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Abstract:

A theoretical model of the coupling between conformational fluctuation of the protein matrix and enzyme catalysis is here proposed. The model rests on the following assumptions: (1) The active site of the enzyme molecule can exist in at least two thermodynamically stable states: open and closed; (2) only the open conformation exchange substrates with the medium; (3) the enzyme in the closed conformation acquired over its substrate the maximal catalytic activity, (4) the fluctuation of the enzyme molecule between both conformations is an absolute requirement for the completion of a catalytical cycle. The parallelism of this cyclic catalytic process with phase transfer catalysis is discussed. Positive and negative cooperativity due to the coupling of conformational fluctuation in oligomers is predicted by the model. The same is true for half of the sites reactivity. Other kinetic features of the model are discussed. © 1982.

Registro:

Documento: Artículo
Título:Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis?
Autor:Olavarría, J.
Filiación:Instituto de Investigations Bioquimicas Fundación Campomar, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:enzyme; allosterism; article; binding site; biological model; catalysis; kinetics; metabolism; protein conformation; Allosteric Regulation; Binding Sites; Catalysis; Enzymes; Kinetics; Models, Biological; Protein Conformation
Año:1982
Volumen:99
Número:1
Página de inicio:21
Página de fin:30
DOI: http://dx.doi.org/10.1016/0022-5193(82)90387-3
Título revista:Journal of Theoretical Biology
Título revista abreviado:J. Theor. Biol.
ISSN:00225193
CODEN:JTBIA
CAS:Enzymes
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00225193_v99_n1_p21_Olavarria

Referencias:

  • Banks, Blake, Evans, Haser, Rice, Hardy, Herrett, Phillips, (1979) Nature, 279, p. 774
  • Beece, Eisenstein, Frauenfelder, Good, Marden, Reinisch, Reynolds, Yue, (1980) Biochemistry, 19, p. 5147
  • Bender, Komiyama, (1977) Biorganic Chemistry, 1. , chapter 2, E.E. van Tamelen, Academic Press, New York
  • Bennett, Steitz, (1978) Proc. natn Acad. Sci. U.S.A, 75, p. 4848
  • Bennett, Steitz, (1980) J. mol. Biol, 140, p. 211
  • Blumenfeld, (1976) J. theor. Biol, 58, p. 269
  • Careri, Fasella, Gratton, (1979) Ann. Rev. Biophys. Bioeng, 8, p. 69
  • Caserta, Cervigni, (1974) Proc. natn Acad. Sci. U.S.A, 71, p. 4421
  • Cleland, (1975) Acc. Chem. Res, 8, p. 145
  • Cohen, Vaidya, Schultz, Active Site of  -Chymotrypsin Activation by Association-Desolvation (1970) Proceedings of the National Academy of Sciences, 66, p. 249
  • Dehmlow, Phase-Transfer Catalyzed Two-Phase Reactions in Preparative Organic Chemistry (1974) Angewandte Chemie International Edition in English, 13, p. 170
  • Eklund, Samama, Wallen, Branden, Akeson, Jones, (1981) J. mol. Biol, 146, p. 561
  • Evans, Hudson, (1979) Nature, 279, p. 500
  • Gavish, (1978) Biophys. Struct. Mechanism, 4, p. 37
  • Gavish, Weber, (1979) Biochemistry, 18, p. 1269
  • Green, Ji, (1972) Proc. natn Acad. Sci. U.S.A, 69, p. 726
  • Green, A FRAMEWORK OF PRINCIPLES FOR THE UNIFICATION OF BIOENERGETICS* (1974) Annals of the New York Academy of Sciences, 227, p. 6
  • Herriott, Picker, (1975) J. Am. Chem. Soc, 97, p. 2345
  • Jencks, (1975) Adv. Enzymology, 43, p. 219
  • Johnson, Andree, Fisher, (1981) J. biol. Chem, 256, p. 6381
  • Koshland, Jr., Application of a Theory of Enzyme Specificity to Protein Synthesis (1958) Proceedings of the National Academy of Sciences, 44, p. 98
  • Koshland, Jr., (1959) The Enzymes, 1, pp. 305-346. , 2nd edition, P.D. Boyer, H. Lardy, K. Myrback, Academic Press, New York
  • Koshland, Jr., Conformational Aspects of Enzyme Regulation (1969) Curr. Top. Cell. Regul, 1, p. 1
  • Koshland, Jr., Nemethy, Filmer, (1966) Biochemistry, 5, p. 365
  • Kunitake, (1977) Biorganic Chemistry, 1. , E.E. van Tamelen, Academic Press, New York, chapter 8
  • Laurent, Obrink, (1972) Eur. J. Biochem, 28, p. 94
  • Lazdunski, Flip-Flop Mechanisms and Half-Site Enzymes (1972) Curr. Top. Cell. Regul, 6, p. 267
  • Low, Somero, (1975) Proc. natn Acad. Sci. U.S.A, 72, p. 3014
  • Matko, Tron, Balazs, Hevessy, Somogyi, Damjanovich, (1980) Biochemistry, 19, p. 5782
  • McCammon, Gelin, Karplus, Wolynes, (1976) Nature, 262, p. 325
  • Monod, Wyman, Changeaux, On the nature of allosteric transitions: A plausible model (1966) Journal of Molecular Biology, 12, p. 88
  • Pai, Sachsenhaimer, Schirmer, Schultz, (1977) J. mol. Biol, 114, p. 37
  • Perutz, Concluding Remarks (1967) Proceedings of the Royal Society B: Biological Sciences, 167, p. 448
  • Reuben, (1971) Proc. natn Acad. Sci. U.S.A, 68, p. 563
  • Starks, (1971) J. Am. Chem. Soc, 93, p. 195
  • Warshel, (1978) Proc. natn Acad. Sci. U.S.A, 75, p. 5250

Citas:

---------- APA ----------
(1982) . Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis?. Journal of Theoretical Biology, 99(1), 21-30.
http://dx.doi.org/10.1016/0022-5193(82)90387-3
---------- CHICAGO ----------
Olavarría, J. "Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis?" . Journal of Theoretical Biology 99, no. 1 (1982) : 21-30.
http://dx.doi.org/10.1016/0022-5193(82)90387-3
---------- MLA ----------
Olavarría, J. "Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis?" . Journal of Theoretical Biology, vol. 99, no. 1, 1982, pp. 21-30.
http://dx.doi.org/10.1016/0022-5193(82)90387-3
---------- VANCOUVER ----------
Olavarría, J. Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis?. J. Theor. Biol. 1982;99(1):21-30.
http://dx.doi.org/10.1016/0022-5193(82)90387-3