The increase in zona pellucida binding caused by the exposure of cultured proximal corpus epididymidis to 2 μM-5α-DHT (0.87 and 4.20 spermatozoa/egg for control and 5α-DHT group respectively) was lost when 20 μM-cycloheximide was also added to the medium (0.72 spermatozoa/egg). These results were interpreted as meaning that de-novo protein synthesis was required to obtain the effect of androgens. When a fraction enriched in epididymal glycoproteins (EP2-EP6 (18% total protein in epididymal cytosol and 30% in enriched fraction) and depleted of androgens (<120 pg testosterone + DHT/ml) was added to the cultured epididymal tubules, the zona pellucida-binding ability of the contained spermatozoa increased from 0.55 in controls to 2.73 spermatozoa/egg in the extract-treated group (P<0.02). When the enriched fraction was prepared from epididymides of 30-day castrates, the stimulatory effect was lost (1.04 spermatozoa/egg). We suggest that proteins synthesized in the epididymis are required to obtain the effect of androgens and that the glycoproteins EP2-EP6 may be involved.
Documento: | Artículo |
Título: | Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa |
Autor: | Cuasnicu, P.S.; Gonzalez Echeverria, F.; Piazza, A. |
Filiación: | Instituto de Biologia y Medicina Experimental, 1428 Buenos Aires, Argentina |
Palabras clave: | androgen; protein; animal cell; biological model; epididymis; female genital system; male genital system; nonhuman; spermatozoon; zona pellucida; Animalia; Cricetinae |
Año: | 1984 |
Volumen: | 71 |
Número: | 2 |
Página de inicio: | 427 |
Página de fin: | 431 |
DOI: | http://dx.doi.org/10.1530/jrf.0.0710427 |
Título revista: | Journal of Reproduction and Fertility |
Título revista abreviado: | J. REPROD. FERTIL. |
ISSN: | 00224251 |
CODEN: | JRPFA |
CAS: | protein, 67254-75-5 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p427_Cuasnicu |