Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Cell wall chemistry in the coencocytic green seaweed Codium vermilara (Olivi) Delle Chiaje (Bryopsidales, Chlorophyta) is well understood. These cell walls are composed of major amounts of neutral β-(1→4)-D-mannans (Mn), sulfated polysaccharides (SPs), which include pyranosic arabinan sulfates (ArpS), pyruvylated galactan sulfates (pGaS), and mannan sulfates (MnS); also minor amounts of O-glycoproteins are present. In this study, cell wall samples of C. vermilara were investigated with regard to their monosaccharide composition and infrared spectra (using Fourier transform infrared spectroscopy coupled to principal component [FTIR-PC] analysis). Samples from three different populations of C. vermilara from the Argentine coast showed: (i) an important variation in the relative arabinan content, which increases from north to south, and (ii) a measurable degree of cell wall variability in the sulfate distribution between the different sulfated polysaccharides, independent of the amount of each polysaccharide present and of total sulfate content. When cell wall composition was analyzed over three consecutive years in a single geographic location, the quantity of Mn and overall sulfate content on SPs remained constant, whereas the pGaS:ArpS molar ratio changed over the time. Besides, similar cell wall composition was found between actively growing and resting zones of the thallus, suggesting that cell wall composition is independent of growth stage and development. Overall, these results suggest that C. vermilara has developed a mechanism to adjust the total level of cell wall sulfation by modulating the ArpS:pGaS:MnS molar ratio and also by adjusting the sulfation level in each type of polymer, whereas nonsulfated Mn, as the main structural polysaccharide, did not change over the time or growing stage. © 2011 Phycological Society of America.

Registro:

Documento: Artículo
Título:Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast
Autor:Fernández, P.V.; Ciancia, M.; Estevez, J.M.
Filiación:Cátedra de Química de Biomoléculas, Departamento de Biología Aplicada y Alimentos (CIHIDECAR-CONICET), Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular (IFIByNE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria - Pabellón 2, 1428 Buenos Aires, Argentina
National Research Council of Argentina (CONICET), Argentina
Palabras clave:β-mannans; Cell wall; Codium vermilara; Green seaweed; Sulfated polysaccharides; Temporal and geographic variation; Caulerpales; Chlorophyta; Codium vermilara
Año:2011
Volumen:47
Número:4
Página de inicio:802
Página de fin:810
DOI: http://dx.doi.org/10.1111/j.1529-8817.2011.01006.x
Título revista:Journal of Phycology
Título revista abreviado:J. Phycol.
ISSN:00223646
CODEN:JPYLA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v47_n4_p802_Fernandez

Referencias:

  • Ahmed, A.E.R., Labavitch, J.M., A simplified method for accurate determination of cell wall uronide content (1977) J. Food Biochem., 1, pp. 61-365
  • Aquino, R.S., Landeira-Fernandez, A.M., Occurrence of sulfated galactans in marine angiosperms: evolutionary implications (2005) Glycobiology, 15, pp. 11-20
  • Bilan, M.I., Vinogradova, E.V., Shashkov, A.S., Usov, A.I., Structure of a highly pyruvylated galactan sulfate from the pacific green alga Codium yezoense (Bryopsidales, Chlorophyta) (2007) Carbohydr. Res., 342, pp. 586-596
  • Boraso de Zaixso, A.L., Marine Chlorophyta of Argentina (2004) Hist. Nat. (Segunda Ser.), 3, pp. 95-119
  • Bouarab, K., Potin, P., Correa, J., Kloareg, B., Sulfated oligosaccharides mediate the interaction between a marine red alga and its green algal pathogenic endophyte (1999) Plant Cell, 11, pp. 1635-1650
  • Chen, L., Carpita, N.C., Reiter, W.-D., Wilson, R.H., Jeffries, C., McCann, M.C., A rapid method to screen for cell-wall mutants using discriminant analysis of Fourier transform infrared spectra (1998) Plant J., 16, pp. 385-392
  • Chirapart, A., Ohno, M., Seasonal variation in the physical properties of agar and biomass of Gracilaria sp (chorda type) from Tosa Bay, southern Japan (1993) Hydrobiologia, 260-261, pp. 541-547
  • Chopin, T., Whalen, E., A new and rapid method for carrageenan identification by FTIR diffuse reflectance spectroscopy directly on dried, ground algal material (1993) Carbohydr. Res., 246, pp. 51-59
  • Ciancia, M., Quintana, I., Vizcarguenaga, M.I., Kasulin, L., Dios, A., Estevez, J.M., Cerezo, A.S., Polysaccharides from the green seaweeds Codium fragile and C. vermilara with controversial effects on hemostasis (2007) Int. J. Biol. Macromol., 41, pp. 641-649
  • Dodgson, K.S., Price, R.G., A note on the determination of the ester sulphate content of sulphated polysaccharides (1962) Biochem. J., 84, pp. 106-110
  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method for the determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356
  • Estevez, J.M., Kasulin, L., Fernández, P.V., Dupree, P., Ciancia, M., Chemical and in situ characterization of macromolecular components of the complex cell walls from the coencocytic green alga Codium fragile (2009) Glycobiology, 19, pp. 212-222
  • Farias, E.H.C., Pomin, V.H., Valente, A.P., Nader, H.B., Rocha, H.A.O., Mourao, P.A.S., A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum (2008) Glycobiology, 18, pp. 250-259
  • Fernández, P.V., Ciancia, M., Miravalles, A.B., Estevez, J.M., Cell-wall polymer mapping in the coenocytic macroalga Codium vermilara (Bryopsidales, Cholorphyta) (2010) J. Phycol., 46, pp. 456-465
  • Givernaud, T.E.L., Gourji, A., Mouradi-Givernaud, A., Lemoine, Y., Chiadmi, N., Seasonal variation of growth and agar composition of Gracilaria multipartite harvested along the Atlantic coast of Morocco (1999) Hydrobiologia, 398-399, pp. 167-172
  • Kemsley, E.K., Discriminant analysis of high-dimensional data: a comparison of principal components analysis and partial least squares data reduction methods (1996) Chemometrics Intelligent Lab. Syst., 33, pp. 47-61
  • Kloareg, B., Quatrano, R.S., Structure of the cell wall of marine algae and ecophysiological functions of the matrix polysaccharides (1988) Oceanogr. Mar. Biot. Annu. Rev., 26, pp. 259-315
  • Küpper, F.C., Müller, D.G., Peters, A.F., Kloareg, B., Potin, P., Oligoalginate recognition and oxidative burst play a key role in natural and induced resistance of sporophytes of Laminariales (2002) J. Chem. Ecol., 28, pp. 2057-2080
  • Lahaye, M., Yaphe, W., Effects of seasons on the chemical structure and gel strength of Gracilaria pseudoverrucosa agar (Gracilariaceae, Rhodophyta) (1988) Carbohydr. Polym., 8, pp. 285-301
  • Lawson, C.J., Rees, D.A., An enzyme for the metabolic control of polysaccharides conformation and function (1970) Nature, 227, pp. 390-393
  • Moraudi-Givernaud, A., Givernaud, T., Morvan, H., Cosson, J., Annual variations of the biochemical composition of Gelidium latifolium (Greville) Thuret et Bornet (1993) Hydrobiologia, 260-261, pp. 607-612
  • Morrison, I.M., Hydrolysis of plant cell walls with trifluoroacetic acid (1988) Phytochemistry, 27, pp. 1097-1101
  • Navarro, D.A., Flores, M.L., Stortz, C.A., Microwave-assisted desulfation of sulfated polysaccharides (2007) Carbohydr. Polym., 69, pp. 742-747
  • Nürnberger, T., Brunner, F., Kemmerling, B., Piater, L., Innate immunity in plants and animals: striking similarities and obvious differences (2004) Immunol. Rev., 198, pp. 249-266
  • Ohta, Y., Lee, J.-B., Hayashi, K., Hayashi, T., Isolation of sulfated galactan from Codium fragile and its antiviral effect (2009) Biol. Pharmacol. Bull., 32, pp. 892-898
  • Oliveira, E.C., Saito, R.M., Santos, Neto, J.F., Garófalo, G.M.C., Temporal and spatial variation in agar from a population of Pterocladia capillacea (Gelidiales, Rhodophyta) from Brazil (1996) Hydrobiologia, 326-327, pp. 501-504
  • Orduña-Rojas, J., García-Camacho, K.Y., Orozco-Meyer, P., Ríosmena-Rodríguez, R., Pacheco-Ruiz, I., Zertuche-González, J.A., Meling-López, A.E., Agar properties of two species of Gracilariaceae from the Gulf of California, Mexico (2008) J. Appl. Phycol., 20, pp. 169-175
  • Prado Fernandez, J., Rodríguez Vazquez, J.A., Tojo, E., Andrade, J.M., Quantitation of κ-, ι, and λ-carrageenan by mid-infrared spectroscopy and PLS regression (2003) Anal. Chim. Acta, 480, pp. 23-37
  • Robic, A., Sassi, J.F., Dion, P., Lerat, Y., Lahaye, M., Seasonal variability of physicochemical and rheological properties of ulvan in two Ulva species (Chlorophyta) from the Brittany coast (2009) J. Phycol., 45, pp. 962-973
  • Skriptsova, A.V., Shevchenko, N.M., Zvyagintseva, T.N., Imbs, T.I., Monthly changes in the content and monosaccharide composition of fucoidan from Undaria pinnatifida (Laminariales, Phaeophyta) (2010) J. Appl. Phycol., 22, pp. 79-86
  • Stevenson, T.T., Furneaux, R.H., Chemical methods for the analysis of sulphated galactans from red algae (1991) Carbohydr. Res., 210, pp. 277-298
  • Thero, T., Hartiala, K., Method for determination of the sulfate content of glycosaminoglycans (1971) Anal. Biochem., 41, pp. 471-476
  • Tveter-Gallagher, E., Cogburn, J.N., Mathieson, A.C., Schiff, J.A., Localisation of carrageenan and incorporation of 35SO4 -2 into sulphur amino acids in the red alga Chondrus crispus (1984) Hydrobiologia, 116-117, pp. 488-492
  • Usov, A.I., Structural analysis of red seaweed galactans of agar and carrageenan groups (1998) Food Hydrocoll., 12, pp. 301-308
  • Usov, A.I., Klochkova, N.G., Polysaccharides of algae. 45. Polysaccharide composition of red seaweeds from Kamchatka coastal waters (northwestern Pacific) studied by reductive hydrolysis of the biomass (1992) Bot. Mar., 35, pp. 371-378
  • Verbruggen, H., Leliaert, F., Maggs, C.A., Shimada, S., Schils, T., Provan, J., Booth, D., Species boundaries and phylogenetic relationship within the green algal genus Codium (Bryopsidales) based on plastid DNA sequences (2007) Mol. Phylogenet. Evol., 44, pp. 240-254
  • Weinberger, F., Guillemin, M.L., Destombe, C., Valero, M., Faugeron, S., Correa, J.A., Pohnert, G., Potin, P., Defense evolution in the Gracilariaceae (rhodophyta): substrate-regulated oxidation of agar oligosaccharides is more ancient than the oligoagar-activated oxidative burst (2010) J. Phycol., 46, pp. 958-968
  • Weinberger, F., Leonardi, P., Miravalles, A., Correa, J.A., Lion, U., Kloareg, B., Potin, P., Dissection of two distinct defense-related responses to agar oligosaccharides in Gracilaria chilensis (Rhodophyta) and Gracilaria conferta (Rhodophyta) (2005) J. Phycol., 41, pp. 863-873
  • Weinberger, F., Richard, C., Kloareg, B., Kashman, Y., Hoppe, H.G., Friedlander, M., Structure-activity relationships of oligoagar elicitors toward Gracilaria conferta (Rhodophyta) (2001) J. Phycol., 37, pp. 418-426
  • Wong, K.F., Craigie, J.S., Sulfohydrolase activity and carrageenan biosynthesis in Chondrus crispus (Rhodophyceae) (1978) Plant Physiol, 61, pp. 663-666
  • Wu, Y., Gretz, M.R., Stable isotope labelling method for studies of saccharide metabolism in Agardhiella subulata (1990) Hydrobiologia, 260-261, pp. 595-600

Citas:

---------- APA ----------
Fernández, P.V., Ciancia, M. & Estevez, J.M. (2011) . Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast. Journal of Phycology, 47(4), 802-810.
http://dx.doi.org/10.1111/j.1529-8817.2011.01006.x
---------- CHICAGO ----------
Fernández, P.V., Ciancia, M., Estevez, J.M. "Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast" . Journal of Phycology 47, no. 4 (2011) : 802-810.
http://dx.doi.org/10.1111/j.1529-8817.2011.01006.x
---------- MLA ----------
Fernández, P.V., Ciancia, M., Estevez, J.M. "Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast" . Journal of Phycology, vol. 47, no. 4, 2011, pp. 802-810.
http://dx.doi.org/10.1111/j.1529-8817.2011.01006.x
---------- VANCOUVER ----------
Fernández, P.V., Ciancia, M., Estevez, J.M. Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast. J. Phycol. 2011;47(4):802-810.
http://dx.doi.org/10.1111/j.1529-8817.2011.01006.x