganisms; Ultraviolet radiation; Yeast; Escherichia coli; Escherichia coli; Listeria innocua; Saccharomyces cerevisiae
Año:2011
Volumen:44
Número:1
Página de inicio:191
Página de fin:198
DOI: http://dx.doi.org/10.1016/j.lwt.2010.05.012
Título revista:LWT - Food Science and Technology
Título revista abreviado:LWT - Food Sci. Technol.
ISSN:00236438
CODEN:LBWTA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236438_v44_n1_p191_Schenk

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Citas:

---------- APA ----------
Schenk, M., Raffellini, S., Guerrero, S., Blanco, G.A. & Alzamora, S.M. (2011) . Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry. LWT - Food Science and Technology, 44(1), 191-198.
http://dx.doi.org/10.1016/j.lwt.2010.05.012
---------- CHICAGO ----------
Schenk, M., Raffellini, S., Guerrero, S., Blanco, G.A., Alzamora, S.M. "Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry" . LWT - Food Science and Technology 44, no. 1 (2011) : 191-198.
http://dx.doi.org/10.1016/j.lwt.2010.05.012
---------- MLA ----------
Schenk, M., Raffellini, S., Guerrero, S., Blanco, G.A., Alzamora, S.M. "Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry" . LWT - Food Science and Technology, vol. 44, no. 1, 2011, pp. 191-198.
http://dx.doi.org/10.1016/j.lwt.2010.05.012
---------- VANCOUVER ----------
Schenk, M., Raffellini, S., Guerrero, S., Blanco, G.A., Alzamora, S.M. Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry. LWT - Food Sci. Technol. 2011;44(1):191-198.
http://dx.doi.org/10.1016/j.lwt.2010.05.012