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Abstract:

The in vitro activity of epididymal 5α-reductase and 3-hydroxy-steroid dehydrogenase was studied in rats during sexual development (ages 15 to 50 days). It was found that total activity (per organ) of 3-hydroxysteroid dehydrogenase reached an absolute maximum in 25 day-old rats. At this age there was also a marked increase in the specific activity (per mg weight) of both enzymes studied. While at all other ages 5α-DHT was the main product from testosterone, in the 25 day-old rat, 5α-androstandiol accounted for 73% of the metabolites formed. The subcellular distribution of both enzymes in the epididymides of adult and prepuberal rats was found to present only minor differences. Kinetic parameters (KM and Vmax) were measured in the subcellular fractions of the immature epididymis. These results indicate the existence of a marked, but transient, shift in the activity of the enzymes which metabolize androgens in the epididymis, towards the formation of 5α-androstandiol during the discrete period between 20 and 30 days of life. © 1980, All rights reserved.

Registro:

Documento: Artículo
Título:The in vitro metabolism of androgens in rat epididymis during sexual development
Autor:De Larminat, M.A.; Cuasnicú, P.S.; Blaquier, J.A.
Filiación:Instituto de Biología y Medicana Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:3(or 17)beta hydroxysteroid dehydrogenase; androgen; androstanediol h 3; androstanolone c 14; androstanolone h 3; radioisotope; steroid reductase; testosterone c 14; testosterone h 3; unclassified drug; age; animal experiment; article; epididymis; hormone metabolism; infant; male genital system; preliminary communication; rat; sexual development; Animalia
Año:1980
Volumen:13
Número:10
Página de inicio:1207
Página de fin:1213
DOI: http://dx.doi.org/10.1016/0022-4731(80)90076-X
Título revista:Journal of Steroid Biochemistry
Título revista abreviado:J. Steroid Biochem.
ISSN:00224731
CAS:3(or 17)beta hydroxysteroid dehydrogenase, 9015-81-0; steroid reductase, 42616-29-5, 9082-73-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v13_n10_p1207_DeLarminat

Referencias:

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Citas:

---------- APA ----------
De Larminat, M.A., Cuasnicú, P.S. & Blaquier, J.A. (1980) . The in vitro metabolism of androgens in rat epididymis during sexual development. Journal of Steroid Biochemistry, 13(10), 1207-1213.
http://dx.doi.org/10.1016/0022-4731(80)90076-X
---------- CHICAGO ----------
De Larminat, M.A., Cuasnicú, P.S., Blaquier, J.A. "The in vitro metabolism of androgens in rat epididymis during sexual development" . Journal of Steroid Biochemistry 13, no. 10 (1980) : 1207-1213.
http://dx.doi.org/10.1016/0022-4731(80)90076-X
---------- MLA ----------
De Larminat, M.A., Cuasnicú, P.S., Blaquier, J.A. "The in vitro metabolism of androgens in rat epididymis during sexual development" . Journal of Steroid Biochemistry, vol. 13, no. 10, 1980, pp. 1207-1213.
http://dx.doi.org/10.1016/0022-4731(80)90076-X
---------- VANCOUVER ----------
De Larminat, M.A., Cuasnicú, P.S., Blaquier, J.A. The in vitro metabolism of androgens in rat epididymis during sexual development. J. Steroid Biochem. 1980;13(10):1207-1213.
http://dx.doi.org/10.1016/0022-4731(80)90076-X