Artículo
Abstract:
The use of feces for population size assessment and diet analysis of carnivore species depends on accurate feces identification. Fecal bile acid composition appears to be species among carnivores and therefore a useful method to identify fecal samples. To help identify 194 field-collected feces of cougars (Puma concolor) and jaguars (Felis onca), 2 sympatric carnivore species of the Chaco Regia (Paraguay), we developed a method to determine feces origin using thin layer chromatography (TLC) bile acid patterns. Bile acids were extracted from 11 fecal samples from cougars and 10 from jaguars from different zoological gardens. Although cougar and jaguar feces contain the same identifiable bile acids, feces can be distinguished by the presence of a single unknown bile acid present consistently only among jaguar feces. The use of this bile acid as an indicator of feces origin, evaluated by means of 2 blind tests of 10 samples each, reached 80%. We also compared our results with a previous identification of the field collected feces based on physical characteristics. We suggest that TLC analysis of fecal bile acids is the best identification technique of cougar and jaguar feces technique available.
Registro:
| Documento: | Artículo |
| Título: | Identification of cougar and jaguar feces through bile acid chromatography |
| Autor: | Fernández, G.J.; Corley, J.C.; Capurro, A.F. |
| Filiación: | Departamento de Biología, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, (1426) Buenos Aires, Argentina Departamento de Investigación, Universidad de Belgrano, Zabala 1851, (1426) Buenos Aires, Argentina Inst. de Biol. y Med. Experimental, CONICET, Vuelta de Obligado 2490, (1428) Capital Federal, Argentina |
| Palabras clave: | Chaco Region; Chromatography; Cougar; Fecal bile acids; Feces; Felis onca; Jaguar; Paraguay; Puma concolor; Thinlayer; Chaco; Cyamopsis tetragonoloba; Felis; Panthera onca; Puma concolor |
| Año: | 1997 |
| Volumen: | 61 |
| Número: | 2 |
| Página de inicio: | 506 |
| Página de fin: | 510 |
| DOI: | http://dx.doi.org/10.2307/3802608 |
| Título revista: | Journal of Wildlife Management |
| Título revista abreviado: | J. WILDL. MANAGE. |
| ISSN: | 0022541X |
| CODEN: | JWMAA |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022541X_v61_n2_p506_Fernandez |
Referencias:
- Aldred, D.R., (1980) Biochemical Identification of Carnivore Scats, 16p. , M.S. Thesis. Mississipi State Univ., Missisipi State
- Asa, C.S., Wallace, M.P., Diet and activity pattern of the Sechurian desert fox (Dusicyon sechurae) (1990) J. Mammal., 71, pp. 69-72
- Clinite, E.W., (1981) Biochemical Analysis of Mountain Lion and Bobcat Scats: Difference between Species and Sex, 49p. , M.S. Thesis. San Jose State Univ., San José , Calif
- Capurro, A.F., Novaro, A.J., Travaini, A., Romero, M., Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores (1997) J. Wildl. Manage., , In Press
- Daner, D.A., Dodd, N., Comparison of coyote and grey fox scat diameters (1982) J. Wildl. Manage., 46, pp. 240-241
- Doncaster, C.P., Dickmen, C.R., Mac Donald, D.W., Feeding ecology of red fox (Vulpes vulpes) in the city of Oxford, England (1990) J. Mammal., 71, pp. 188-194
- Duran, J.C., Poiani, A., Raedeke, K., Food habits of Dusicyon griseus in Northern Chile (1984) J. Mammal., 65, pp. 515-517
- Jaksic, F.M., Yañez, J.L., Ran, J.R., Trophic relation of the southernmost populations of Dusicyon in Chile (1983) J. Mammal., 64, pp. 693-697
- Johnson, M.K., Aldred, D.R., Clinite, E.W., Kutilek, M.J., Biochemical identification of bobcat scats (1981) Proc. Bobcat Res. Conf., Nat. Wild. Fed. Sci. and Tech. Ser., 6, pp. 92-96
- Belden, R.C., Aldred, D.R., Differentiation of mountain lion and bobcat scats (1984) J. Wildl. Manage., 48, pp. 239-244
- Clark, T.W., Schroeder, M.H., Richardson, L., Fecal bile acids of black-footed ferrets (1986) Great Basin Nat., 8, pp. 141-144
- Kritchevsky, D., Martak, D.S., Rothblat, G.H., Detection of bile acids in thin-layer chromatography (1963) Anal. Biochem., 5, pp. 388-392
- Major, M., Johnson, M.K., Davis, W.S., Kellogg, T.F., Identifying scats by recovery of bile acids (1980) J. Wildl. Manage., 44, pp. 290-293
- Quinn, T., Jackman, W.R., Influence of diet on detection of fecal bile acids by thin-layer chromatography (1994) J. Wildl. Manage., 58, pp. 295-299
- Weaver, J.L., Fritz, S.H., Comparison of coyote and wolf scat diameters (1979) J. Wildl. Manage., 43, pp. 786-788
- Windberg, L.A., Mitchell, C.D., Winter diets of coyotes in relation to prey abundance in southern Texas (1990) J. Mammal., 71, pp. 439-447
Citas:
---------- APA ----------
Fernández, G.J., Corley, J.C. & Capurro, A.F. (1997) . Identification of cougar and jaguar feces through bile acid chromatography. Journal of Wildlife Management, 61(2), 506-510.http://dx.doi.org/10.2307/3802608
---------- CHICAGO ----------
Fernández, G.J., Corley, J.C., Capurro, A.F. "Identification of cougar and jaguar feces through bile acid chromatography" . Journal of Wildlife Management 61, no. 2 (1997) : 506-510.http://dx.doi.org/10.2307/3802608
---------- MLA ----------
Fernández, G.J., Corley, J.C., Capurro, A.F. "Identification of cougar and jaguar feces through bile acid chromatography" . Journal of Wildlife Management, vol. 61, no. 2, 1997, pp. 506-510.http://dx.doi.org/10.2307/3802608
---------- VANCOUVER ----------
Fernández, G.J., Corley, J.C., Capurro, A.F. Identification of cougar and jaguar feces through bile acid chromatography. J. WILDL. MANAGE. 1997;61(2):506-510.http://dx.doi.org/10.2307/3802608
