Artículo

Bordenave, M.D.; Balzarotti, F.; Stefani, F.D.; Hell, S.W. "STED nanoscopy with wavelengths at the emission maximum" (2016) Journal of Physics D: Applied Physics. 49(36)
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Abstract:

Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate that continuous wave (CW) STED nanoscopy is feasible using STED wavelengths located at the emission maximum, where the cross section for stimulated emission is up to 10-fold larger than at the red tail. As a result, STED imaging becomes possible at equally lower STED beam power. Besides, fluorophores that have been considered inapplicable in certain wavelength constellations are thus becoming usable. © 2016 IOP Publishing Ltd.

Registro:

Documento: Artículo
Título:STED nanoscopy with wavelengths at the emission maximum
Autor:Bordenave, M.D.; Balzarotti, F.; Stefani, F.D.; Hell, S.W.
Filiación:Centro de Investigaciones en Bionanociencas (CIBION), Consejo Nacional de Investigaciones Científicas y Té Cnicas (CONICET), Godoy Cruz 2390, Buenos Aires, C1425FQD, Argentina
Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón, 1, Ciudad Universitaria, Buenos Aires, C1428EHA, Argentina
Department of Nanobiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
Palabras clave:acousto-optical devices; fluorescence microscopy; nanoscopy; resolution; superresolution; Acoustooptical devices; Emission spectroscopy; Fluorescence; Fluorescence microscopy; Optical resolving power; Stimulated emission; Beam power; Continuous waves; Emission maxima; Emission spectrums; nanoscopy; Stimulated emission depletion; Sub-diffraction; Super resolution; Fluorophores
Año:2016
Volumen:49
Número:36
DOI: http://dx.doi.org/10.1088/0022-3727/49/36/365102
Título revista:Journal of Physics D: Applied Physics
Título revista abreviado:J Phys D
ISSN:00223727
CODEN:JPAPB
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223727_v49_n36_p_Bordenave

Referencias:

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Citas:

---------- APA ----------
Bordenave, M.D., Balzarotti, F., Stefani, F.D. & Hell, S.W. (2016) . STED nanoscopy with wavelengths at the emission maximum. Journal of Physics D: Applied Physics, 49(36).
http://dx.doi.org/10.1088/0022-3727/49/36/365102
---------- CHICAGO ----------
Bordenave, M.D., Balzarotti, F., Stefani, F.D., Hell, S.W. "STED nanoscopy with wavelengths at the emission maximum" . Journal of Physics D: Applied Physics 49, no. 36 (2016).
http://dx.doi.org/10.1088/0022-3727/49/36/365102
---------- MLA ----------
Bordenave, M.D., Balzarotti, F., Stefani, F.D., Hell, S.W. "STED nanoscopy with wavelengths at the emission maximum" . Journal of Physics D: Applied Physics, vol. 49, no. 36, 2016.
http://dx.doi.org/10.1088/0022-3727/49/36/365102
---------- VANCOUVER ----------
Bordenave, M.D., Balzarotti, F., Stefani, F.D., Hell, S.W. STED nanoscopy with wavelengths at the emission maximum. J Phys D. 2016;49(36).
http://dx.doi.org/10.1088/0022-3727/49/36/365102