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Kohane, A.C.; Garberi, J.C.; Cameo, M.S.; Blaquier, J.A. "Quantitative determination of specific proteins in rat epididymis" (1979) Journal of Steroid Biochemistry. 11(1 PART 2):671-674
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Abstract:

This paper describes the development of a radioimmunoassay for the measurement of specific proteins (SEP) in rat epididymis. Using [125I]-SEP the assay is useful within the range 0.6-30 ng SEP. With this method we confirmed the organ specificity of SEP which could neither be detected in the cytosol of several other organs in the rat nor in rat blood plasma. Furthermore, SEP appear to be species specific since the epididymal cytosol from man, stallion, bull, dog and rabbit did not react with serum anti rat SEP. Castration decreased the SEP content of rat epididymis. Preincubation of anti-SEP with cauda epididymis spermatozoa sharply decreased the binding capacity of the antiserum. The same treatment did not alter the binding capacity of control anti-LH serum. Immunofluorescence experiments show that SEP are attached to spermatozoa obtained from the cauda epididymis. © 1979.

Registro:

Documento: Artículo
Título:Quantitative determination of specific proteins in rat epididymis
Autor:Kohane, A.C.; Garberi, J.C.; Cameo, M.S.; Blaquier, J.A.
Filiación:Instituto de Biología y Medicina Experimental, Laboratorio de Esteroides, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:protein; radioisotope; animal experiment; epididymis; male genital system; rat; Animal; Castration; Comparative Study; Epididymis; Fluorescent Antibody Technique; Immune Sera; Iodine Radioisotopes; Isotope Labeling; Male; Proteins; Radioimmunoassay; Rats; Tissue Distribution
Año:1979
Volumen:11
Número:1 PART 2
Página de inicio:671
Página de fin:674
DOI: http://dx.doi.org/10.1016/0022-4731(79)90098-0
Título revista:Journal of Steroid Biochemistry
Título revista abreviado:J. Steroid Biochem.
ISSN:00224731
CODEN:JSTBB
CAS:protein, 67254-75-5; Immune Sera; Iodine Radioisotopes; Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v11_n1PART2_p671_Kohane

Referencias:

  • Bedford, Maturation, transport and fate of spermatozoa in the epididymis (1975) Handbook of Physiology, 5, pp. 303-317. , D.W. Hamilton, R.O. Greep, American Physiological Society, Washington DC, section 7
  • Blaquier, Cameo, Burgos, The role of androgens in the maturation of epididymal spermatozoa in the guinea-pig (1972) Endocrinology, 90, pp. 839-842
  • Orgebin-Crist, Danzo, Davies, Endocrine control of the development and maintenance of sperm fertilizing ability in the epididymis (1975) Handbook of Physiology, 5, pp. 319-338. , D.W. Hamilton, R.O. Greep, American Physiological Society, Washington, DC, section 7
  • Hamilton, The epididymis (1977) Frontiers in Reproduction and Fertility Control, pp. 411-426. , R.O. Greep, M.A. Koblinsky, MIT Press, Cambridge, MA
  • Fournier, Repartition de l'acide sialique dans l'appareil genital du rat Wistar adulte normal castré (1966) Compte rendu Soc. Biol., 160, pp. 1087-1090
  • Koskimies, Kormano, Proteins in fluids from different segments of the rat epididymis (1975) J. reprod. Fertil., 43, pp. 345-348
  • Lea, Petruz, French, Purification and localization of acidic epididymal glycoprotein (AEG) A sperm coating protein secreted by the rat epididymis (1978) International Journal of Andrology, pp. 592-605. , part 2
  • Cameo, Blaquier, Androgen-controlled specific proteins in rat epididymis (1976) J. Endocr., 69, pp. 47-55
  • Orgebin-Crist, Jahad, The maturation of rabbit epididymal spermatozoa in organ culture: Inhibition by antiandrogens and inhibitors of ribonucleic acid and protein synthesis (1978) Endocrinology, 103, pp. 46-53
  • Garberi, Kohane, Cameo, Blaquier, Isolation and characterization of specific rat epididymal proteins (1979) Molec. cell Endocr., 13, pp. 73-82
  • Bonavida, Structural basis for immune recognition of lysozymes. IV Immunologically active peptide obtained by the action of cyanogen bromide on human lysozyme (1971) Immunochemistry, 8, pp. 829-839
  • Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. biol. Chem., 193, pp. 265-275
  • Olson, Hamilton, Characterization of the surface glycoproteins of rat spermatozoa (1978) Biol reprod., 19, pp. 26-35

Citas:

---------- APA ----------
Kohane, A.C., Garberi, J.C., Cameo, M.S. & Blaquier, J.A. (1979) . Quantitative determination of specific proteins in rat epididymis. Journal of Steroid Biochemistry, 11(1 PART 2), 671-674.
http://dx.doi.org/10.1016/0022-4731(79)90098-0
---------- CHICAGO ----------
Kohane, A.C., Garberi, J.C., Cameo, M.S., Blaquier, J.A. "Quantitative determination of specific proteins in rat epididymis" . Journal of Steroid Biochemistry 11, no. 1 PART 2 (1979) : 671-674.
http://dx.doi.org/10.1016/0022-4731(79)90098-0
---------- MLA ----------
Kohane, A.C., Garberi, J.C., Cameo, M.S., Blaquier, J.A. "Quantitative determination of specific proteins in rat epididymis" . Journal of Steroid Biochemistry, vol. 11, no. 1 PART 2, 1979, pp. 671-674.
http://dx.doi.org/10.1016/0022-4731(79)90098-0
---------- VANCOUVER ----------
Kohane, A.C., Garberi, J.C., Cameo, M.S., Blaquier, J.A. Quantitative determination of specific proteins in rat epididymis. J. Steroid Biochem. 1979;11(1 PART 2):671-674.
http://dx.doi.org/10.1016/0022-4731(79)90098-0