Artículo

De Escalada Pla, M.F.; Ponce, N.M.; Wider, M.E.; Stortz, C.A.; Rojas, A.M.; Gerschenson, L.N. "Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress" (2005) Journal of the Science of Food and Agriculture. 85(11):1852-1860
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Abstract:

Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress were studied and related to tissue textural behavior. Turgor pressure of raw tissue was adjusted by immersion in hypotonic (0.0 mol m-3), isotonic (250 mol m-3) and plasmolyzing (1050 mol m-3) buffered (20 mol m-3 potassium phosphate, pH 6.8) solutions of poly(ethylene glycol) 400 (PEG). It was found that a substantial proportion of polysaccharides located in the cell wall (CW)-middle lamellae (ML) of studied tissue was water extractable. This proportion decreased when CW-stretch increased. Ionically bound peroxidase (POX) presented an increased activity in swollen and isotonic tissue, probably associated to oxidative cross-linking of extensin located in the CW of this tissue, as a response to stress promoted by cut, immersion and turgor pressure change. The resistance of CW to elongation when equilibrated in hypotonic medium, might be ascribed to reinforcement of the CW by the oxidative cross-linking of extensin, with simultaneous formation of pectin-in-extensin entanglements. Plasmolyzed tissue where CW is relaxed, showed pectin-rhamnogalacturonan degradation. The water-soluble fraction (WSF) was shown to be a negative function of CW stretching and infinite force of relaxation. © 2005 Society of Chemical Industry.

Registro:

Documento: Artículo
Título:Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress
Autor:De Escalada Pla, M.F.; Ponce, N.M.; Wider, M.E.; Stortz, C.A.; Rojas, A.M.; Gerschenson, L.N.
Filiación:Departamento de Industrias, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (1428) Buenos Aires, Argentina
Departamento de Química Orgánica - CIHIDECAR, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (1428) Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (1428) Buenos Aires, Argentina
National Research Council, CONICET, Argentina
Palabras clave:Enzymes; Pectins; Polysaccharides; Pumpkin; Turgor pressure; Cucumis
Año:2005
Volumen:85
Número:11
Página de inicio:1852
Página de fin:1860
DOI: http://dx.doi.org/10.1002/jsfa.2187
Título revista:Journal of the Science of Food and Agriculture
Título revista abreviado:J. Sci. Food Agric.
ISSN:00225142
CODEN:JSFAA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00225142_v85_n11_p1852_DeEscaladaPla

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Citas:

---------- APA ----------
De Escalada Pla, M.F., Ponce, N.M., Wider, M.E., Stortz, C.A., Rojas, A.M. & Gerschenson, L.N. (2005) . Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress. Journal of the Science of Food and Agriculture, 85(11), 1852-1860.
http://dx.doi.org/10.1002/jsfa.2187
---------- CHICAGO ----------
De Escalada Pla, M.F., Ponce, N.M., Wider, M.E., Stortz, C.A., Rojas, A.M., Gerschenson, L.N. "Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress" . Journal of the Science of Food and Agriculture 85, no. 11 (2005) : 1852-1860.
http://dx.doi.org/10.1002/jsfa.2187
---------- MLA ----------
De Escalada Pla, M.F., Ponce, N.M., Wider, M.E., Stortz, C.A., Rojas, A.M., Gerschenson, L.N. "Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress" . Journal of the Science of Food and Agriculture, vol. 85, no. 11, 2005, pp. 1852-1860.
http://dx.doi.org/10.1002/jsfa.2187
---------- VANCOUVER ----------
De Escalada Pla, M.F., Ponce, N.M., Wider, M.E., Stortz, C.A., Rojas, A.M., Gerschenson, L.N. Chemical and biochemical changes of pumpkin (Cucumis moschata, Duch) tissue in relation to osmotic stress. J. Sci. Food Agric. 2005;85(11):1852-1860.
http://dx.doi.org/10.1002/jsfa.2187