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Abstract:

1. The effects of different calcium channel blockers ω-agatoxin IVA (ω-Aga IVA), ω-conotoxin GVIA (ω-CgTx GVIA) and dihydropyridines) were tested on spontaneous and evoked transmitter release at embryonic and newborn rat neuromuscular junctions (NMJs). 2. The nerve-evoked transmitter release quantal content (m) was strongly reduced by the P/Q-type voltage-dependent calcium channel (VDCC) blocker ω-Aga IVA (100 nM) at newly formed endplates of embryos and 0- to 11-day-old rats, in agreement with the effect of this blocker on transmitter release at mature and reinnervating muscles. 3. ω-CgTx GVIA (1-5 μM), the N-type VDCC blocker, also caused a significant reduction in m at newly formed NMJs early in development (embryos and 0- to 4-day-old rats), while it was ineffective in more mature animals (5- to 11-day-old rats). 4. L-type channel blockers, nitrendipine (1 μM) and nifedipine (1 μM), did not significantly affect neurally evoked release at developing NMJs. However, nifedipine (10 μM) was able to increase m significantly at 0- to 4-day-old rat NMJs. 5. At developing NMJs, K+-evoked transmitter release was dependent on Ca2+ entry through VDCCs of the P/Q-type family (100 nM ω-Aga IVA reduced 70% of the K+-evoked miniature endplate potential frequency). N- and L-type VDCC blockers did not affect this type of release. 6. We conclude that at rat neuromuscular junctions the presynaptic calcium channel types involved in transmitter release undergo developmental changes during the early postnatal period.

Registro:

Documento: Artículo
Título:Calcium channels coupled to neurotransmitter release at neonatal rat neuromuscular junctions
Autor:Rosato Siri, M.D.; Uchitel, O.D.
Filiación:Lab. de Fisiol. y Biol. Molecular, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires (1428), Argentina
Lab. de Fisiol. y Biol. Molecular, Cd. Univ. 2do piso, Buenos Aires (1428), Argentina
Palabras clave:calcium channel; calcium ion; dihydropyridine; neurotransmitter; nifedipine; nitrendipine; omega agatoxin IVA; omega conotoxin GVIA; potassium ion; calcium channel; unclassified drug; voltage dependent calcium channel; animal cell; article; calcium transport; controlled study; embryo; embryo development; miniature endplate potential; muscle reinnervation; neuromuscular synapse; neurotransmitter release; newborn; nonhuman; perinatal development; priority journal; rat; animal tissue; Article; nerve potential; postnatal development; protein function; stimulus response; synaptic transmission; Animals; Animals, Newborn; Calcium Channel Blockers; Calcium Channels; Calcium Channels, L-Type; Calcium Channels, N-Type; Diaphragm; Embryo; Evoked Potentials; Motor Endplate; Muscle, Skeletal; Neuromuscular Junction; Neurotransmitter Agents; Nifedipine; Nitrendipine; omega-Agatoxin IVA; omega-Conotoxin GVIA; Peptides; Phrenic Nerve; Rats; Rats, Wistar; Spider Venoms; Synaptic Transmission
Año:1999
Volumen:514
Número:2
Página de inicio:533
Página de fin:540
DOI: http://dx.doi.org/10.1111/j.1469-7793.1999.533ae.x
Título revista:Journal of Physiology
Título revista abreviado:J. Physiol.
ISSN:00223751
CODEN:JPHYA
CAS:Calcium Channel Blockers; Calcium Channels; Calcium Channels, L-Type; Calcium Channels, N-Type; Neurotransmitter Agents; Nifedipine, 21829-25-4; Nitrendipine, 39562-70-4; omega-Agatoxin IVA; omega-Conotoxin GVIA, 92078-76-7; Peptides; Spider Venoms; voltage-dependent calcium channel (P-Q type)
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223751_v514_n2_p533_RosatoSiri

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Citas:

---------- APA ----------
Rosato Siri, M.D. & Uchitel, O.D. (1999) . Calcium channels coupled to neurotransmitter release at neonatal rat neuromuscular junctions. Journal of Physiology, 514(2), 533-540.
http://dx.doi.org/10.1111/j.1469-7793.1999.533ae.x
---------- CHICAGO ----------
Rosato Siri, M.D., Uchitel, O.D. "Calcium channels coupled to neurotransmitter release at neonatal rat neuromuscular junctions" . Journal of Physiology 514, no. 2 (1999) : 533-540.
http://dx.doi.org/10.1111/j.1469-7793.1999.533ae.x
---------- MLA ----------
Rosato Siri, M.D., Uchitel, O.D. "Calcium channels coupled to neurotransmitter release at neonatal rat neuromuscular junctions" . Journal of Physiology, vol. 514, no. 2, 1999, pp. 533-540.
http://dx.doi.org/10.1111/j.1469-7793.1999.533ae.x
---------- VANCOUVER ----------
Rosato Siri, M.D., Uchitel, O.D. Calcium channels coupled to neurotransmitter release at neonatal rat neuromuscular junctions. J. Physiol. 1999;514(2):533-540.
http://dx.doi.org/10.1111/j.1469-7793.1999.533ae.x