Artículo
Tesone, M.; Chazenbalk, G.D.; Ballejos, G.; Charreau, E.H. "Estrogen receptor in rat pancreatic islets" (1979) Journal of Steroid Biochemistry. 11(3):1309-1314
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Abstract:
The cytosol fraction of pancreatic islets of the female rat was found to contain a specifically binding protein for [3H]-estradiol. This protein was heat sensitive and the [3H]-estradiol binding was eliminated by treatment with protease and sulphydryl-blocking agents. Scatchard analysis of the cytosol binding reaction, measured by charcoal-dextran assay, indicated a single class of estradiol-binding sites having high affinity (Kd = 2.9 × 10-8 M at 0°C). The number of binding sites was calculated to be 29.6 fmol/mg cytosol protein in whole pancreas and 170 fmol/mg cytosol protein in isolated pancreatic islets after collagenase treatment. Competition studies indicated high specificity for the binding reaction, since excess (100-fold) unlabelled estrogens, diethylstilbestrol and the antiestrogen nafoxidine, all significantly reduced the binding of [3H]-estradiol. On the other hand, the nonestrogenic steroids dihydrotestosterone, corticosterone and progesterone had no significant effects on [3H]-estradiol binding. The complex had a sedimentation coefficient of 4-5 S in sucrose density gradient centrifugation in low salt. In streptozotocin-diabetic and in 3-wk pregnant rats a significant decrease in the binding of estradiol to pancreas islet cytosol was found. © 1979.
Registro:
| Documento: | Artículo |
| Título: | Estrogen receptor in rat pancreatic islets |
| Autor: | Tesone, M.; Chazenbalk, G.D.; Ballejos, G.; Charreau, E.H. |
| Filiación: | Laboratorio de Esteroides, Instituto de Biologǐa y Medicina Experimental, Obligado 2490, (1428) Buenos AiresArgentina Departamento de Quǐmica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
| Palabras clave: | estradiol; estrogen receptor; animal; article; binding competition; castration; cytosol; experimental diabetes mellitus; female; isolation and purification; kinetics; metabolism; pancreas islet; pregnancy; rat; Animal; Binding, Competitive; Castration; Cytosol; Diabetes Mellitus, Experimental; Estradiol; Female; Islets of Langerhans; Kinetics; Pregnancy; Rats; Receptors, Estrogen |
| Año: | 1979 |
| Volumen: | 11 |
| Número: | 3 |
| Página de inicio: | 1309 |
| Página de fin: | 1314 |
| DOI: | http://dx.doi.org/10.1016/0022-4731(79)90201-2 |
| Título revista: | Journal of Steroid Biochemistry |
| Título revista abreviado: | J. Steroid Biochem. |
| ISSN: | 00224731 |
| CODEN: | JSTBB |
| CAS: | estradiol, 50-28-2; Estradiol, 50-28-2; Receptors, Estrogen |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v11_n3_p1309_Tesone |
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Citas:
---------- APA ----------
Tesone, M., Chazenbalk, G.D., Ballejos, G. & Charreau, E.H. (1979) . Estrogen receptor in rat pancreatic islets. Journal of Steroid Biochemistry, 11(3), 1309-1314.http://dx.doi.org/10.1016/0022-4731(79)90201-2
---------- CHICAGO ----------
Tesone, M., Chazenbalk, G.D., Ballejos, G., Charreau, E.H. "Estrogen receptor in rat pancreatic islets" . Journal of Steroid Biochemistry 11, no. 3 (1979) : 1309-1314.http://dx.doi.org/10.1016/0022-4731(79)90201-2
---------- MLA ----------
Tesone, M., Chazenbalk, G.D., Ballejos, G., Charreau, E.H. "Estrogen receptor in rat pancreatic islets" . Journal of Steroid Biochemistry, vol. 11, no. 3, 1979, pp. 1309-1314.http://dx.doi.org/10.1016/0022-4731(79)90201-2
---------- VANCOUVER ----------
Tesone, M., Chazenbalk, G.D., Ballejos, G., Charreau, E.H. Estrogen receptor in rat pancreatic islets. J. Steroid Biochem. 1979;11(3):1309-1314.http://dx.doi.org/10.1016/0022-4731(79)90201-2
