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Abstract:

The effect of enzymatic modification of wheat flour proteins on their functional properties was evaluated as a function of the degree of hydrolysis (DH). Soluble protein increased from 7.1 to 53% for 0% to 36.7% DH flour, and viscosity and yield values decreased concomitantly. Emulsion capacity slightly decreased up to 14% DH and then increased. Baummann water and oil absorption capacities and farinograph water absorption had similar trends; they showed a maximum increase at 3.8% DH where Baumann and farinograph absorption values increased by 30%. Limited degree of hydrolysis (3.8-14.1% DH) was sufficient to improve quality parameters of cakes such as volume, moisture and crumb colour. Added protease resulted in pronounced reduction in molecular mass distribution of wheat proteins, especially glutenins. The increase in water soluble protein as hydrolysis proceeded was related to proteins of molecular mass less than 25 kDa. The reduction in viscosity and foam stability was related to the extent of hydrolysis of high molecular mass glutenin protein. The increased Baumann and farinograph water absorption by limited hydrolysis up to 3.8-5.4% DH may be related to increased number of polar sites and flexibility of protein network, without extensive degradation of glutenins. © 1997 Academic Press Limited.

Registro:

Documento: Artículo
Título:Functional Properties of Protease Modified Wheat Flours
Autor:Bombara, N.; Añón, M.C.; Pilosof, A.M.R.
Filiación:Departamento de Industrias, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, (1428), Buenos Aires, Argentina
Ctro. Invest. y Desarrollo C., 47 y 116, (1900), La Plata, Argentina
Palabras clave:Emulsion capacity; Protease hydrolysis; Viscosity; Wheat flour; Triticum aestivum
Año:1997
Volumen:30
Número:5
Página de inicio:441
Página de fin:447
DOI: http://dx.doi.org/10.1006/fstl.1996.0197
Título revista:LWT - Food Science and Technology
Título revista abreviado:LWT - Food Sci. Technol.
ISSN:00236438
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00236438_v30_n5_p441_Bombara

Referencias:

  • Sproessler, B.G., Milling and baking (1993) Enzymes in Food Processing, , New York: Academic Press
  • Lyoons, T.P., Proteinase enzymes relevant to the baking industry (1982) Biochemical Society Transactions, 10, pp. 287-290
  • Matz, S.A., Matz, T.D., (1978) Cookie and Cracker Technology, , Westport: Avi Publications Co
  • Pomeranz, Y., Rubenthaler, G.L., Finey, K.F., Evaluation of the effects of proteolytic enzymes on bread flour properties (1966) Food Technology, 20, pp. 95-98
  • Wu, J.Y., Hoseney, R.C., Rheological changes in cracker sponges during an 18-hour fermentation (1989) Cereal Chemistry, 66, pp. 182-185
  • Bombara, N., Pilosof, A.M.R., Añón, M.C., Mathematical model for formation rate and collapse of foams from enzyme modified wheat flours (1994) Journal of Food Science, 59, pp. 626-628
  • Bombara, N., Pilosof, A.M.R., Añón, M.C., Kinetics of wheat proteins solubilization with a fungal protease (1992) Lebensmittel-Wissenschaft und -Technologie, 25, pp. 527-531
  • Iglesias, H.A., Chirife, J., Lombardi, J.L., An equation for correlating equilibrium moisture content in foods (1975) Journal of Food Technology, 10, pp. 289-297
  • Kanterewicz, R.J., Elizalde, B.E., Pilosof, A.M.R., Bartholomai, G.B., Water-oil absorption index (WOAI): A simple method for predicting the emulsifying capacity of food proteins (1987) Journal of Food Science, 52, pp. 1381-1383
  • Webb, N.B., Ivey, F.J., Craig, H.B., Jones, V.A., Monroe, R.J., The measurement of emulsifying capacity by electrical resistance (1970) Journal of Food Science, 35, pp. 501-504
  • Bottomley, R.C., Kearns, H.F., Schofield, J.D., Characterisation of wheat flour and gluten proteins using buffers containing sodium dodecyl sulphate (1982) Journal of the Science of Food and Agriculture, 33, pp. 481-491
  • Bradford, M.M., A rapid and sensitive method for the quantitative of microgram quantities of protein utilizing the principle of protein dye-binding (1976) Analytical Biochemistry, 72, pp. 248-254
  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
  • (1983) Approved Methods of the AACC, , St Paul: The Association
  • Yamazaki, W.T., White layer cake test (1970) Cereal Science Today, 15, pp. 262-265
  • Hoseney, R.C., Wheat gluten: Rheological and gas retaining properties (1991) Water Relationships in Food, , New York: Plenum Press
  • Attenburrow, G., Barnes, D.J., Davies, A.P., Ingman, S.J., Rheological properties of wheat gluten (1990) Cereal Science, 12, pp. 1-14
  • Weegels, P.L., De Jager, A., Hamer, R.J., Enzymatic modification of wheat gluten. Part I. Screening of proteases (1990) Gluten Proteins, , W. Bushuk, & R. Tkachuk. St Paul: American Association of Cereal Chemistry
  • Fox, P.F., Morrissey, P.A., Mulvihill, D.M., Chemical and enzymatic modification of food proteins (1982) Development in Food Proteins, , B.J.F. Hudson. London: Elsevier Applied Science Publishers LTD
  • Puski, G., Modification of functional properties of soy proteins by proteolytic enzyme treatment (1975) Cereal Chemistry, 52, pp. 655-664
  • Adler-Nissen, J., Olsen, H.S., The influence of peptide chain length on taste and functional properties of enzymatically modified soy protein (1979) American Chemical Society Symposium Series, 92, pp. 125-146
  • Bernardi, L.S., Pilosof, A.M.R., Bartholomai, G.B., Enzymatic modification of soy protein concentrates by fungal and bacterial proteases (1991) Journal of the American Oil Chemists Society, 68, pp. 102-105
  • Hermansson, A.M., Olsson, D., Holmberg, B., Functional properties of proteins for foods. Modification studies on rapeseed protein concentrate (1974) Lebensmitel Wissenschaft Und-technologie, 7, pp. 176-181
  • Hevia, P., Whitaker, J.R., Olcott, H.J., Solubilization of a fish protein concentrate with proteolytic enzymes (1976) Journal of Agriculture and Food Chemistry, 24, pp. 383-385
  • Batey, I.L., Enzymatic solubilization of wheat gluten (1986) Journal of Applied Biochemistry, 7, pp. 423-429
  • Fox, P.F., Mulvihill, D.M., Enzymes in wheat, flour and bread (1982) Advances in Cereal Science and Technology, , V.Y. Pomeranz. St Paul: American Association of Cereal Chemists
  • Weegels, P.L., Voorpostel, A.M.B., (1990) Enzymatic Modification of Wheat Gluten. Part II. Functional Properties of the Digests, , W. Bushuk, & R. Tkachuk. St Paul: American Association of Cereal Chemistry
  • Urbanski, G.E., Wie, L.S., Nelson, A.I., Steinberg, H.P., Flour characteristics of soybean constituents controlled by ratio of total to imbibed water (1983) Journal of Food Science, 48, pp. 691-694
  • Khan, K., Bushuk, W., Structure of wheat gluten in relation to functionality in breadmaking (1979) Functionality and Protein Structure, , El A. Pour. Washington: American Chemical Society
  • Mac Ritchie, F., Evaluation of contributions from wheat protein fractions to dough mixing and breadmaking (1987) Journal of Cereal Science, 6, pp. 259-268
  • Rasper, V.F., De Man, J.M., Measurement of hydration capacity of wheat flour/starch mixtures (1980) Cereal Chemistry, 57, pp. 27-31
  • Hoseney, R.C., Physical chemistry of bread dough (1992) Physical Chemistry of Foods, , H.G. Schwartzberg, & R.W. Hartel. New York: Marcel Dekker Inc
  • Adler-Nissen, J., A review of food protein hydrolysis (1986) Enzymic Hydrolysis of Food Proteins, , J. Adler-Nissen. New York: Elsevier Applied Science Publishers

Citas:

---------- APA ----------
Bombara, N., Añón, M.C. & Pilosof, A.M.R. (1997) . Functional Properties of Protease Modified Wheat Flours. LWT - Food Science and Technology, 30(5), 441-447.
http://dx.doi.org/10.1006/fstl.1996.0197
---------- CHICAGO ----------
Bombara, N., Añón, M.C., Pilosof, A.M.R. "Functional Properties of Protease Modified Wheat Flours" . LWT - Food Science and Technology 30, no. 5 (1997) : 441-447.
http://dx.doi.org/10.1006/fstl.1996.0197
---------- MLA ----------
Bombara, N., Añón, M.C., Pilosof, A.M.R. "Functional Properties of Protease Modified Wheat Flours" . LWT - Food Science and Technology, vol. 30, no. 5, 1997, pp. 441-447.
http://dx.doi.org/10.1006/fstl.1996.0197
---------- VANCOUVER ----------
Bombara, N., Añón, M.C., Pilosof, A.M.R. Functional Properties of Protease Modified Wheat Flours. LWT - Food Sci. Technol. 1997;30(5):441-447.
http://dx.doi.org/10.1006/fstl.1996.0197