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Abstract:

1. The involvement of the different types of voltage-dependent calcium channels (VDCCs) in synaptic transmission at the mature and newly formed mammalian neuromuscular junction was studied by evaluating the effects of L-, P/Q- and N-type VDCC antagonists on transmitter release in normal and reinnervating levator auris preparations of adult mice. 2. Nerve-evoked transmitter release was blocked by ω-agatoxin IVA (ω-AgaIVA), a P/Q-type VDCC blocker, both in normal and reinnervating endplates (100 nM ω-AgaIVA caused > 90% inhibition). The N-type VDCC antagonist ω-conotoxin GVIA (ω-CgTX; 1 and 5 μM), as occurs in normal preparations, did not significantly affect this type of release during reinnervation. Nitrendipine (1-10 μM), an L-type VDCC blocker, strongly antagonized release in reinnervating muscles (~ 40-60% blockade) and lacked any effect in normal preparations. 3. In reinnervating muscles, spontaneous release was not dependent on Ca2+ entry through either P- or L-type VDCCs. Neither 100 nM ω-AgaIVA nor 10 μM nitrendipine affected the miniature endplate potential (MEPP) frequency or amplitude. 4. At the newly formed endplates, K+-evoked release was dependent on Ca2+ entry through VDCCs of the P-type family (100 nM ω-AgaIVA reduced ~ 70% of the K+-evoked MEPP frequency). L-type VDCCs were found not to participate in this type of release (10 μM nitrendipine lacked any effect). 5. In reinnervating muscles, the L-type VDCC blocker, nitrendipine (10 μM), provoked a significant increase (~ 25%) in the latency of the evoked endplate potential (EPP). This drug also caused an increase (~ 0.3 ms) in the latency of the presynaptic currents. The P/Q- and N-type VDCC) blockers did not affect the latency of the EPP. 6. These results show that at newly formed mouse neuromuscular junctions, as occurs in mature preparations, VDCCs of the P-type family play a prominent role in evoked transmitter release whereas N-type channels are not involved in this process. In addition, signal conduction and transmitter release become highly sensitive to nitrendipine during reinnervation. This suggests that L-type VDCCs may play a role in synaptic transmission at the immature mammalian neuromuscular junction.

Registro:

Documento: Artículo
Título:Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction
Autor:Katz, E.; Ferro, P.A.; Weisz, G.; Uchitel, O.D.
Filiación:Inst. Biol. Cel. y Neurociencias P., Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires (1121), Argentina
Departamento de Biología, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires (1428), Argentina
Palabras clave:calcium channel; conotoxin; nitrendipine; potassium ion; toxin; animal tissue; article; calcium transport; controlled study; endplate potential; latent period; male; membrane current; miniature endplate potential; mouse; muscle reinnervation; nervous system development; neuromuscular synapse; neurotransmitter release; nonhuman; presynaptic nerve; priority journal; synaptic transmission
Año:1996
Volumen:497
Número:3
Página de inicio:687
Página de fin:697
DOI: http://dx.doi.org/10.1113/jphysiol.1996.sp021800
Título revista:Journal of Physiology
Título revista abreviado:J. PHYSIOL.
ISSN:00223751
CODEN:JPHYA
CAS:nitrendipine, 39562-70-4; potassium ion, 24203-36-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223751_v497_n3_p687_Katz

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Citas:

---------- APA ----------
Katz, E., Ferro, P.A., Weisz, G. & Uchitel, O.D. (1996) . Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction. Journal of Physiology, 497(3), 687-697.
http://dx.doi.org/10.1113/jphysiol.1996.sp021800
---------- CHICAGO ----------
Katz, E., Ferro, P.A., Weisz, G., Uchitel, O.D. "Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction" . Journal of Physiology 497, no. 3 (1996) : 687-697.
http://dx.doi.org/10.1113/jphysiol.1996.sp021800
---------- MLA ----------
Katz, E., Ferro, P.A., Weisz, G., Uchitel, O.D. "Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction" . Journal of Physiology, vol. 497, no. 3, 1996, pp. 687-697.
http://dx.doi.org/10.1113/jphysiol.1996.sp021800
---------- VANCOUVER ----------
Katz, E., Ferro, P.A., Weisz, G., Uchitel, O.D. Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction. J. PHYSIOL. 1996;497(3):687-697.
http://dx.doi.org/10.1113/jphysiol.1996.sp021800