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Abstract:

Metabolic properties and subcellular localization of the biosynthesis of SM, a saponifiable 18-OH-B (18-Hydroxycorticosterone) derivative, were investigated. Homogenates biosynthesized SM at a nearly constant rate of 463 pmol/50 mg tissue during 30 min. This biosynthesis was more efficient at pH 7.4 than at pH 4.8. Not only 18-OH-B but also its less polar anhydride 18-DAL (18-Deoxyaldosterone) were good precursors. SM was reverted to these precursors both enzymatically and spontaneously, 4.8 being a more suitable pH for this reversion than 7.4. Trapping experiments demonstrated a sequence comprising, in this order, the following echelons: SM, 18-OH-B, 18-DAL, Aldosterone. The first two steps are reversible and the last two ones depend on proton concentrations. It is postulated that SM could be on a dead-end to which 18-OH-B could be deviated if Aldosterone biosynthesis became temporarily unnecessary. Also, that 18-OH-B may convert to either 18-DAL or SM for selective membrane transports, according to homeostatic requirements. © 1987.

Registro:

Documento: Artículo
Título:Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
Autor:Cozza, E.N.; Ceballos, N.R.; del Carmen Vila, M.; Lantos, C.P.
Filiación:PRHOM (CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Buenos Aires (1428), Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Buenos Aires (1428), Argentina
Palabras clave:18 hydroxycorticosterone; corticosterone; drug derivative; adrenal gland; animal; article; cell fractionation; cell nucleus; cytosol; kinetics; metabolism; microsome; mitochondrion; rat; rat strain; solubility; 18-Hydroxycorticosterone; Adrenal Glands; Animal; Cell Nucleus; Corticosterone; Cytosol; Kinetics; Microsomes; Mitochondria; Rats; Rats, Inbred Strains; Solubility; Subcellular Fractions; Support, Non-U.S. Gov't
Año:1987
Volumen:28
Número:5
Página de inicio:543
Página de fin:547
DOI: http://dx.doi.org/10.1016/0022-4731(87)90513-9
Título revista:Journal of Steroid Biochemistry
Título revista abreviado:J. Steroid Biochem.
ISSN:00224731
CODEN:JSTBB
CAS:18 hydroxycorticosterone, 561-65-9; corticosterone, 50-22-6; 18-Hydroxycorticosterone, 561-65-9; Corticosterone, 50-22-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v28_n5_p543_Cozza

Referencias:

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Citas:

---------- APA ----------
Cozza, E.N., Ceballos, N.R., del Carmen Vila, M. & Lantos, C.P. (1987) . Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone. Journal of Steroid Biochemistry, 28(5), 543-547.
http://dx.doi.org/10.1016/0022-4731(87)90513-9
---------- CHICAGO ----------
Cozza, E.N., Ceballos, N.R., del Carmen Vila, M., Lantos, C.P. "Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone" . Journal of Steroid Biochemistry 28, no. 5 (1987) : 543-547.
http://dx.doi.org/10.1016/0022-4731(87)90513-9
---------- MLA ----------
Cozza, E.N., Ceballos, N.R., del Carmen Vila, M., Lantos, C.P. "Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone" . Journal of Steroid Biochemistry, vol. 28, no. 5, 1987, pp. 543-547.
http://dx.doi.org/10.1016/0022-4731(87)90513-9
---------- VANCOUVER ----------
Cozza, E.N., Ceballos, N.R., del Carmen Vila, M., Lantos, C.P. Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone. J. Steroid Biochem. 1987;28(5):543-547.
http://dx.doi.org/10.1016/0022-4731(87)90513-9