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Abstract:

Samples containing as precursors either 18-hydroxycorticosterone (18-OH-B) in its M form, or this converted to less polar forms at pH 2 (ACM), or M or ACM enclosed in liposomes from adrenal lipids were incubated at pH 7.4, 4.8 or 3.3 in the presence or absence of quartered rat adrenals for 1 and 2 h. Optimal (10%) yields of aldosterone were obtained when (a) ACM was incubated at pH 4.8 and (b) M enclosed in liposomes was suspended in buffer and shaken without enzyme at pH 3.3. When conditions (a) were supplemented with malate and NADP, 16% of ACM was converted to aldosterone. ACM contained 80% of a fraction which, according to 13C NMR spectroscopy, was identical to 18-deoxyaldosterone (18-DAL). Experiments in which radioactivity from corticosterone (B) or M was trapped by radioinert M or 18-DAL disclosed a pathway comprising sequentially B, 18-OH-B, 18-DAL and aldosterone, and the combined evidence of this work, an enzymatic hydroxylation of 18-DAL to aldosterone. © 1985.

Registro:

Documento: Artículo
Título:Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
Autor:Cozza, E.N.; Burton, G.; Ceballos, N.R.; Lantos, C.P.; Harnik, M.; Scott, A.I.
Filiación:PRHOM and UMYMFOR (CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon 2, 1428 Buenos Aires, Argentina
Center for Biotechnology, the George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel
Department of Chemistry, Texas A and M University, College Station, TX 77843, United States
Palabras clave:18 deoxyaldosterone; 18 hydroxycorticosterone; aldosterone; radioisotope; unclassified drug; adrenal gland; animal cell; article; endocrine system; nonhuman; priority journal; rat; 18-Hydroxycorticosterone; Adrenal Glands; Aldosterone; Animals; Corticosterone; Hydrogen-Ion Concentration; Liposomes; Magnetic Resonance Spectroscopy; Male; Rats; Animalia
Año:1985
Volumen:22
Número:5
Página de inicio:665
Página de fin:672
DOI: http://dx.doi.org/10.1016/0022-4731(85)90221-3
Título revista:Journal of Steroid Biochemistry
Título revista abreviado:J. Steroid Biochem.
ISSN:00224731
CODEN:JSTBB
CAS:18 deoxyaldosterone, 2507-88-2; 18 hydroxycorticosterone, 561-65-9; aldosterone, 52-39-1, 6251-69-0; 18-Hydroxycorticosterone, 561-65-9; 18-deoxyaldosterone, 2507-88-2; Aldosterone, 52-39-1; Corticosterone, 50-22-6; Liposomes
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v22_n5_p665_Cozza

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Citas:

---------- APA ----------
Cozza, E.N., Burton, G., Ceballos, N.R., Lantos, C.P., Harnik, M. & Scott, A.I. (1985) . Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals. Journal of Steroid Biochemistry, 22(5), 665-672.
http://dx.doi.org/10.1016/0022-4731(85)90221-3
---------- CHICAGO ----------
Cozza, E.N., Burton, G., Ceballos, N.R., Lantos, C.P., Harnik, M., Scott, A.I. "Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals" . Journal of Steroid Biochemistry 22, no. 5 (1985) : 665-672.
http://dx.doi.org/10.1016/0022-4731(85)90221-3
---------- MLA ----------
Cozza, E.N., Burton, G., Ceballos, N.R., Lantos, C.P., Harnik, M., Scott, A.I. "Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals" . Journal of Steroid Biochemistry, vol. 22, no. 5, 1985, pp. 665-672.
http://dx.doi.org/10.1016/0022-4731(85)90221-3
---------- VANCOUVER ----------
Cozza, E.N., Burton, G., Ceballos, N.R., Lantos, C.P., Harnik, M., Scott, A.I. Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals. J. Steroid Biochem. 1985;22(5):665-672.
http://dx.doi.org/10.1016/0022-4731(85)90221-3