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Abstract:

The number of androgen (AR) and estrogen receptor (ER) binding sites was quantitated in cytosol fractions from adult intact male and female rats. The apparent equilibrium constants of dissociation were: 0.8 × 10−9 M for the AR measured with [3H]-R 1881 and 0.6 × 10−9 M for the ER labelled with [3H]-estradiol. The mean (±SD) number of available binding sites were (fmol/mg cytosol protein): male: AR: 88.6 ± 7.7 ER: 8.5 ± 1.4; female: AR: 23.8 ± 1.6 and ER: 7.6 ± 0.5. Orchidectomy Caused a two-fold increase in the number of available androgen and estrogen binding sites with a clear-cut reduction 10 days after castration. Ovariectomy elicited an unexpected decrease of ER one day after gonadectomy and full recovery of binding sites was found at 10 days. A significant increase in AR was seen 4 days following ovariectomy. © 1980, All rights reserved.

Registro:

Documento: Artículo
Título:Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland
Autor:Calandra, R.S.; Lüthy, I.; Finocchiaro, L.; Terrab, R.
Filiación:Laboratorio de Esteroides, Institute de Biología y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:androgen receptor; cytosol receptor; estradiol; estradiol h 3; estrogen receptor; hormone receptor; methyltrienolone h 3; metribolone; radioisotope; sex hormone; unclassified drug; adrenal gland; animal experiment; article; endocrine system; female genital system; gonadectomy; in vitro study; male genital system; orchiectomy; ovariectomy; preliminary communication; rat; sex difference; sex differentiation; Animalia
Año:1980
Volumen:13
Número:11
Página de inicio:1331
Página de fin:1335
DOI: http://dx.doi.org/10.1016/0022-4731(80)90094-1
Título revista:Journal of Steroid Biochemistry
Título revista abreviado:J. Steroid Biochem.
ISSN:00224731
CAS:estradiol, 50-28-2; metribolone, 965-93-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v13_n11_p1331_Calandra

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Citas:

---------- APA ----------
Calandra, R.S., Lüthy, I., Finocchiaro, L. & Terrab, R. (1980) . Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland. Journal of Steroid Biochemistry, 13(11), 1331-1335.
http://dx.doi.org/10.1016/0022-4731(80)90094-1
---------- CHICAGO ----------
Calandra, R.S., Lüthy, I., Finocchiaro, L., Terrab, R. "Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland" . Journal of Steroid Biochemistry 13, no. 11 (1980) : 1331-1335.
http://dx.doi.org/10.1016/0022-4731(80)90094-1
---------- MLA ----------
Calandra, R.S., Lüthy, I., Finocchiaro, L., Terrab, R. "Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland" . Journal of Steroid Biochemistry, vol. 13, no. 11, 1980, pp. 1331-1335.
http://dx.doi.org/10.1016/0022-4731(80)90094-1
---------- VANCOUVER ----------
Calandra, R.S., Lüthy, I., Finocchiaro, L., Terrab, R. Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland. J. Steroid Biochem. 1980;13(11):1331-1335.
http://dx.doi.org/10.1016/0022-4731(80)90094-1