Artículo
Abstract:
The fertility of spermatozoa from the different epididymal segments of hamsters was tested by in-vivo insemination. Caput and proximal corpus spermatozoa were non-fertile; spermatozoa from the distal corpus epididymidis fertilized 13% (38/290) oocytes and those from the proximal and distal cauda epididymidis 71 and 87%, respectively. When tested by in-vitro insemination, distal corpus spermatozoa penetrated 44% of oocytes while those from the distal cauda fertilized 87% of oocytes. Spermatozoa from the distal corpus recovered in Medium BMOC fertilized 13% (28/210) of oocytes in vivo, while those mixed with an epididymal protein preparation (0.8 mg protein/ml) fertilized 24% (49/204; P<0.01) of oocytes. When distal corpus spermatozoa were inseminated in vivo with 0.8 mg epididymal protein preparation 34% (31/90) oocytes were fertilized and only 22% (23/103; P<0.05) oocytes were fertilized when the proteins were obtained from epididymides of animals castrated for 30 days. When distal corpus spermatozoa were preincubated for 5 h in medium without (control) or with protein preparation (0.8 or 1.6 mg protein/ml), a significant increase in in-vitro oocyte penetration was found (25 compared with 45%; P<0.05) when the protein was present at 1.6 mg/ml. These results confirm and extend previous observations suggesting a role for androgen-dependent glycoproteins secreted by the epididymis in the acquisition of fertilizing ability that occurs during sperm maturation.
Registro:
| Documento: | Artículo |
| Título: | Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro |
| Autor: | Gonzalez Echeverria, F.; Cuasnicu, P.S.; Piazza, A. |
| Filiación: | Instituto de Biologia y Medicina Experimental, 1428 Buenos Aires, Argentina |
| Palabras clave: | chorionic gonadotropin; animal experiment; drug efficacy; epididymis; epididymis protein; fertilization; gonadotropin blood level; in vitro study; male genital system; nonhuman; spermatozoon; Animal; Epididymis; Female; Fertilization; Fertilization in Vitro; Glycoproteins; Hamsters; Male; Mesocricetus; Sperm Maturation; Tissue Extracts |
| Año: | 1984 |
| Volumen: | 71 |
| Número: | 2 |
| Página de inicio: | 433 |
| Página de fin: | 437 |
| Título revista: | Journal of Reproduction and Fertility |
| Título revista abreviado: | J. REPROD. FERTIL. |
| ISSN: | 00224251 |
| CODEN: | JRPFA |
| CAS: | chorionic gonadotropin, 9002-61-3; Glycoproteins; Tissue Extracts |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p433_GonzalezEcheverria |
Citas:
---------- APA ----------
Gonzalez Echeverria, F., Cuasnicu, P.S. & Piazza, A. (1984) . Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro. Journal of Reproduction and Fertility, 71(2), 433-437.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p433_GonzalezEcheverria [ ]
---------- CHICAGO ----------
Gonzalez Echeverria, F., Cuasnicu, P.S., Piazza, A. "Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro" . Journal of Reproduction and Fertility 71, no. 2 (1984) : 433-437.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p433_GonzalezEcheverria [ ]
---------- MLA ----------
Gonzalez Echeverria, F., Cuasnicu, P.S., Piazza, A. "Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro" . Journal of Reproduction and Fertility, vol. 71, no. 2, 1984, pp. 433-437.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p433_GonzalezEcheverria [ ]
---------- VANCOUVER ----------
Gonzalez Echeverria, F., Cuasnicu, P.S., Piazza, A. Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro. J. REPROD. FERTIL. 1984;71(2):433-437.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p433_GonzalezEcheverria [ ]
