Artículo

Keller, L.A.M.; González Pereyra, M.L.; Keller, K.M.; Alonso, V.A.; Oliveira, A.A.; Almeida, T.X.; Barbosa, T.S.; Nunes, L.M.T.; Cavaglieri, L.R.; Rosa, C.A.R. "Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation" (2013) Journal of Stored Products Research. 52:42-47
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Silage is a widespread practice to preserve forage. Poor storage conditions can lead to mold contamination and mycotoxin production. The aim of this study was to establish the occurrence of toxigenic fungal species and to determine aflatoxins (AFs), ochratoxin A (OTA), fumonisin B1 (FB1) and deoxinivalenol (DON) in corn silage intended for bovines before and after fermentation in farms located in São Paulo and Rio de Janeiro States, Brazil. Fungal counts were done by surface-spread method. Toxigenic ability of isolates was evaluated in vitro. AFs natural contamination was determined by TLC and HPLC. Total fungal counts were generally high. Aspergillus flavus, Penicillium citrinum, and Fusarium verticillioides were the prevalent species. Toxigenic strains were isolated. Aflatoxin levels differed (P < 0.0001) from 2 to 45 μg g-1 and from 2 to 100 μg g-1 in pre and post-fermentation samples, respectively. Ochratoxin A, FB1 and DON levels found in pre-fermentation samples were higher than in post-fermentation (P < 0.0001). Mycotoxins and toxigenic fungi were present before and after fermentation in corn silage intended for bovines in Brazil. Aflatoxin B1 (AFB1) increased during storage whereas OTA, FB1 and DON decreased. Determination of mycotoxin levels and their occurrence in corn silage are important to provide information so that the assessments of risk for animal feed and livestock environment could be made. © 2012 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation
Autor:Keller, L.A.M.; González Pereyra, M.L.; Keller, K.M.; Alonso, V.A.; Oliveira, A.A.; Almeida, T.X.; Barbosa, T.S.; Nunes, L.M.T.; Cavaglieri, L.R.; Rosa, C.A.R.
Filiación:Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rodovia BR 465 Km. 7, Seropédica, Rio de Janeiro 23890-000, Brazil
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Av. Antonio Carlos, 6627 Belo Horizonte, MG 31270-901, Brazil
Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km. 601, 5800 Río Cuarto, Córdoba, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAV Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917, C1033AAV Buenos Aires, Argentina
Conselho Nacional de Pesquisas Científicas (CNPq), Av. Antonio Carlos, 6627 Belo Horizonte, MG 31270-901, Brazil
Idioma: Inglés
Palabras clave:Aflatoxin B1; Corn silage; Deoxinivalenol; Fumonisin B1; Fungi; Ochratoxin A; Animalia; Aspergillus flavus; Fungi; Gibberella fujikuroi; Gibberella moniliformis; Penicillium citrinum; Zea mays
Año:2013
Volumen:52
Página de inicio:42
Página de fin:47
DOI: http://dx.doi.org/10.1016/j.jspr.2012.09.001
Título revista:Journal of Stored Products Research
Título revista abreviado:J. Stored Prod. Res.
ISSN:0022474X
CODEN:JSTPA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022474X_v52_n_p42_Keller

Referencias:

  • (2000) Association of Official Analytical Chemists (AOAC) Official Methods of Analysis. AOAC International, , Maryland, USA, Gaithersburg
  • Bennett, J.W., Klich, M., Mycotoxins (2003) Clinical Microbiology Reviews, 16, pp. 497-516
  • Black, R.D., McVey, D.S., Oehme, F.W., Immunotoxicity in the bovine animal: a review (1992) Veterinary and Human Toxicology, 34 (5), pp. 438-442
  • Bodine, A.B., Mertens, D.R., Toxicology, metabolism and physiological effects of aflatoxin in the bovine (1983) Aflatoxin and Aspergillus flavus in Corn, pp. 46-50. , Auburn University. Alabama Ag. Exp. Sta., Alabama, USA, U.L. Diener, R.L. Asquith, J.W. Dickens (Eds.)
  • Boysen, M.E., Jacobson, K.G., Schnurer, J., Molecular identification of species from the Penicillium roqueforti group associated with spoiled animal feed (2000) Applied and Environmental Microbiology, 66, pp. 1523-1526
  • (2003) Council for Agricultural Science and Technology (CAST), , Mycotoxins: Risks in Plant, Animal and Human Systems, Task Force Report N°139, Ames, Iowa, USA
  • Cleveland, T.E., Dowd, P.F., Desjardins, A.E., Bhatnagar, D., Cotty, P.Y., United States Department of Agriculture-Agricultural Research Service research on pre-harvest prevention of mycotoxins and mycotoxigenic fungi in US crops (2003) Pest Management Science, 59 (5-6), pp. 629-642
  • Corbett, W.T., Brownie, C.F., Hagler, S.B., Hagler, W.M., An epidemiological investigation associating aflatoxin M1 with milk production in dairy cattle (1988) Veterinary and Human Toxicology, 30 (1), pp. 5-8
  • Dos Santos, V.M., Dorner, J.W., Carreira, F., Isolation and toxigenicity of Aspergillus fumigatus from moldy silage (2003) Mycopathologia, 156 (2), pp. 133-138
  • El-Shanawany, A.A., Eman Mostafa, M., Barakat, A., Fungal populations and mycotoxins in silage in Assiut and Sohag governorates in Egypt, with a special reference to characteristic Aspergilli toxins (2005) Mycopathologia, 159, pp. 281-289
  • European Union (EU) Commission recommendation of 17 august 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding (2006/576/EC) (2006) Official Journal of European Union, , http://eurlex.europa.eu/LexUriServ/LexUriServ.do%3Furi%3DOJ:L:2006:229:0007:0009:EN:PDF, Available on: URL:, (accessed on 17.08.06.)
  • Frisvad, J.C., Thrane, U., Samson, R.A., Pitt, J.L., Important mycotoxins and the fungi which produce them (2006) Advances in Experimental Medicine and Biology, 571, pp. 3-31
  • Geisen, R., Multiplex polymerase chain reaction for the detection of potential aflatoxin and sterigmatocystin producing fungi (1996) Systematic and Applied Microbiology, 19 (3), pp. 388-392
  • (2008) GMP Certification Scheme Animal Feed Sector 2006, Including Residue Standards, , Version 28
  • González Pereyra, M.L., Alonso, V.A., Sager, R., Morlaco, M.B., Magnoli, C.E., Astoreca, A.L., Rosa, C.A.R., Cavaglieri, L.R., Fungi and selected mycotoxins from pre- and post- fermented corn silage (2008) Journal of Applied Microbiology, 104 (4), pp. 1034-1041
  • Howard, P.C., Eppley, R.M., Stack, M.E., Warbritton, A., Voss, K.A., Lorentzen, R.J., Kovach, R.M., Bucci, T.J., Fumonisin B1 carcinogenicity in a two-year feeding study using F344 rats and B6C3F1 mice (2001) Environmental Health Perspectives, 109 (2), pp. 277-282
  • Kalac, P., Woolford, M.K., A review of some aspects of possible associations between the feeding of silage and animal health (1982) The British Veterinary Journal, 138, pp. 305-320
  • Kim, E.K., Maragos, C.H., Kendra, D.F., Liquid chromatography determination of fumonisins B1, B2 and B3 in corn silage (2004) Journal of Agricultural and Food Chemistry, 52, pp. 196-200
  • Klich, M.A., (2002) Identification of Common Aspergillus Species, , CBS, Utrecht, Netherlands
  • Marasas, W.F.O., Discovery and occurrence of the fumonisins: a historical perspective (2001) Environmental Health Perspectives, 109 (2), pp. 239-243
  • Marasas, W.F.O., Miller, J.D., Riley, R.T., Visconti, A., (2000) Environmental Health Criteria 219. Fumonisin B1, , International Programme on Chemical Safety, World Health Organization, Geneva, Switzerland
  • Moreno, E.C., García, G.T., Ono, M.A., Vizoni, E., Kawamura, O., Hirooka, E.Y., Ono, E.Y.S., Co-occurrence of mycotoxins in corn samples from the northern region of Paraná state, Brazil (2009) Food Chemistry, 116 (1), pp. 220-226
  • Nelson, P.E., Toussoun, T.A., Marasas, W.F.O., (1983) Fusarium Species: An Illustrated Manual for Identification, , The Pennsylvania State University Press, University Park and London, UK
  • Nout, M.J.R., Bouwmeester, H.M., Haaskma, J., Van Dijk, H., Fungal growth in silages of sugarbeet press pulp and corn (1993) The Journal of Agricultural Science, 121, pp. 323-326
  • Ohyama, Y., Masaki, S., Hara, S., Factors influencing aerobic deterioration of silages and changes in chemical composition after opening silos (1975) Journal of the Science of Food and Agriculture, 26, pp. 1137-1147
  • Osweiler, G.D., Kehrli, M.E., Stabel, J.R., Thurston, J.R., Ross, P.F., Wilson, T.M., Effects of fumonisin-contaminated corn screenings on growth and health of feeder calves (1993) Journal of Animal Science, 71, pp. 459-466
  • (1997) Fungi and Food Spoilage, , Blackie Academic Press, London, J.I. Pitt, A.D. Hocking (Eds.)
  • (2002) Experimental Design Data Analysis for Biologists, , Cambridge University Press, Cambridge, UK, G.P. Quinn, M.J. Keough (Eds.)
  • Reyes-Velázquez, W.P., Espinoza, V.H.I., Rojo, F., Jiménez-Plasencia, C., Palacios, E.L., Hernández-Góbora, J., Ramírez-álvarez, A., Occurrence of fungi and mycotoxins in corn silage, Jalisco state, Mexico (2008) Revista Iberoamericana de Micología, 25, pp. 182-185
  • Rice, L.G., Ross, P.F., Methods for detection and quantitation of fumonisins in corn, cereal products and animal excreta (1994) Journal of Food Protection, 57, pp. 536-540
  • Richard, E., Heutte, N., Bouchart, V., Garon, D., Evaluation of fungal contamination and mycotoxin production in maize silage (2009) Animal Feed Science and Technology, 148, pp. 309-320
  • Richard, E., Heutte, N., Sage, L., Pottier, D., Bouchart, V., Lebailly, P., Garon, D., Toxigenic fungi and mycotoxins in mature corn silage (2007) Food Chemical Toxicology, 45, pp. 2420-2425
  • Rodrigues, P.B., Freitas, R.T.F., Fialho, E.T., Silva, H.O., Tascisio Morais Gonçalves, T.M., Digestibilidade dos nutrientes e desempenho de suínos em crescimento e terminação alimentados com rações à base de milho e sorgo suplementadas com enzimas (2002) Revista Brasileira de Milho e Sorgo, 1 (2), pp. 91-100
  • Samson, R.A., Frisvad, J.C., Polyphasic taxonomy of Penicillium subgenus Penicillium. A guide to identification of food and air-borne terverticillat Penicillia and their mycotoxins (2004) Studies in Mycology, 49, pp. 1-174
  • Samson, R.A., Hockstra, E.S., Frisvad, J.C., Filtenborg, O., (2000) Introduction to Food and Airborne Fungi, , Centaalbureau Voorschimmelculturs-Utrecht Ponson Looyen, Wageningen Press, The Netherlands
  • Scudamore, K.A., Nawaz, S., Hetmanski, M.T., Mycotoxins in ingredients of animal feeding stuffs: II. Determination of mycotoxins in maize and maize products (1998) Food Additives and Contaminants, 15, pp. 30-55
  • Smith, G.W., Constable, P.D., Foreman, J.H., Eppley, R.M., Waggoner, A.L., Tumbleson, M.E., Haschek, W.M., Cardiovascular changes associated with intravenous administration of fumonisin B1 in horses (2002) American Journal of Veterinary Research, 63, pp. 538-545
  • Soares, L.M.V., Rodeigues-Amaya, D., Survey of aflatoxins, ochratoxin A, zearalenone, and sterigmatocystin in some Brazilian foods by using multi-toxin thin-layer chromatographic method (1989) Journal - Association of Official Analytical Chemists, 72 (1), pp. 22-26
  • Téren, J., Varga, J., Hamari, Z., Rinyu, E., Kevei, F., Immunochemical detection of ochratoxin A in black Aspergillus strains (1996) Mycopathologia, 134 (3), pp. 171-176

Citas:

---------- APA ----------
Keller, L.A.M., González Pereyra, M.L., Keller, K.M., Alonso, V.A., Oliveira, A.A., Almeida, T.X., Barbosa, T.S.,..., Rosa, C.A.R. (2013) . Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation. Journal of Stored Products Research, 52, 42-47.
http://dx.doi.org/10.1016/j.jspr.2012.09.001
---------- CHICAGO ----------
Keller, L.A.M., González Pereyra, M.L., Keller, K.M., Alonso, V.A., Oliveira, A.A., Almeida, T.X., et al. "Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation" . Journal of Stored Products Research 52 (2013) : 42-47.
http://dx.doi.org/10.1016/j.jspr.2012.09.001
---------- MLA ----------
Keller, L.A.M., González Pereyra, M.L., Keller, K.M., Alonso, V.A., Oliveira, A.A., Almeida, T.X., et al. "Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation" . Journal of Stored Products Research, vol. 52, 2013, pp. 42-47.
http://dx.doi.org/10.1016/j.jspr.2012.09.001
---------- VANCOUVER ----------
Keller, L.A.M., González Pereyra, M.L., Keller, K.M., Alonso, V.A., Oliveira, A.A., Almeida, T.X., et al. Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation. J. Stored Prod. Res. 2013;52:42-47.
http://dx.doi.org/10.1016/j.jspr.2012.09.001