Abstract:
1. The involvement of the different types of voltage-dependent calcium channels (VDCCs) in synaptic transmission at the mature and newly formed mammalian neuromuscular junction was studied by evaluating the effects of L-, P/Q- and N-type VDCC antagonists on transmitter release in normal and reinnervating levator auris preparations of adult mice. 2. Nerve-evoked transmitter release was blocked by ω-agatoxin IVA (ω-AgaIVA), a P/Q-type VDCC blocker, both in normal and reinnervating endplates (100 nM ω-AgaIVA caused > 90% inhibition). The N-type VDCC antagonist ω-conotoxin GVIA (ω-CgTX; 1 and 5 μM), as occurs in normal preparations, did not significantly affect this type of release during reinnervation. Nitrendipine (1-10 μM), an L-type VDCC blocker, strongly antagonized release in reinnervating muscles (~ 40-60% blockade) and lacked any effect in normal preparations. 3. In reinnervating muscles, spontaneous release was not dependent on Ca2+ entry through either P- or L-type VDCCs. Neither 100 nM ω-AgaIVA nor 10 μM nitrendipine affected the miniature endplate potential (MEPP) frequency or amplitude. 4. At the newly formed endplates, K+-evoked release was dependent on Ca2+ entry through VDCCs of the P-type family (100 nM ω-AgaIVA reduced ~ 70% of the K+-evoked MEPP frequency). L-type VDCCs were found not to participate in this type of release (10 μM nitrendipine lacked any effect). 5. In reinnervating muscles, the L-type VDCC blocker, nitrendipine (10 μM), provoked a significant increase (~ 25%) in the latency of the evoked endplate potential (EPP). This drug also caused an increase (~ 0.3 ms) in the latency of the presynaptic currents. The P/Q- and N-type VDCC) blockers did not affect the latency of the EPP. 6. These results show that at newly formed mouse neuromuscular junctions, as occurs in mature preparations, VDCCs of the P-type family play a prominent role in evoked transmitter release whereas N-type channels are not involved in this process. In addition, signal conduction and transmitter release become highly sensitive to nitrendipine during reinnervation. This suggests that L-type VDCCs may play a role in synaptic transmission at the immature mammalian neuromuscular junction.
Registro:
Documento: |
Artículo
|
Título: | Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction |
Autor: | Katz, E.; Ferro, P.A.; Weisz, G.; Uchitel, O.D. |
Filiación: | Inst. Biol. Cel. y Neurociencias P., Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires (1121), Argentina Departamento de Biología, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires (1428), Argentina
|
Palabras clave: | calcium channel; conotoxin; nitrendipine; potassium ion; toxin; animal tissue; article; calcium transport; controlled study; endplate potential; latent period; male; membrane current; miniature endplate potential; mouse; muscle reinnervation; nervous system development; neuromuscular synapse; neurotransmitter release; nonhuman; presynaptic nerve; priority journal; synaptic transmission |
Año: | 1996
|
Volumen: | 497
|
Número: | 3
|
Página de inicio: | 687
|
Página de fin: | 697
|
DOI: |
http://dx.doi.org/10.1113/jphysiol.1996.sp021800 |
Título revista: | Journal of Physiology
|
Título revista abreviado: | J. PHYSIOL.
|
ISSN: | 00223751
|
CODEN: | JPHYA
|
CAS: | nitrendipine, 39562-70-4; potassium ion, 24203-36-9
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223751_v497_n3_p687_Katz |
Referencias:
- Angaut-Petit, D., Mallart, A., Ionic channel distribution in regenerating mouse motor endings (1985) Journal de Physiologie, 80, pp. 307-311
- Angaut-Petit, D., Mallart, A., Electrical activity of mouse motor endings during muscle reinnervation (1985) Neuroscience, 16, pp. 1047-1056
- Angelov, D.N., Neiss, W.F., Streppel, M., Andermahr, J., Mader, K., Stennert, E., Nimodipine accelerates axonal sprouting after surgical repair of rat facial nerve (1996) Journal of Neuroscience, 16, pp. 1041-1048
- Atchinson, W.D., Dihydropyridine-sensitive and -insensitive components of acetylcholine release from rat motor nerve terminals (1989) Journal of Pharmacology and Experimental Therapeutics, 251, pp. 672-678
- Augustine, G.J., Regulation of transmitter release at the squid giant synapse by presynaptic delayed rectifier potassium current (1990) Journal of Physiology, 431, pp. 343-364
- Bennett, M.R., Mclachlan, E.M., Taylor, R.S., The formation of synapses in reinnervating mammalian striated muscle (1973) Journal of Physiology, 233, pp. 481-500
- Bostock, H., Sears, T.A., Sherratt, R.M., The effects of 4-aminopyridine and tetraethylammonium ions on normal and demyelinated mammalian nerve fibres (1981) Journal of Physiology, 313, pp. 301-315
- Bowersox, S.S., Miljanich, G.P., Sugiura, Y., Li, C., Nadasdi, L., Hoffman, B.B., Ramachandran, J., Ko, C., Differential blockade of voltage-sensitive calcium channels at the mouse neuromuscular junction by novel ω-conopeptides and ω-Agatoxin-IVA (1995) Journal of Pharmacology and Experimental Therapeutics, 273, pp. 248-256
- Del Castillo, J., Katz, B., Changes in end-plate activity produced by presynaptic polarization (1954) Journal of Physiology, 124, pp. 586-604
- Dennis, M.J., Miledi, R., Non-transmitting neuromuscular junctions during an early stage of end-plate reinnervation (1974) Journal of Physiology, 239, pp. 553-570
- Dennis, M.J., Miledi, R., Characteristics of transmitter release at regenerating frog neuromuscular junctions (1974) Journal of Physiology, 239, pp. 571-594
- Dodge, F.A., Rahamimoff, R., Co-operative action of calcium ions in transmitter release at the neuromuscular junction (1967) Journal of Physiology, 193, pp. 419-432
- Fu, W.M., Huang, F.L., L-type Ca2+ channel is involved in the regulation of spontaneous transmitter release at developing neuromuscular synapses (1994) Neuroscience, 58, pp. 131-140
- Gray, D.B., Brusés, J.L., Pilar, G.R., Developmental switch in the pharmacology of Ca2+ channels coupled to acetylcholine release (1992) Neuron, 8, pp. 1-20
- Hong, S.J., Chang, C.C., Inhibition of acetylcholine release from mouse motor nerves by a P-type calcium channel blocker, ω-agatoxin IVA (1995) Journal of Physiology, 482, pp. 283-290
- Hubbard, J.I., Llinás, R., Quastel, D.M.J., (1969) Electrophysiological Analysis of Synaptic Transmission, , Edward Arnold (Publishers) Ltd., London
- Katz, B., The Release of Neural Transmitter Substances (1969) Sherrington Lectures, 10. , Liverpool University Press, Liverpool
- Katz, B., Miledi, R., Further study of the role of calcium in synaptic transmission (1970) Journal of Physiology, 207, pp. 789-801
- Katz, E., Ferro, P.A., Weisz, G., Uchitel, O.D., Pharmacological characterization of the calcium channels involved in transmitter release at reinnervating mouse neuromuscular junctions (1995) Society for Neuroscience Abstracts, 140 (15), p. 340
- Llinás, R., Steinberg, I.Z., Walton, K., Presynaptic calcium currents and their relation to synaptic transmission: Voltage clamp study in squid giant synapse and theoretical model for the calcium gate (1976) Proceedings of the National Academy of Sciences of the USA, 73, pp. 2918-2922
- Llinás, R., Sugimori, M., Hillman, D.E., Cherksey, B.D., Distribution and functional significance of the P-type, voltage-dependent Ca2+ channels in the mammalian central nervous system (1992) Trends in Neurosciences, 15, pp. 351-355
- Lüscher, C., Lipp, P., Lüscher, H.-R., Niggli, E., Control of action potential propagation by intracellular Ca2+ in cultured rat dorsal root ganglion cells (1996) Journal of Physiology, 490, pp. 319-324
- Mclachlan, E.M., Martin, A.R., Non-linear summation of end-plate potentials in the frog and mouse (1981) Journal of Physiology, 311, pp. 307-324
- Mallart, A., Electric current flow inside perineurial sheaths of mouse motor nerves (1985) Journal of Physiology, 368, pp. 565-575
- Martin, A.R., Quantal nature of synaptic transmission (1966) Physiological Reviews, 46, pp. 51-66
- Mintz, I.M., Sabatini, B.L., Regehr, W.G., Calcium control of transmitter release at a cerebellar synapse (1995) Neuron, 15, pp. 675-688
- Mintz, I.M., Venema, V.J., Swiderek, K.M., Lee, T.D., Bean, B.P., Adams, M.E., P-type calcium channels blocked by the spider toxin ω-Aga-IVA (1992) Nature, 355, pp. 827-829
- Momiyama, A., Takahashi, T., Calcium channels responsible for potassium-induced transmitter release at rat cerebellar synapses (1994) Journal of Physiology, 476, pp. 197-202
- Nowicky, M.C., Fox, A.P., Tsien, R.W., Three types of neuronal calcium channels with different calcium agonist sensitivity (1985) Nature, 316, pp. 440-443
- Olivera, B.M., Miljanich, G.P., Ramachandran, J., Adams, M.E., Calcium channel diversity and neurotransmitter release: The ω-Conotoxins and ω-Agatoxins (1994) Annual Review of Biochemistry, 63, pp. 823-867
- Pearson, H.A., Sutton, K.G., Scott, R.H., Dolphin, A.C., Characterization of Ca2+ channels currents in cultured rat cerebellar granule neurones (1995) Journal of Physiology, 482, pp. 493-509
- Penner, R., Dreyer, F., Two different presynaptic calcium currents in mouse motor nerve terminals (1986) Pflügers Archiv, 406, pp. 190-197
- Protti, D.A., Reisin, R., Angelillo Mackinley, T., Uchiteil, O.D., Calcium channel blockers and transmitter release at the normal human neuromuscular junction (1996) Neurology, 46, pp. 1391-1396
- Protti, D.A., Szczupak, L., Scornik, F.S., Uchitel, O.D., Effect of ω-Conotoxin GVIA on neurotransmitter release at the mouse neuromuscular junction (1991) Brain Research, 557, pp. 336-339
- Protti, D.A., Uchitel, O.D., Transmitter release and presynaptic Ca2+ currents blocked by the spider toxin ω-Aga-IVA (1993) NeuroReport, 5, pp. 333-336
- Spitzer, N.C., Development of voltage-dependent and ligand gated channels in excitable membranes (1994) Progress in Brain Research, 102, pp. 169-179
- Tonge, D.A., Physiological characteristics of re-innervation of skeletal muscle in the mouse (1974) Journal of Physiology, 241, pp. 141-153
- Uchitel, O.D., Protti, D.A., Sánchez, V., Cherksey, B.D., Sugimori, M., Llinás, R., P-type voltage-dependent calcium channel mediates presynaptic calcium influx and transmitter release in mammalian synapses (1992) Proceedings of the National Academy of Sciences of the USA, 89, pp. 3330-3333
- Wessler, I., Dooley, D.J., Lohr, B., P-type Ca2+ channels trigger stimulus-evoked [3H]acetylcholine release from mammalian motor endplates (1995) European Journal of Pharmacology, 278, pp. 83-86
- Yatani, A., Brown, A.M., The calcium channel blocker nitrendipine blocks sodium channels in neonatal rat cardiac myocytes (1985) Circulation Research, 57, pp. 868-875
- Zhang, J.F., Randall, A.D., Ellinor, P.T., Horne, W.A., Sather, W.A., Tanabe, T., Schwarz, T.L., Tsien, R.W., Distinctive pharmacology and kinetics of cloned neuronal Ca2+ channels and their possible counterparts in mammalian CNS neurons (1993) Neuropharmacology, 32, pp. 1075-1088
- Zhu, P., Vrbová, G., The role of Ca2+ in the elimination of polyneuronal innervation of rat soleus muscle fibres (1992) European Journal of Neuroscience, 4, pp. 433-437
Citas:
---------- APA ----------
Katz, E., Ferro, P.A., Weisz, G. & Uchitel, O.D.
(1996)
. Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction. Journal of Physiology, 497(3), 687-697.
http://dx.doi.org/10.1113/jphysiol.1996.sp021800---------- CHICAGO ----------
Katz, E., Ferro, P.A., Weisz, G., Uchitel, O.D.
"Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction"
. Journal of Physiology 497, no. 3
(1996) : 687-697.
http://dx.doi.org/10.1113/jphysiol.1996.sp021800---------- MLA ----------
Katz, E., Ferro, P.A., Weisz, G., Uchitel, O.D.
"Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction"
. Journal of Physiology, vol. 497, no. 3, 1996, pp. 687-697.
http://dx.doi.org/10.1113/jphysiol.1996.sp021800---------- VANCOUVER ----------
Katz, E., Ferro, P.A., Weisz, G., Uchitel, O.D. Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction. J. PHYSIOL. 1996;497(3):687-697.
http://dx.doi.org/10.1113/jphysiol.1996.sp021800