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Vazquez, M.H.; de Larminat, M.-A.; Gurpide, E.; Scorticati, C.; Blaquier, J.A. "Androgen metabolism in the human epididymis. effect of in vivo estrogen administration" (1986) Journal of Steroid Biochemistry. 25(2):239-244
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Abstract:

Androgen metabolism in human epididymis was studied by incubating tissue fragments with isotopically labeled testosterone (T) and androstenedione (A) under batch and superfusion conditions. Epididymides were obtained from 16 patients with prostatic cancer, 5 of them treated with diethyl-stilbestrol (2.5 mg/d) for several months prior to castration. Results from batch incubations with [3H]T (100nM) for 2 h at 25°C indicated a markedly lower 5α-reductase activity in tissues from estrogen-treated patients, as evaluated by measuring the amounts of radioactive 5α-dihydrotestosterone, 5α-androstanediols and 5α-androstanedione present in tissue and medium at the end of the incubation period. Superfusion experiments confirmed this estrogen effect and also showed a shift of the interconversion between A and T towards the reductive direction and a diminished tissue retention of DHT after estrogen treatment. These effects may contribute to the marked regression of the epididymal epithelium that was noted in the estrogen-treated patients, which is thought to be mainly the result of the inhibition of androgen biosynthesis caused by chemical hypophysectomy. © 1986.

Registro:

Documento: Artículo
Título:Androgen metabolism in the human epididymis. effect of in vivo estrogen administration
Autor:Vazquez, M.H.; de Larminat, M.-A.; Gurpide, E.; Scorticati, C.; Blaquier, J.A.
Filiación:Instituto de Biología y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Department of Obstetrics, Gynecology and Reproductive Science, Mount Sinai School of Medicine, New YorkU.S.A.
Instituto de Oncologia Angel Roffb, Universidad de Buenos Aires, Argentina
Palabras clave:androgen; androstanediol; androstanedione; androstanolone; androstenedione c 14; diethylstilbestrol; estrogen; radioisotope; steroid 5alpha reductase; testosterone; testosterone h 3; unclassified drug; adverse drug reaction; article; drug analysis; drug identification; drug mechanism; drug metabolism; drug monitoring; epididymis; histology; human; human cell; male genital system; priority journal; therapy; Aged; Androgens; Androstenedione; Epididymis; Estrogens; Humans; Male; Middle Aged; Testosterone
Año:1986
Volumen:25
Número:2
Página de inicio:239
Página de fin:244
DOI: http://dx.doi.org/10.1016/0022-4731(86)90422-X
Título revista:Journal of Steroid Biochemistry
Título revista abreviado:J. Steroid Biochem.
ISSN:00224731
CODEN:JSTBB
CAS:androstanediol, 1852-53-5, 25126-76-5, 67332-38-1; androstanedione, 5982-99-0, 846-46-8; androstanolone, 521-18-6; diethylstilbestrol, 30498-85-2, 56-53-1; testosterone, 58-22-0; Androgens; Androstenedione, 63-05-8; Estrogens; Testosterone, 58-22-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v25_n2_p239_Vazquez

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Citas:

---------- APA ----------
Vazquez, M.H., de Larminat, M.-A., Gurpide, E., Scorticati, C. & Blaquier, J.A. (1986) . Androgen metabolism in the human epididymis. effect of in vivo estrogen administration. Journal of Steroid Biochemistry, 25(2), 239-244.
http://dx.doi.org/10.1016/0022-4731(86)90422-X
---------- CHICAGO ----------
Vazquez, M.H., de Larminat, M.-A., Gurpide, E., Scorticati, C., Blaquier, J.A. "Androgen metabolism in the human epididymis. effect of in vivo estrogen administration" . Journal of Steroid Biochemistry 25, no. 2 (1986) : 239-244.
http://dx.doi.org/10.1016/0022-4731(86)90422-X
---------- MLA ----------
Vazquez, M.H., de Larminat, M.-A., Gurpide, E., Scorticati, C., Blaquier, J.A. "Androgen metabolism in the human epididymis. effect of in vivo estrogen administration" . Journal of Steroid Biochemistry, vol. 25, no. 2, 1986, pp. 239-244.
http://dx.doi.org/10.1016/0022-4731(86)90422-X
---------- VANCOUVER ----------
Vazquez, M.H., de Larminat, M.-A., Gurpide, E., Scorticati, C., Blaquier, J.A. Androgen metabolism in the human epididymis. effect of in vivo estrogen administration. J. Steroid Biochem. 1986;25(2):239-244.
http://dx.doi.org/10.1016/0022-4731(86)90422-X