Abstract:
Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress were studied and related to tissue textural behavior. Turgor pressure of raw tissue was adjusted by immersion in hypotonic (0.0 mol m-3), isotonic (250 mol m-3) and plasmolyzing (1050 mol m-3) buffered (20 mol m-3 potassium phosphate, pH 6.8) solutions of poly(ethylene glycol) 400 (PEG). It was found that a substantial proportion of polysaccharides located in the cell wall (CW)-middle lamellae (ML) of studied tissue was water extractable. This proportion decreased when CW-stretch increased. Ionically bound peroxidase (POX) presented an increased activity in swollen and isotonic tissue, probably associated to oxidative cross-linking of extensin located in the CW of this tissue, as a response to stress promoted by cut, immersion and turgor pressure change. The resistance of CW to elongation when equilibrated in hypotonic medium, might be ascribed to reinforcement of the CW by the oxidative cross-linking of extensin, with simultaneous formation of pectin-in-extensin entanglements. Plasmolyzed tissue where CW is relaxed, showed pectin-rhamnogalacturonan degradation. The water-soluble fraction (WSF) was shown to be a negative function of CW stretching and infinite force of relaxation. © 2005 Society of Chemical Industry.
Registro:
Documento: |
Artículo
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Título: | Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress |
Autor: | De Escalada Pla, M.F.; Ponce, N.M.; Wider, M.E.; Stortz, C.A.; Rojas, A.M.; Gerschenson, L.N. |
Filiación: | Departamento de Industrias, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (1428) Buenos Aires, Argentina Departamento de Química Orgánica - CIHIDECAR, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (1428) Buenos Aires, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (1428) Buenos Aires, Argentina National Research Council, CONICET, Argentina
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Palabras clave: | Enzymes; Pectins; Polysaccharides; Pumpkin; Turgor pressure; Cucumis |
Año: | 2005
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Volumen: | 85
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Número: | 11
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Página de inicio: | 1852
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Página de fin: | 1860
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DOI: |
http://dx.doi.org/10.1002/jsfa.2187 |
Título revista: | Journal of the Science of Food and Agriculture
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Título revista abreviado: | J. Sci. Food Agric.
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ISSN: | 00225142
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CODEN: | JSFAA
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00225142_v85_n11_p1852_DeEscaladaPla |
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Citas:
---------- APA ----------
De Escalada Pla, M.F., Ponce, N.M., Wider, M.E., Stortz, C.A., Rojas, A.M. & Gerschenson, L.N.
(2005)
. Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress. Journal of the Science of Food and Agriculture, 85(11), 1852-1860.
http://dx.doi.org/10.1002/jsfa.2187---------- CHICAGO ----------
De Escalada Pla, M.F., Ponce, N.M., Wider, M.E., Stortz, C.A., Rojas, A.M., Gerschenson, L.N.
"Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress"
. Journal of the Science of Food and Agriculture 85, no. 11
(2005) : 1852-1860.
http://dx.doi.org/10.1002/jsfa.2187---------- MLA ----------
De Escalada Pla, M.F., Ponce, N.M., Wider, M.E., Stortz, C.A., Rojas, A.M., Gerschenson, L.N.
"Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress"
. Journal of the Science of Food and Agriculture, vol. 85, no. 11, 2005, pp. 1852-1860.
http://dx.doi.org/10.1002/jsfa.2187---------- VANCOUVER ----------
De Escalada Pla, M.F., Ponce, N.M., Wider, M.E., Stortz, C.A., Rojas, A.M., Gerschenson, L.N. Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress. J. Sci. Food Agric. 2005;85(11):1852-1860.
http://dx.doi.org/10.1002/jsfa.2187