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Abstract:

The p24 protein, one of the three proteins implicated in local movement of potato virus X (PVX), was expressed in transgenic tobacco plants (Nicotiana tabacum Xanthi D8 NN). Plants with the highest level of p24 accumulation exhibited a stunted and slightly chlorotic phenotype. These transgenic plants facilitate the cell-to-cell movement of a mutant of PVX that contained a frameshift mutation in p24. Upon inoculation with tobacco mosaic virus (TMV), the size of necrotic local lesions was significantly smaller in p24+ plants than in nontransgenic, control plants. Systemic resistance to tobamoviruses was also evidenced after inoculation of p24+ plants with Ob, a virus that evades the hypersensitive response provided by the N gene. In the latter case, no systemic symptoms were observed, and virus accumulation remained low or undetectable by Western immunoblot analysis and back-inoculation assays. In contrast, no differences were observed in virus accumulation after inoculation with PVX, although more severe symptoms were evident on p24-expressing plants than on control plants. Similarly, infection assays conducted with potato virus Y showed no differences between control and transgenic plants. On the other hand, a considerable delay in virus accumulation and symptom development was observed when transgenic tobacco plants containing the movement protein (MP) of TMV were inoculated with PVX. Finally, a movement defective mutant of TMV was inoculated on p24+ plants or in mixed infections with PVX on nontransgenic plants. Both types of assays failed to produce TMV infections, implying that TMV MP is not interchangeable with the PVX MPs.

Registro:

Documento: Artículo
Título:Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses
Autor:Ares, X.; Calamante, G.; Cabral, S.; Lodge, J.; Hemenway, P.; Beachy, R.N.; Mentaberry, A.
Filiación:Inst. Invest. Ing. Genet. y Biol. M., Fac. de Ciencias Exactas y Naturales, UBA, Buenos Aires 1428, Argentina
Dept. of Molec. Biol. and Pharmacol., Washington University, School of Medicine, St. Louis, MO 63110, United States
Department of Biochemistry, North Carolina State University, Raleigh, NC 27695, United States
Department of Cell Biology, Scripps Research Institute, San Diego, CA 92037, United States
INGEBI-CONICET, Vuelta de Obligado 2490, 1428 Capital Federal, Argentina
Palabras clave:gag protein; article; controlled study; frameshift mutation; nonhuman; priority journal; tobacco mosaic virus; transgenic plant; virus mutation; virus resistance; Animals; Antibodies, Viral; Base Sequence; DNA Primers; Frameshift Mutation; Gene Expression; Genetic Complementation Test; Movement; Open Reading Frames; Phenotype; Plants, Genetically Modified; Plants, Toxic; Potexvirus; Rabbits; Tobacco; Tobacco Mosaic Virus; Tobamovirus; Viral Proteins
Año:1998
Volumen:72
Número:1
Página de inicio:731
Página de fin:738
Título revista:Journal of Virology
Título revista abreviado:J. Virol.
ISSN:0022538X
CODEN:JOVIA
CAS:Antibodies, Viral; DNA Primers; Viral Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022538X_v72_n1_p731_Ares

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Citas:

---------- APA ----------
Ares, X., Calamante, G., Cabral, S., Lodge, J., Hemenway, P., Beachy, R.N. & Mentaberry, A. (1998) . Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses. Journal of Virology, 72(1), 731-738.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022538X_v72_n1_p731_Ares [ ]
---------- CHICAGO ----------
Ares, X., Calamante, G., Cabral, S., Lodge, J., Hemenway, P., Beachy, R.N., et al. "Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses" . Journal of Virology 72, no. 1 (1998) : 731-738.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022538X_v72_n1_p731_Ares [ ]
---------- MLA ----------
Ares, X., Calamante, G., Cabral, S., Lodge, J., Hemenway, P., Beachy, R.N., et al. "Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses" . Journal of Virology, vol. 72, no. 1, 1998, pp. 731-738.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022538X_v72_n1_p731_Ares [ ]
---------- VANCOUVER ----------
Ares, X., Calamante, G., Cabral, S., Lodge, J., Hemenway, P., Beachy, R.N., et al. Transgenic plants expressing potato virus X ORF2 protein (p24) are resistant to tobacco mosaic virus and Ob tobamoviruses. J. Virol. 1998;72(1):731-738.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022538X_v72_n1_p731_Ares [ ]