Artículo

González Pereyra, M.L.; Chiacchiera, S.M.; Rosa, C.A.; Sager, R.; Dalcero, A.M.; Cavaglieri, L. "Comparative analysis of the mycobiota and mycotoxins contaminating corn trench silos and silo bags" (2011) Journal of the Science of Food and Agriculture. 91(8):1474-1481
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Abstract:

Silage is one of the most important feed sources for bovines. Mycotoxin contamination of feedstuffs is a worldwide concern. The aim of this study was to compare mycobiota and levels of aflatoxin B 1 (AFB 1), fumonisin B 1 (FB 1), deoxynivalenol (DON), zearalenone (ZEA) and patulin (PAT) in corn trench silos and silo bags. RESULTS: Dry matter was higher in trench silos. Counts varied from not detected to 10 8 CFU g -1 in both trench silos and silo bags. Isolation frequencies of Aspergillus spp. and Fusarium spp. were higher in trench silos, whereas Penicillium spp. was higher in silo bags. Silo bags showed less diversity than trench silos. Strains isolated produced AFB 1, FB 1 and PAT. In trench silos, AFB 1 was the only mycotoxin detected (1-160 μg kg -1). In silo bags AFB 1 levels varied from 5.8 to 47.4 μg kg -1. DON was detected in two silo bag samples. CONCLUSION: When handling is adequate the reduction of mould and mycotoxin contamination in silo bags is considerable. This study will enable estimation of the mycotoxicological risk of different ensiling practices and determination of the most adequate method to minimize economic losses and reduce hazard to animal and human health. © 2011 Society of Chemical Industry.

Registro:

Documento: Artículo
Título:Comparative analysis of the mycobiota and mycotoxins contaminating corn trench silos and silo bags
Autor:González Pereyra, M.L.; Chiacchiera, S.M.; Rosa, C.A.; Sager, R.; Dalcero, A.M.; Cavaglieri, L.
Filiación:Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Departamento de Química, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina
Departamento de Microbiologia e Imunología Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro 23890-000, Brazil
Conselho Nacional de Pesquisas Científicas (CNPq), Brasilia, Brazil
EEA San Luis, Instituto Nacional de Tecnología Agropecuaria (INTA), 5700 San Luis, Argentina
Idioma: Inglés
Palabras clave:Aflatoxins; Deoxynivalenol; Fumonisins; Mycobiota; Silage; mycotoxin; animal; article; biodiversity; cattle; cereal; comparative study; food contamination; fungus; isolation and purification; maize; metabolism; microbiology; silage; Animals; Biodiversity; Cattle; Cereals; Food Contamination; Fungi; Mycotoxins; Silage; Zea mays; Animalia; Aspergillus; Fusarium; Mycota; Penicillium; Zea mays
Año:2011
Volumen:91
Número:8
Página de inicio:1474
Página de fin:1481
DOI: http://dx.doi.org/10.1002/jsfa.4336
Título revista:Journal of the Science of Food and Agriculture
Título revista abreviado:J. Sci. Food Agric.
ISSN:00225142
CODEN:JSFAA
CAS:Mycotoxins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00225142_v91_n8_p1474_GonzalezPereyra

Referencias:

  • Sparo, M.D., Mallo, R.A., Evaluation of the bacterial flora in natural corn silage (2001) Rev Argent Microbiol, 33, pp. 75-80
  • Richard, E., Heutte, N., Bouchart, V., Garon, D., Evaluation of fungal contamination and mycotoxin production in maize silage (2009) Anim Feed Sci Technol, 148, pp. 309-320
  • Cleveland, T.E., Dowd, P.F., Desjardins, A.E., Bhatnagar, D., Cotty, P.Y., United States Department of Agriculture-Agricultural Research Service Research on pre-harvest prevention of mycotoxins and mycotoxigenic fungi in US crops (2003) Pest Manag Sci, 59, pp. 629-642
  • dos Santos, V.M., Dorner, J.W., Carreira, F., Isolation and toxigenicity of Aspergillus fumigatus from moldy silage (2002) Mycopathologia, 156, pp. 133-138
  • Frisvad, J.C., Thrane, U., Samson, R.A., Pitt, J.L., Important mycotoxins and the fungi which produce them (2006) Adv Exp Med Biol, 571, pp. 3-31
  • Kalac, P., Woolford, M.K., A review of some aspects of possible associations between the feeding of silage and animal health (1982) Br Vet J, 138, pp. 305-320
  • Bennett, J.W., Klich, M., Mycotoxins (2003) Clin Microbiol Rev, 16, pp. 497-516
  • Corbett, W.T., Brownie, C.F., Hagler, S.B., Hagler Jr, W.M., An epidemiological investigation associating aflatoxin M 1 with milk production in dairy cattle (1988) Vet Hum Toxicol, 30, pp. 5-8
  • (2003), CAST (Council for Agricultural Science and Technology), Mycotoxins: risks in plant, animal and human systems. Task Force Report No. 139, Ames, IAHoward, P.C., Eppley, R.M., Stack, M.E., Warbritton, A., Voss, K.A., Lorentzen, R.J., Fumonisin B 1 carcinogenicity in a two-year feeding study using F344 rats and B6C3F1 mice (2001) Environ Health Perspect, 109, pp. 277-282
  • Marasas, W.F.O., Discovery and occurrence of the fumonisins: a historical perspective (2001) Environ Health Perspect, 109, pp. 239-243
  • Smith, G.W., Constable, P.D., Foreman, J.H., Eppley, R.M., Waggoner, A.L., Tumbleson, M.E., Cardiovascular changes associated with intravenous administration of fumonisin B 1 in horses (2002) Am J Vet Res, 63, pp. 538-545
  • Rice, L.G., Ross, P.F., Methods for detection and quantitation of fumonisins in corn, cereal products and animal excreta (1994) J Food Prot, 57, pp. 536-540
  • Black, R.D., McVey, D.S., Oehme, F.W., Immunotoxicity in the bovine animal: a review (1992) Vet Hum Toxicol, 34, pp. 438-442
  • Tapia, M.O., Stern, M.D., Soraci, A.L., Meronuckc, R., Olson, W., Patulin-producing molds in corn silage and high moisture corn and effects of patulin on fermentation by ruminal microbes in continuous culture (2005) Anim Feed Sci Technol, 119, pp. 247-258
  • Hori, M., Yamamoto, T., Ozawa, A., Matzuki, Y., Hamaguchi, A., Soraoka, H., Studies on a fungus species isolated from a malt feed which caused mass death of cows (1954) Jpn J Bacteriol, 9, pp. 1105-1111
  • Moreau, C., Moreau, M., Un danger pour le bétail nourri de plantules fourragères cultivées en germoirs: lapullulation d'une moisissure toxique, l'Aspergillus clavatus, cause des accidents mortels (1960) CR Seances Acad Agric Fr, 46, pp. 441-445
  • Jaquet, J., Boutibonnes, P., Cicile, J.P., Observations sur la noticite d'Aspergillus clavatus pour les animaux (1963) Bull Acad Vet, 32, pp. 119-207
  • Schultz, V.J., Mortz, R., Schafer, M., Aumgart, W., Experimentelle untersuchungenzur Aspergillus clavatus vergiftung beim rind (1968) Monatschr. Veterinamed., 24, pp. 14-17
  • Syret, R., (1977), pp. 4-7. , Moulds and mycotoxins in animal foodstuffs. Reports of ADAS Microbiologists -Scudamore, K.A., Livesey, C.T., Occurrence and significance of mycotoxins in forage crops and silage: a review (1998) J Sci Food Agric, 77, pp. 1-7
  • Ohyama, Y., Masaki, S., Hara, S., Factors influencing aerobic deterioration of silages and changes in chemical composition after opening silos (1975) J Sci Food Agric, 26, pp. 1137-1147
  • Abarca, M.L., Bragulat, M.R., Castella, G., Cabañes, F.J., Mycoflora and aflatoxin-producing strains in animal mixed feeds (1994) J Food Prot, 57, pp. 256-258
  • (1997) Fungi and Food Spoilage, , Pitt JI and Hocking AD (eds), 2nd edn). Blackie Academic Press, London
  • Nelson, P.E., Toussoun, T.A., Marasas, W.F.O., (1983) Fusarium Species: An Illustrated Manual for Identification, , Pennsylvania State University Press, University Park
  • Samson, R.A., Hockstra, E.S., Frisvad, J.C., Filtenborg, O., Introduction to Food and Airborne Fungi (2000) Centaalbureau Voorschimmelculturs-Utrecht Ponson & Looyen, , Wageningen Press, Wageningen
  • Klich, M.A., (2002) Identification of Common Aspergillus Species, , CBS, Utrecht
  • Frisvad, J.C., Samson, R.A., Polyphasic taxonomy of Penicillium subgenus Penicillium: a guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins (2004) Stud Mycol, 49, pp. 1-174
  • Geisen, R., Multiplex polymerase chain reaction for the detection of potential aflatoxin and sterigmatocystin producing fungi (1996) System Appl Microbiol, 19, pp. 388-392
  • Shephard, G.S., Sydenham, E.W., Thiel, P.G., Gelderblom, W.C.A., Quantitative determination of fumonisin B 1 and B 2 by high-performance liquid chromatography with fluorescence detection (1990) J Liquid Chromatogr, 13, pp. 2077-2087
  • Doko, B., Rapior, S., Visconti, A., Schjoth, J., Incidence and levels of fumonisin contamination in maize genotypes grown in Europe and Africa (1995) J Agric Food Chem, 43, pp. 429-434
  • Trucksess, M.W., Stack, M.E., Nesheim, S., Albert, R.H., Romer, T.R., Multifunctional column coupled with liquid chromatography for determination of aflatoxins B 1, B 2, G 1 and G 2 in corn, almonds, Brazil nuts, peanuts and pistachio nuts: collaborative study (1994) J AOAC Int, 77, pp. 1512-1521
  • (1995) Official Methods of Analysis, , AOAC International, Vol. 1 (16th edn). AOAC International, Arlington, VA
  • (2002) Experimental Design Data Analysis for Biologists, , Quinn GP and Keough MJ (eds), Cambridge University Press, Cambridge, UK
  • GMP, Certification Scheme Animal Feed Sector 2006, Including Residue Standards (Version 28, March 2008)El-Shanawany, A.A., Eman Mostafa, M., Barakat, A., Fungal populations and mycotoxins in silage in Assiut and Sohag governorates in Egypt, with a special reference to characteristic Aspergilli toxins (2005) Mycopathologia, 159, pp. 281-289
  • Dalcero, A., Magnoli, C., Chiacchiera, S., Palacios, G., Reynoso, M., Mycoflora and incidence of aflatoxin B 1, zearalenone and deoxynivalenol in poultry feeds in Argentine (1997) Mycopathologia, 137, pp. 179-184
  • Dalcero, A., Magnoli, C., Luna, M., Ancasi, G., Reynoso, M.M., Chiachiera, S., Mycoflora and naturally occurring mycotoxins in poultry feeds in Argentina (1998) Mycopathologia, 141, pp. 37-43
  • Magnoli, C., Chiacchiera, S., Miazzo, R., Palacio, G., Angeletti, A., Hallak, C., The mycoflora and toxicity of feedstuffs from a production plant in Córdoba, Argentina (2002) Mycotoxicol Res, 18, pp. 7-22
  • Rosa, C.A.R., Ribeiro, J.M.M., Fraga, M.J., Gatti, M.J., Cavaglieri, L.R., Magnoli, C.E., Mycoflora of poultry feeds and ochratoxin-producing ability of isolated Aspergillus and Penicillium species (2006) Vet Microbiol, 113, pp. 89-96
  • Keller, K.M., Keller, L.A.M., Queiroz, B.D., Oliveira, A., Almeida, T.X., Marassi, A.C., Study on the mycobiota and mycotoxins of commercial equine feeds in Rio de Janeiro, Brazil (2008) Ver Bras Med Vet, 30, pp. 224-229
  • Keller, K.M., Queiroz, B.D., Keller, L.A.M., Ribeiro, J.M.M., Cavaglieri, L.R., González, P.M.L., The mycobiota and toxicity of equine feeds, (2007) Vet Res Commun, 31, pp. 1037-1045
  • González Pereyra, M.L., Alonso, V.A., Sager, R., Morlaco, M.B., Magnoli, C.E., Astoreca, A.L., Fungi and selected mycotoxins from pre- and post- fermented corn silage (2008) J Appl Microbiol, 104, pp. 1034-1041
  • González Pereyra, M.L., Keller, K.M., Keller, L.A.M., Cavaglieri, L.R., Queiroz, B., Tissera, J., Mycobiota and mycotoxins of equine feedstuffs in the central region of Argentina (2009) Rev Bras Med Vet, 31, pp. 24-29
  • González Pereyra, M.L., Pereyra, C.M., Ramirez, M.L., Rosa, C.A.R., Dalcero, A.M., Cavaglieri, L.R., Determination of mycobiota and mycotoxins in pig feed in central Argentina (2008) Lett Appl Microbiol, 46, pp. 555-561
  • Richard, E., Heutte, N., Sage, L., Pottier, D., Bouchart, V., Lebailly, P., Toxigenic fungi and mycotoxins in mature corn silage (2007) Food Chem Toxicol, 45, pp. 2420-2425
  • Garon, D., Richard, E., Sage, L., Bouchart, V., Pottier, D., Lebailly, P., Mycoflora and multimycotoxin detection in corn silage: experimental study (2006) J Agric Food Chem, 54, pp. 3479-3484
  • Pereyra, C.M., Alonso, V.A., Rosa, C.A.R., Chiacchiera, S.M., Dalcero, A.M., Cavaglieri, L.R., Gliotoxin natural incidence of Aspergillus fumigatus isolated from corn silage and ready dairy cattle feed (2008) World Mycotoxin J, 1, pp. 457-462
  • O'Brien, M., O'Kiely, P., Forristal, P.D., Fuller, H.T., Fungi isolated from contaminated baled grass silage on farms in the Irish Midlands (2005) FEMS Microbiol Lett, 247, pp. 131-135
  • European Union, Commission Recommendation of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding (2006/576/EC). [Online]. Official J EU. Available: [4 February 2010]@@@!v7020!http://www.scopus.com/inward/record.url?eid=2-s2.0-79955523405&partnerID=40&md5=9c83317f629b317af9568a5fac3cc16d

Citas:

---------- APA ----------
González Pereyra, M.L., Chiacchiera, S.M., Rosa, C.A., Sager, R., Dalcero, A.M. & Cavaglieri, L. (2011) . Comparative analysis of the mycobiota and mycotoxins contaminating corn trench silos and silo bags. Journal of the Science of Food and Agriculture, 91(8), 1474-1481.
http://dx.doi.org/10.1002/jsfa.4336
---------- CHICAGO ----------
González Pereyra, M.L., Chiacchiera, S.M., Rosa, C.A., Sager, R., Dalcero, A.M., Cavaglieri, L. "Comparative analysis of the mycobiota and mycotoxins contaminating corn trench silos and silo bags" . Journal of the Science of Food and Agriculture 91, no. 8 (2011) : 1474-1481.
http://dx.doi.org/10.1002/jsfa.4336
---------- MLA ----------
González Pereyra, M.L., Chiacchiera, S.M., Rosa, C.A., Sager, R., Dalcero, A.M., Cavaglieri, L. "Comparative analysis of the mycobiota and mycotoxins contaminating corn trench silos and silo bags" . Journal of the Science of Food and Agriculture, vol. 91, no. 8, 2011, pp. 1474-1481.
http://dx.doi.org/10.1002/jsfa.4336
---------- VANCOUVER ----------
González Pereyra, M.L., Chiacchiera, S.M., Rosa, C.A., Sager, R., Dalcero, A.M., Cavaglieri, L. Comparative analysis of the mycobiota and mycotoxins contaminating corn trench silos and silo bags. J. Sci. Food Agric. 2011;91(8):1474-1481.
http://dx.doi.org/10.1002/jsfa.4336