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Abstract:

Micellar electrokinetic capillary chromatography (MECC) experimental conditions were applied to 12 samples of methylxanthine-containing infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. The best resolution in this mode of automated high-performance capillary electrophoresis (HPCE) was achieved here when using 15 kV voltage in an uncoated fused-silica capillary of 45 cm length (40 cm effective length), 50 mM sodium dodecylsulfate, 90 mM pH 8.5 borate buffer and UV detection. Theobromine, caffeine and theophylline were separated, and the peak splitting due to tautomeric species was observed. Experimental conditions were controlled, keeping constant the size of the elution window in each analysis. The limit of detection was less than 1 mgl -1, the limit of quantitation was 2.5 mgl -1 and the work range was 2.5-300 mgl -1. This HPCE-MECC system has proved suitable for the analysis/quality control of xanthines in beverages for consumption. Roles of various parameters as well as distinctly charged species of each xanthine and the origin of peak splitting in this MECC system are discussed. © 2004 Society of Chemical Industry.

Registro:

Documento: Artículo
Título:Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved
Autor:Pomilio, A.B.; Trajtemberg, S.P.; Vitale, A.A.
Filiación:PROPLAME-CONlCET, Depto. de Quim. Orgánica, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
PROPLAME-CONICET, Depto. de Quim. Orgánica, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Natl. Research Council of Argintina, Argentina
Palabras clave:Automated high-performance capillary electrophoresis (HPCE); Beverages; Caffeine; Keto-enol tautomerism; Micellar electrokinetic capillary chromatography (MECC); Theobromine; Theophylline; Cola; Croton ovalifolius; Ilex paraguariensis; Theobroma cacao
Año:2005
Volumen:85
Número:4
Página de inicio:622
Página de fin:628
DOI: http://dx.doi.org/10.1002/jsfa.2020
Título revista:Journal of the Science of Food and Agriculture
Título revista abreviado:J. Sci. Food Agric.
ISSN:00225142
CODEN:JSFAA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00225142_v85_n4_p622_Pomilio

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Citas:

---------- APA ----------
Pomilio, A.B., Trajtemberg, S.P. & Vitale, A.A. (2005) . Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved. Journal of the Science of Food and Agriculture, 85(4), 622-628.
http://dx.doi.org/10.1002/jsfa.2020
---------- CHICAGO ----------
Pomilio, A.B., Trajtemberg, S.P., Vitale, A.A. "Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved" . Journal of the Science of Food and Agriculture 85, no. 4 (2005) : 622-628.
http://dx.doi.org/10.1002/jsfa.2020
---------- MLA ----------
Pomilio, A.B., Trajtemberg, S.P., Vitale, A.A. "Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved" . Journal of the Science of Food and Agriculture, vol. 85, no. 4, 2005, pp. 622-628.
http://dx.doi.org/10.1002/jsfa.2020
---------- VANCOUVER ----------
Pomilio, A.B., Trajtemberg, S.P., Vitale, A.A. Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved. J. Sci. Food Agric. 2005;85(4):622-628.
http://dx.doi.org/10.1002/jsfa.2020